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Method for preparing oligomeric proanthocyanidins by utilizing grape seed extract

A technology of oligomeric proanthocyanidins and proanthocyanidins, which is applied in the field of separation and preparation of proanthocyanidins, can solve the problems of difficulty in extracting oligomeric proanthocyanidins and high polymeric proanthocyanidins, limited separation precision of macroporous adsorption resin, and low yield of oligomeric proanthocyanidins. Purpose-defined, color-stable, high-performance results

Active Publication Date: 2016-10-26
HUNAN XINLI BIOLOGICAL SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantage of this method is: proanthocyanidins are soluble in water and ethanol, and are not affected by pH, so it is difficult to extract monomers, oligomers and high polymers of proanthocyanidins in the pH range of 4.0 to 4.4; in addition, macroporous The separation accuracy of the adsorption resin is limited, and the monomer, oligomer and high polymer proanthocyanidin cannot be completely separated, and it is difficult to obtain the oligomer product with a polymerization degree of only 2-4
The main disadvantage of this method is that the high-polymer proanthocyanidins can only be partially separated by cold dissolution; although the high-polymer proanthocyanidins can be completely removed by nanofiltration, the molecular weight cut-off interval distribution of the nanofiltration membrane will reduce the degree of polymerization to 3-5. Oligomer proanthocyanidins interception, low yield of oligomer proanthocyanidins, large waste of raw materials, high production cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Preparation of ethanol solution of proanthocyanidins

[0026]Weigh a certain amount of grape seed extract, according to the ratio of grape seed extract mass / dehydrated ethanol volume ratio of 1:50 (kg / L), add it to dehydrated ethanol under stirring, and suction filter after 10 minutes of treatment , respectively collect the filter residue and filtrate, the collected filter residue mainly contains high-polymer proanthocyanidins with a degree of polymerization exceeding 10, which is used to prepare feed antioxidants; the collected filtrate is the ethanol solution of proanthocyanidins, which is used for the next step to prepare primary separation and concentration liquid.

[0027] (2) Preparation of primary separation concentrate

[0028] After step (1) is completed, the ethanol solution of proanthocyanidins collected in step (1) is pumped into a vacuum concentrator, and concentrated in a vacuum at a temperature of 60° C. and a vacuum of 0.06 MPa until the volume of t...

Embodiment 2

[0038] (1) Preparation of ethanol solution of proanthocyanidins

[0039] Weigh a certain amount of grape seed extract, according to the ratio of grape seed extract mass / dehydrated ethanol volume ratio of 1:75 (kg / L), add it to dehydrated ethanol under stirring, and suction filter after 20 minutes of treatment , respectively collect the filter residue and filtrate, the collected filter residue mainly contains high-polymer proanthocyanidins with a degree of polymerization exceeding 10, which is used to prepare feed antioxidants; the collected filtrate is the ethanol solution of proanthocyanidins, which is used for the next step to prepare primary separation and concentration liquid.

[0040] (2) Preparation of primary separation concentrate

[0041] After step (1) is completed, the ethanol solution of proanthocyanidins collected in step (1) is pumped into a vacuum concentrator, and concentrated in a vacuum at a temperature of 70° C. and a vacuum of 0.07 MPa until the volume of ...

Embodiment 3

[0051] (1) Preparation of ethanol solution of proanthocyanidins

[0052] Weigh a certain amount of grape seed extract, according to the ratio of grape seed extract mass / dehydrated ethanol volume ratio of 1:100 (kg / L), add it to dehydrated ethanol under stirring, and suction filter after 30min , respectively collect the filter residue and filtrate, the collected filter residue mainly contains high-polymer proanthocyanidins with a degree of polymerization exceeding 10, which is used to prepare feed antioxidants; the collected filtrate is the ethanol solution of proanthocyanidins, which is used for the next step to prepare primary separation and concentration liquid.

[0053] (2) Preparation of primary separation concentrate

[0054] After step (1) is completed, pump the ethanol solution of proanthocyanidins collected in step (1) into a vacuum concentrator, and carry out vacuum concentration at a temperature of 80° C. and a vacuum of 0.08 MPa until the volume of the concentrated s...

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Abstract

The invention provides a method for preparing oligomeric proanthocyanidins by utilizing a grape seed extract, belonging to the technical field of separation and preparation of the oligomeric proanthocyanidins. According to the invention, oligomeric proanthocyanidins freeze-dried powder and polymeric proanthocyanidins freeze-dried powder are prepared through dissolving, filtering, vacuum concentration, ultrafiltration, column filling, elution, nanofiltration and freeze-drying by using the grape seed extract as a raw material. The method provided by the invention has high raw material utilization rate, high separation accuracy, good separation effect, high product purity and clear product use, realizes complete utilization of raw materials and complete recycling of the solvent, is free of three-wastes discharge, and is a typical high-tech green process. The oligomeric proanthocyanidins prepared by using the method provided by the invention can be extensively applied in the fields of medicines, health-care products, etc.

Description

1. Technical field [0001] The invention belongs to the technical field of separation and preparation of proanthocyanidins, and in particular relates to a method for preparing oligomeric proanthocyanidins from grape seed extract. 2. Background technology [0002] Oligomeric proanthocyanidins are derived from plants and are composed of proanthocyanidins with a degree of polymerization lower than 5. They have the advantages of anti-oxidation, anti-radiation, and chelated metal ions. They are widely used in medicine and health care. biologically active substances. [0003] There are existing methods for preparing oligomeric proanthocyanidins, such as the invention patent with the application number CN201410140039.3 and the name "a method for preparing oligomeric proanthocyanidins". The method uses 60-70% ethanol at pH 4.0-4.4 The monomer, oligomer and high polymer proanthocyanidin are extracted, and then adsorbed and separated by macroporous resin, and the oligomer product with...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62C12G1/00
CPCC07D311/62C12G1/00
Inventor 徐扬陶仁友江文杨洋石业新袁德宽余红梅周小华
Owner HUNAN XINLI BIOLOGICAL SCI & TECH
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