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Method of purifying to prepare high-purity xylobiose

A xylobiose, high-purity technology, applied in the field of purification and preparation of high-purity xylobiose, can solve the problems of high price, and achieve the effect of simple method, low cost, and favorable promotion, development and application

Active Publication Date: 2016-10-26
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Only its standard products are sold in the domestic and foreign markets, and the price is expensive

Method used

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  • Method of purifying to prepare high-purity xylobiose
  • Method of purifying to prepare high-purity xylobiose
  • Method of purifying to prepare high-purity xylobiose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1, the optimization of the conditions of purifying and preparing high-purity xylobiose

[0034] 1. Selection of filling materials for decolorization and desalting columns

[0035] Purify and prepare high-purity xylobiose according to the following steps:

[0036] (1) Ultrafiltration: filter the xylan enzymolysis solution prepared by alkali pretreatment bagasse with an ultrafiltration membrane (molecular weight cut-off is 10000Da, pore size is 0.001μm) (operating pressure is 0.15MPa), collect the filtrate, remove large Molecular substances and some non-sugar substances. Wherein, the steps of alkali pretreatment of the xylan enzymatic hydrolyzate prepared from bagasse refer to the literature "Xue, JL, Zhao, S, et al.A biotechnological process efficiently co-produces two highvalue-added products, glucose and xylooligosaccharides, from sugarcanebagasse .The method disclosed in "Bioresourse Technology, 2016, 204, 130-138", specifically as follows: according to t...

Embodiment 2

[0076] Embodiment 2, purification prepares high-purity xylobiose

[0077] Purification and preparation of high-purity xylobiose under the optimized conditions in Example 1, the specific operations are as follows:

[0078] (1) ultrafiltration: the same as the operation of step (1) in the above-mentioned one.

[0079] (2) Flow the filtrate through the ion exchange resin column (ion exchange resin column conditions: the series sequence of the filler is 001-7--D201, the filling volume V(001-7):V(D201)=1.67:1, sample injection The flow rate is 1.76mL / min), and the DNS method is used to determine the time to start and stop collecting the filtrate, and the collected liquid is the purified liquid of the xylan enzymatic hydrolysis liquid.

[0080] (3) The liquid obtained in step (2) of taking 5% column bed volume is pumped into the well-balanced gel chromatography (Sephadex G-10 column with a height-to-diameter ratio of 34.03:1), and all the liquid enters After the column bed, use 1....

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Abstract

The invention discloses a method of purifying to prepare high-purity xylobiose. The method includes following steps: (1), performing ultrafiltration on xylan enzymatic hydrolysate, and collecting filtrate; (2), enabling the filtrate to flow through an ion exchange resin column to obtain purified liquid of the xylan enzymatic hydrolysate; (3), subjecting the purified liquid to gel filtration chromatography to obtain xylobiose. By utilizing the xylan enzymatic hydrolysate prepared from bagasse subjected to separation, purification and alkali treatment, high-purity xylobiose can be prepared while xylotriose and xylose are co-generated, purities of xylobiose, xylotriose and xylose are 95.74+ / -1.43%, 85.11+ / -2.27% and 86.61+ / -3.57%, and loss of xylobiose is lowest, being 6.38%. The method is simple, convenient, flexible, practical, quick, low in cost and conducive to popularization in development and application.

Description

technical field [0001] The invention relates to the field of separation and purification of oligosaccharides, in particular to a method for purifying and preparing high-purity xylobiose. Background technique [0002] Xylo-oligosaccharides are oligosaccharides formed by connecting 2 to 7 xyloses with β-1,4 glycosidic bonds. They have the characteristics of low heat, stability, non-toxicity and unique physiological functions. Xylo-oligosaccharides can promote the proliferation of bifidobacteria in the intestinal tract of humans and animals, inhibit the growth of harmful bacteria, and promote the absorption of calcium. DF, et al. Xylo-oligosaccharides from lignocellular materials: Chemical structure, health benefits and production by chemical and enzymatic hydrolysis [J]. Food Research International, 2013, 51: 75-85). The sweetness of xylobiose is about 40% of that of sucrose, and the sweetness of xylooligosaccharide products with a content of 50% is about 30% of that of sucro...

Claims

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Application Information

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IPC IPC(8): C07H3/04C07H3/06C07H3/02C07H1/06
CPCC07H1/06C07H3/02C07H3/04C07H3/06
Inventor 冯家勋赵帅蒋随新
Owner GUANGXI UNIV