Extensive and precise quantitative detection methods of milk components in goat milk powder
A quantitative detection method and technology of goat milk powder, which can be applied to the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve problems such as failure to accurately determine the specific content of components, achieve a low detection limit, and improve amplification efficiency. , a strong specific effect
Active Publication Date: 2019-10-25
SHAANXI NORMAL UNIV
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Problems solved by technology
Domestic scholars use PCR amplification technology to detect bovine-derived and sheep-derived components in raw milk, but they can only determine whether they contain components of cattle, sheep and other species, but cannot accurately determine the specific content of the components, and there is no Research in Milk Powder
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[0029] The present invention will be described in more detail below in conjunction with specific experiments and examples, but the present invention is not limited to the following implementation situations.
[0030] (1) Experimental materials
[0031] 1) Sample source
[0032] No added full-fat pure cow and goat milk powder.
[0033] 2) Test reagent
[0034] Phosphate buffer solution (PBS), OP emulsifier, DNA extraction buffer, 20% sodium dodecyl sulfate (SDS) lysate, proteinase K (10mg / mL), Tris saturated phenol, chloroform isoamyl alcohol (24: 1), phenol, chloroform and isoamyl alcohol (25:24:1), ice absolute ethanol (-20°C), 70% ice ethanol (-20°C), TE buffer, TAE buffer, agarose, 2 ×Ultal SYBRM Mixture, 2×Es Taq Master Mixture.
[0035] 3) Main instruments
[0036] Ultra-high-speed low-temperature centrifuge (3k30, Sigma, USA), low-speed large-capacity centrifuge (DL-4C, Shanghai Anting Scientific Instrument Factory), precision micropipette gun (Eppendorf, Germany), ...
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The invention relates to an extensive and accurate quantitative detection method for a milk component in goat milk powder. The extensive and accurate quantitative detection method for the milk component in the goat milk powder comprises the following steps: classifying adulteration models through common PCR; obtaining a relational expression of Ct values in various corresponding grades and an adulteration ratio by using fluorescent quantitation PCR; determining the grade of a sample to be detected through the common PCR; then carrying out fluorescent quantitation PCR on the sample to be detected to acquire a Ct value; substituting the Ct value into a relation equation of a corresponding grade so as to calculate percentage composition of the milk component in the goat milk powder. The common PCR is combined to real-time fluorescent quantitation PCR, qualitative, extensive and accurate quantitative detection of the milk component in the goat milk powder can be finished, the method is carried out step by step, the cost is gradually increased, and the method can meet demands of different levels, is high in sensitivity, high in specificity and low in detection limit, can be popularized to be used for detecting adulteration in all animal powder preparations, and can also be used as a reference for detection of components of animal origin in other animal products.
Description
technical field [0001] The invention belongs to the technical field of food safety detection, and in particular relates to a method for extensive and precise quantitative detection of milk components in goat milk powder. Background technique [0002] In recent years, incidents of adulteration of dairy products have been common. In most cases, cheap and easy-to-obtain raw materials are mixed with higher-priced raw materials for sale in order to obtain higher profits, such as adding cow milk and goat milk to buffalo milk. Add milk, add soy milk to milk, etc. Food adulteration will not only cause economic losses to consumers, but also may involve some special medical requirements, food allergies and religious beliefs. [0003] Many methods (such as electrophoresis, immunological techniques, chromatography and mass spectrometry, etc.) that have emerged to identify adulteration of dairy products can solve the above-mentioned related problems to a certain extent, but in terms of ...
Claims
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IPC IPC(8): C12Q1/6851
CPCC12Q1/6851C12Q2531/113C12Q2563/107
Inventor 刘永峰廖晶
Owner SHAANXI NORMAL UNIV