Making method of low-alcohol cider wine
An apple cider and apple juice technology, which is applied in the preparation of alcoholic beverages, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of insufficient aroma of low-alcohol cider, and achieve rich aroma substances, soft taste, Alcohol and harmonizing effects
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[0034] (2) Preparation of apple juice
[0035] Fully ripe, non-rotten apples are fully washed, cut into pieces, and cored, soaked in color-protecting agents (0.05% Vc and 1.5% citric acid) for 15 minutes, and then squeezed separately. Potassium sulfate (60-80mg / L), potassium metabisulfite in apple juice can inhibit bacteria to prevent apple juice from being polluted by bacteria, and resist oxidation to prevent apple juice from browning. At the same time, potassium metabisulfite is added It also has a certain effect on the clarification of apple juice, and then add a certain amount of pectinase (450mg / L) for enzymolysis. The enzymolysis conditions are: enzymolysis temperature 45°C; enzymolysis time: water bath for 2 hours, and then suction filtration to obtain clarification apple juice.
[0036] (3) Brewing of cider
[0037] Preparation of yeast seed liquid: inoculate WLS21 yeast strains in YPD medium (1% yeast extract powder, 2% glucose, 2% peptone), and activate them by cul...
Embodiment 1
[0098] (1) Take WLS21 Saccharomyces cerevisiae preserved on the slant and carry out streak culture on YPD plate medium, the culture temperature is 24-30°C, and the culture time is 24h;
[0099] (2) Liquid culture: Pick two rings of yeast strains activated for 24 hours and inoculate them into YPD liquid medium. The culture conditions are: 24-30°C, 24 hours, 120rpm;
[0100] (3) Saccharomyces seed culture liquid: draw the seed liquid cultivated in YPD liquid medium and add in the 500ml triangular flask that 400ml freshly squeezed apple juice is housed by 8% inoculum size and carry out expansion cultivation and obtain fermented seed liquid, culture condition is : 24~30 ℃, 24h, 120rpm, the soluble solid content of the freshly squeezed apple juice used in this embodiment is 10~13 ° Brix;
[0101] (4) Fermentation of low-alcohol cider: put 400ml of freshly squeezed apple juice in a 500ml triangular flask, ferment at 22.90°C, adjust the pH of the apple juice to 4.65, and activate the...
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