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Colorimetric sensor for detecting mercury ions and preparation method thereof

A colorimetric sensor and mercury ion technology, applied in the field of sensors, can solve the problems of complicated instrument operation, and achieve the effects of good repeatability, mild reaction conditions and simple preparation method

Inactive Publication Date: 2016-11-09
UNIV OF JINAN
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0004] The invention provides a colorimetric sensor for mercury detection aiming at the shortcomings of complex instrument operation and the need for professional operators in the existing detection method

Method used

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  • Colorimetric sensor for detecting mercury ions and preparation method thereof
  • Colorimetric sensor for detecting mercury ions and preparation method thereof
  • Colorimetric sensor for detecting mercury ions and preparation method thereof

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Embodiment 1

[0038] The preparation method of described biosensor comprises the following steps:

[0039] (1) Hybridization of DNA probes;

[0040] (2) Amplify the signal of the detected specific sequence;

[0041] (3) Perform colorimetric detection on the sequence.

[0042] In the preparation method, the specific operation steps of step (1) are as follows: incubate the mixture of 2 μL Probe 1 and 2 μL Probe 2 at 37° C. for 0.5 h.

[0043] In the preparation method, the preferred steps of modifying the homogeneous reaction product onto the electrode surface are as follows:

[0044] Take out the mixed solution incubated in (1), add dNTPs, phi29DNA polymerase and Nb.BbvCI endonuclease to the mixed solution, shake for 30s, and incubate in a 37°C incubator for 1h;

[0045] In the preparation method, the addition of the enzyme is carried out at low temperature to ensure the activity of the enzyme.

[0046] Before detection, a large amount of heme aptamer produced in step (2) was mixed with ...

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Abstract

The invention relates to a colorimetric sensor for detecting mercury ions. A preparation method for the colorimetric sensor comprises the following steps: hybridization of probes; signal amplification; and colorimetric detection. The colorimetric sensor prepared in the invention realizes highly-specific detection of target mercury ions; and in virtue of nucleic acid tool enzyme, signal amplification effect is exerted.

Description

technical field [0001] The invention relates to the technical field of sensors, in particular to a colorimetric sensor for detecting mercury ions. Background technique [0002] Mercury is a silvery white shiny heavy liquid, chemically stable, insoluble in acid and alkali. Mercury can evaporate at room temperature, and mercury vapor and mercury compounds are highly toxic (chronic). Mercury is a naturally occurring element found in air, water and soil. Mercury is a highly toxic non-essential element widely present in various environmental media and food chains (especially fish), and its traces can be found all over the world. Mercury can accumulate in organisms and is easily absorbed through the skin as well as the respiratory and digestive tracts. Minamata disease is a type of mercury poisoning. Mercury damages the central nervous system and has adverse effects on the mouth, mucous membranes and teeth. Prolonged exposure to high levels of mercury can cause brain dama...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/33
CPCG01N21/33G01N21/78
Inventor 王玉崔雪君黄加栋刘素冉令芝郭玉娜邱婷婷
Owner UNIV OF JINAN
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