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Novel method for identifying toxoplasma, rubella virus and herpes simplex virus at same time

A herpes simplex virus and rubella virus technology, applied in the field of detection and identification, can solve the problems of wrong judgment of pathogens, unclear identification of pathogens, etc., and achieve the effects of accurate results, saving inspection costs, and uniform standards

Active Publication Date: 2016-11-09
BINZHOU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When using classical methods, there is a possibility of errors that sometimes lead to unclear identification of the pathogen and thus to a wrong diagnosis of the pathogen

Method used

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  • Novel method for identifying toxoplasma, rubella virus and herpes simplex virus at same time
  • Novel method for identifying toxoplasma, rubella virus and herpes simplex virus at same time
  • Novel method for identifying toxoplasma, rubella virus and herpes simplex virus at same time

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] A method for simultaneously detecting and / or identifying toxoplasma gondii, rubella virus and herpes simplex virus, comprising the following steps:

[0068] 1. Optimization of silicon wafer surface modification methods:

[0069] This embodiment has adopted a variety of modification methods, and now two kinds of modification methods are screened for optimization. The steps are as follows:

[0070] 1.1 Rinse the wafer with deionized water 3 times, soak the wafer with 1mL of aminosilane and 3mL of concentrated sulfuric acid for half an hour, rinse the wafer with PBS 3 times, soak the wafer with 1mL of glutaraldehyde and 3mL of PBS for 1 hour, and obtain the chemical properties of aldehyde groups on the surface chip.

[0071] 1.2 Rinse the silicon wafer 3 times with deionized water, soak the silicon wafer with 1mL of aminosilane and 3mL of concentrated sulfuric acid for half an hour, rinse the silicon wafer with anhydrous alcohol for 3 times, add saturated sodium succinate...

Embodiment 2

[0122] A protein chip for ellipsometric microscopic imaging, which is prepared by the following method:

[0123] Assembling identification probes on silicon wafers with carboxyl-modified surfaces: assembling the antigens of toxoplasma gondii, rubella virus and herpes simplex virus on the detection sites, and then washing with PBS; then blocking the detection sites with blocking reagents to obtain Ellipsometric microscopic imaging protein chip; preparation parameters refer to the parameters in the standardized detection and / or identification procedures in Example 1.

[0124] Quantitative detection of rubella virus antibody

[0125] 1. Calibration curve and quantitative detection of clinical serum samples

[0126] To establish a calibration curve, serum samples (No. 942, 218 IU / mL) were serially diluted, and the diluted concentration was 0011, 0043, 0170, 0681, and 2725IU / mL5 concentration gradients of rubella virus antibody, diluted with PBST. Rubella virus antigen was immobi...

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Abstract

A disclosed novel method for identifying toxoplasma, rubella virus and herpes simplex virus at same time comprises (1) preparing an identification chip; (2) injecting a to-be-identified solution into detection sites of the identification chip in the step (1), after an antibody molecule in the to-be-identified solution is specifically combined with an antigen molecule on the identification chip and accordingly the thickness or density of the molecular film layer on the surface of the detection sites of the identification chip is changed, performing flushing; and (3) detecting the variation in the step (2) by using an ellipsometric microscopy imaging instrument, so as to determine whether the to-be-identified solution contain toxoplasma, rubella virus and herpes simplex virus and obtain the antibody content of toxoplasma, rubella virus and herpes simplex virus. The method is capable of rapidly accurately qualitatively and / or quantitatively determining toxoplasma, rubella virus and herpes simplex virus.

Description

technical field [0001] The invention belongs to the technical field of detection and identification, and in particular relates to a new method for simultaneously identifying toxoplasma gondii, rubella virus and herpes simplex virus. Background technique [0002] Toxoplasma gondii is an obligate intracellular parasite, subclass Coccidia, order Eucoccidia, Isospora Coccidiaceae, genus Toxoplasma. The worm is distributed worldwide and can be infected by humans and many animals. It can parasitize in all nucleated cells except red blood cells, flow with the blood, reach all parts of the body, damage the brain, heart, and fundus of the eyes, resulting in a decline in the body's immunity. suffering from various diseases. After the human body is infected, it is mostly in a state of recessive infection. Only when the body's immunity is low, the worm body is activated and rapidly proliferates, and the pathogenicity is enhanced. Since TOX poses a great threat to human health, the tim...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/569G01B11/06
CPCG01B11/0625G01N33/56905G01N33/56983G01N33/6803G01N2333/19G01N2333/45
Inventor 孙红柳王巧云
Owner BINZHOU MEDICAL COLLEGE
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