Detection method and application of biomarker for detecting cerebral apoplexy incidence risk and incidence

A technology of biomarkers and detection methods, which is applied in the field of detecting the risk of stroke and the biomarkers of the onset, to achieve the effect of high cost performance, benefit popularization, and reduce the time of detection

Inactive Publication Date: 2016-11-09
昂朗生物科技(上海)有限公司
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Problems solved by technology

The relationship between the concentration of t

Method used

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  • Detection method and application of biomarker for detecting cerebral apoplexy incidence risk and incidence
  • Detection method and application of biomarker for detecting cerebral apoplexy incidence risk and incidence
  • Detection method and application of biomarker for detecting cerebral apoplexy incidence risk and incidence

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Experimental program
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Embodiment Construction

[0020] 1. Experimental method:

[0021] 1. Sample collection Take 3ml of urine from stroke patients, high-risk groups of stroke and normal people.

[0022] 2. Add it to the reaction bottle containing 0.5ml of chromogenic solution. The specific reaction in the bottle is as follows:

[0023] a. Mercury sulfate is completely ionized and used in the reaction as a mercury source, tyrosine is mercuryized, and the reaction formula is:

[0024]

[0025] b. Mercury ions are replaced by nitroso into 3-nitroso-4-hydroxyphenylalanine, freeing mercury ions, the reaction formula:

[0026]

[0027] c. Mercury ions chelate with 3-nitroso-4-hydroxyphenylalanine to form a brick-red chelate (color-developing substance), and determine the tyrosine content according to the color of the chelate. Reaction formula:

[0028]

[0029] 3. Let stand for 5 minutes after shaking slightly.

[0030] 4. Compare the color of the precipitate with the standard color plate to judge the result.

[00...

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Abstract

The invention provides a detection method and application of a biomarker for detecting the cerebral apoplexy incidence risk and the cerebral apoplexy incidence based on the tyrosine level of a body fluid. The method comprises the following steps: 1, detecting the tyrosine concentration of exosome in a sample; 2, comparing the tyrosine concentration in step 1 with the tyrosine concentration result of known cerebral apoplexy patients, cerebral apoplexy high-risk populations and healthy populations; and 3, completing the detection method based on the tyrosine level of the body fluid as the biomarker for detecting the cerebral apoplexy incidence risk and the cerebral apoplexy incidence according to the above obtained comparison result. The content of tyrosine in exosome, such as urine, sweat and saliva, of the body fluid of cerebral apoplexy incidence or high-risk population is detected in order to predicate and detect the cerebral apoplexy incidence. The tyrosine level is positively correlated to the cerebral apoplexy incidence and the cerebral apoplexy risk.

Description

technical field [0001] The present invention relates to the detection of stroke risk and biomarkers, specifically based on the detection of tyrosine levels in body fluids, as a detection method and application for detecting stroke risk and biomarkers. Background technique [0002] In my country and other countries in the world, stroke is one of the most serious diseases that cause death and lifelong disability. Ischemic stroke is a type of disease with the highest incidence rate of stroke. At present, the main means of diagnosis is to carry out imaging analysis through nuclear magnetic resonance technology. This method can only be tested in a hospital, which is very inconvenient for patients, takes a relatively long time and costs a lot. Therefore, inventing a new, simple, fast and home-use stroke detection method has great socio-economic significance and clinical value. [0003] Tyrosine is an aromatic amino acid and one of the 20 proteinogenic amino acids used to synthe...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N30/02G01N21/64G01N33/68
CPCG01N21/78G01N21/64G01N30/02G01N33/6806G01N2800/50
Inventor 殷卫海吴丹红蔡林卫张铭超
Owner 昂朗生物科技(上海)有限公司
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