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Benzoyl urea insecticide degrading bacteria, bacterial agent by using same and application of benzoyl urea insecticide degrading bacteria

A technology of benzoyl urea and insecticide, which is applied in the field of benzoyl urea insecticide degrading bacteria and the bacterial agent produced by it, can solve the problems of no degradation and other problems, and achieve the protection of health and good removal effect , Reduce the effect of phytotoxicity on crops

Active Publication Date: 2016-11-09
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there are no research and application reports on bioremediation technology patents that can degrade various benzoylurea insecticides such as hexaflumuron, diflubenzuron, and diflubenzuron

Method used

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  • Benzoyl urea insecticide degrading bacteria, bacterial agent by using same and application of benzoyl urea insecticide degrading bacteria
  • Benzoyl urea insecticide degrading bacteria, bacterial agent by using same and application of benzoyl urea insecticide degrading bacteria
  • Benzoyl urea insecticide degrading bacteria, bacterial agent by using same and application of benzoyl urea insecticide degrading bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Isolation and Characterization of Strain M6:

[0032] The enrichment matrix used to enrich the benzoylurea insecticide-degrading strain was taken from the activated sludge of the pesticide wastewater biochemical treatment tank of Hainan Runhe Pesticide Co., Ltd., Haikou, Hainan, and 5.0 g of the activated sludge was added to the basic salt medium In, add 50mg·L -1 Diflubenzuron, cultured at 30°C and 180rpm for 7 days, transferred to the same culture medium with a 5% inoculum size, transferred continuously for 3 times, and diluted the enrichment solution in gradient, taking 10 -4 ~10 -7 0.1mL of each diluted enrichment solution was applied to the -1 On the solid medium plate of diflubenzuron, after culturing at 30°C for 4 days, pick the grown single colony and inoculate it in a medium containing 100mg·L -1 Various benzoylurea insecticides (hexaflumuron, diflubenzuron, and diflubenzuron) were cultured at 30°C and 180 rpm for 5 days to verify their degradation effects. ...

Embodiment 2

[0037] Biodegradation test of bacterial strain M6 to benzoylurea insecticides in medium:

[0038] In the basal salt culture medium (with embodiment 1) respectively, add final concentration and be 100mg L -1 Hexaflumuron, diflubenzuron, and diflubenzuron were inserted into the bacterial strain M6 bacterium liquid according to the inoculation amount of 1% volume ratio, and the final concentration of 100mg L was added to the basic salt medium (same as Example 1) respectively. -1 Hexaflumuron, diflubenzuron, and diflubenzuron were used to inoculate the inactivated bacterial strain M6 with an inoculation amount of 1% volume ratio as control 1, control 2, and control 3, cultivated in a constant temperature shaker at 30°C, and regularly sampled. The method for verification of the degradation effect in Example 1 detects the degradation of hexaflumuron, diflubenzuron, and diflubenzuron by high-performance liquid chromatography, and the HPLC analysis spectrum of the degradation result i...

Embodiment 3

[0043] Preparation method of degrading bacteria agent

[0044] 1) Inoculate the cultured test tube solution of the strain M6 into the fermentation medium with an inoculation amount of 1% by volume, and vibrate and cultivate to the logarithmic phase to obtain the fermentation strain;

[0045] 2) Inoculate 500 liters of 70% (based on the volume of the fermenter, the same below) with an inoculation amount of 8% (v / v, based on the volume of the culture medium) of the above-mentioned cultured fermentation strains into 500 liters of liquid Cultivate in the medium of the seed tank, cultivate to the logarithmic growth phase, and obtain the seed liquid;

[0046] 3) The inoculation amount of the seed solution is 8% (v / v, based on the volume of the medium, the same below) into the culture medium of the production tank with a liquid volume of 70%.

[0047] 4) During the cultivation process of seed tanks and production tanks, the ventilation rate of sterile air is 1:0.8, the stirring spee...

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Abstract

The invention discloses a benzoyl urea insecticide degrading bacterial strain M6. The benzoyl urea insecticide degrading bacterial strain M6 is identified as achromobacter dolens, is preserved in the China Center for Type Culture Collection on May 11, 2016, and has a preservation number of CCTCC NO: M 2016257. The invention further discloses an application of the bacterial strain M6 in production of a benzoyl urea insecticide degrading bacterial agent, the degrading bacterial agent can degrade a variety of benzoyl urea insecticides such as hexaflumumron, diflubenzuron and chlorbenzuron, and the degradation rate of residual hexaflumumron, diflubenzuron and chlorbenzuron in soil or a water body environment can be up to 90% or above in a short time; the degrading bacterial agent can be produced by using general fermentation equipment of the fermentation industry, and has the advantages of low production cost, convenient use and good removal effects.

Description

technical field [0001] The invention relates to the restoration of environmental pollution microorganisms, in particular to a benzoylurea insecticide-degrading bacterium and its production and application. Background technique [0002] Benzoylurea insecticides, including hexaflumuron, diflubenzuron, and diflubenzuron, etc., are chitin insecticidal inhibitors, which cause insect death by inhibiting chitin synthesis. Benzoylurea insecticides show good insecticidal activity against metamorphic insects, especially Lepidoptera larvae, and are used to control a variety of pests, such as armyworm, beet armyworm, litura, poisonous moth, cabbage caterpillar , pine caterpillar, diamondback moth, rice leaf roller, pear borer, etc., and has good effects on pests resistant to organophosphorus, carbamates, and pyrethroids, and can also be used together with abamectin, etc. It is widely used in rice, Chinese cabbage, rape, lychee, longan, tea and other crops to prevent and control the pal...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02A62D101/04A62D101/26C12R1/025
CPCA62D3/02A62D2101/04A62D2101/26C12N1/20C12N1/205C12R2001/025
Inventor 李怡单颖李勤奋武春媛邓晓
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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