Artificial regeneration method of madrepore
A kind of coral and artificial technology, applied in fish farming, application, climate change adaptation, etc., can solve problems such as ecosystem impact, and achieve the effects of enhancing resistance, fast growth, and low cost
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Embodiment 1
[0027] The regeneration method of stony coral comprises the following steps:
[0028] 1. Collection of small pieces of coral: In the natural distribution area of corals along the eastern coast of Shenzhen, from June to September 2015, we collected small pieces of small branches of healthy staghorn corals with a length of about 2 to 3 cm around the mother plant with restricted growth, and kept them temporarily in the aquarium in the box.
[0029]2. Sterilization and disinfection of small coral pieces: Put the small pieces of coral in the culture tank and wash them with sterilized seawater for 2 minutes, then soak them in the special disinfectant containing Seachem (sea chemical) Reef Dip from low to high concentration. The concentration of the disinfectant is in order 5ppm, 10ppm, 20ppm, 40ppm and 80ppm, soak for 30 seconds in each concentration, and then put the coral pieces in a circulating water tank and rinse with sterilized seawater for 2 minutes.
[0030] 3. Preparatio...
Embodiment 2
[0039] The experimental method is the same as in Example 1, the only difference is that step (4) is carried out as follows:
[0040] (1) Cut a 5.5 cm thick granite slab into units of 55 cm x 85 cm x 5.5 cm. Drill holes in the granite slab at intervals of 15 cm and a depth of 5 cm for planting small pieces of coral.
[0041] (2) Put the 55 cm × 85 cm × 5.5 cm granite culture plate in the circulating water tank, water will flow in from the water outlet to replenish, add water to cover the culture plate for about 50 cm, and install the culture plate on the wall plate at a distance of 20 cm from the water surface. Wave machine, making waves at a frequency of 15 times per minute. Add calcium carbonate and calcium chloride to make the calcium concentration 600ppm, and make the alkalinity 6meq / L, pH 8.0; connect CO 2 Storage tank and gas pump, regulating CO 2 The concentration is 33ppt, the dissolved oxygen is greater than 8mg / L; the ammonia value is guaranteed to be less than 0.1p...
Embodiment 3
[0044] The experimental method is the same as in Example 1, the only difference is that step (4) is carried out as follows:
[0045] (1) Cut a 5.5 cm thick granite slab into units of 55 cm x 85 cm x 5.5 cm. Drill holes in the granite slab at intervals of 15 cm and a depth of 5 cm for planting small pieces of coral.
[0046] (2) Put the 55 cm × 85 cm × 5.5 cm granite culture plate in the circulating water tank, water will flow in from the water outlet to replenish, add water to cover the culture plate for about 50 cm, and install the culture plate on the wall plate at a distance of 20 cm from the water surface. Wave machine, making waves at a frequency of 15 times per minute. Add calcium carbonate and calcium chloride to make the calcium concentration 800ppm, and make the alkalinity 6meq / L, pH 8.5; connect CO 2 Storage tank and gas pump, regulating CO 2 The concentration is 33ppt, the dissolved oxygen is greater than 8mg / L; the ammonia value is guaranteed to be less than 0.1p...
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