Method for preparing steroid immunosensor based on sheet black phosphorus and application

An immunosensor and steroid technology, which is used in the preparation and application of steroid immunosensors based on flake black phosphorus, and achieves the effects of lowering the detection limit, increasing the electron transmission capacity, and increasing the loading capacity.

Active Publication Date: 2016-10-12
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods require large-scale equipment and professional instruments, and professionally trained technicians are required to operate

Method used

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Examples

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Embodiment 1

[0024] Preparation method and application of a steroid immunosensor based on flake black phosphorus

[0025] (1) A glassy carbon electrode with a diameter of 3 mm is covered with Al 2 o 3 Grinding with polishing powder, washing with ultrapure water; adding 1 µL, 1 mg / mL black phosphorus@ionic liquid P@IL composite material dropwise to the electrode surface, washing with ultrapure water, and drying at room temperature to form a film;

[0026] (2) Set the projected area to 5 mm 2 The porous PtAu alloy film is covered on the electrode surface and rinsed with ultrapure water;

[0027] (3) Drop 1 µL of steroid antibody standard solution on the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C;

[0028] (4) Add 1 µL of bovine serum albumin BSA solution with a mass fraction of 0.5% dropwise on the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C;

[0029] (5) Add 1 µL, 0.005-20 ng / mL of a series of steroid...

Embodiment 2

[0031] Preparation method and application of a steroid immunosensor based on flake black phosphorus

[0032] (1) A glassy carbon electrode with a diameter of 3 mm is covered with Al 2 o 3 Grinding with polishing powder, washing with ultrapure water; adding 4 μL, 4 mg / mL black phosphorus@ionic liquid P@IL composite material dropwise to the electrode surface, washing with ultrapure water, and drying at room temperature to form a film;

[0033] (2) Set the projected area to 7 mm 2 The porous PtAu alloy film is covered on the electrode surface and rinsed with ultrapure water;

[0034] (3) Drop 5 µL of steroid antibody standard solution on the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C;

[0035] (4) Add 5 µL of bovine serum albumin BSA solution with a mass fraction of 1.0% dropwise on the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C;

[0036] (5) Add 5 µL, 0.005-20 ng / mL of a series of steroid...

Embodiment 3

[0038] Preparation method and application of a steroid immunosensor based on flake black phosphorus

[0039] (1) A glassy carbon electrode with a diameter of 3 mm is covered with Al 2 o 3 Grinding with polishing powder, washing with ultrapure water; adding 10 µL, 10 mg / mL black phosphorus@ionic liquid P@IL composite material dropwise to the electrode surface, washing with ultrapure water, and drying at room temperature to form a film;

[0040] (2) Set the projected area to 10 mm 2 The porous PtAu alloy film is covered on the electrode surface and rinsed with ultrapure water;

[0041] (3) Add 10 µL of steroid antibody standard solution dropwise to the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C;

[0042] (4) Add 10 µL of bovine serum albumin BSA solution with a mass fraction of 1.5% dropwise on the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C;

[0043] (5) Add 10 µL, 0.005-20 ng / mL of a ser...

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Abstract

The invention relates to a method for preparing a steroid immunosensor based on sheet black phosphorus and application and belongs to the technical field of novel biosensing and detection. The sheet black phosphorus is provided with a structure having the property similar to graphene and has good conductivity. Ionic liquid serves as a dispersing agent of the sheet black phosphorus and is applied to a base of the immunosensor. A porous PtAu alloy film is utilized to firm combine a base layer material and an antibody, and the stability of the sensor is enhanced. Base solution potassium ferricyanide is utilized as signals, and ultrasensitive detection of steroid is performed.

Description

technical field [0001] The invention relates to a preparation method and application of a steroid immune sensor based on flake black phosphorus. Black phosphorus@ionic liquid composite material is used as a detection base material to enhance conductivity and increase the solidity of signal molecules on the sensing surface. In terms of loading capacity, an electrochemical immunosensor for detecting steroids is prepared by modifying a porous PtAu alloy film, which belongs to the technical field of new functional materials and biosensing detection. technical background [0002] Steroids are a general term for a large class of cyclopentyl-condensed perhydrophenanthrene derivatives widely distributed in the biological world. As far as estradiol is concerned, it can supplement estrogen deficiency and is often used to treat female hypogonadism, after bilateral oophorectomy, and menopausal syndromes such as hot flashes, sweating, and mental and neurological symptoms. But there will...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/92G01N27/327
CPCG01N27/3275G01N33/92
Inventor 魏琴任祥张勇李燕杜斌胡丽华庞雪辉范大伟
Owner UNIV OF JINAN
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