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Albibacter sp. zjut528 and application thereof in resolving metalaxyl

A white bacillus, metalaxyl technology, applied in the direction of bacteria, microbe-based methods, microbes, etc.

Inactive Publication Date: 2016-11-16
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the production of (R)-metalaxyl directly by splitting racemic metalaxyl

Method used

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  • Albibacter sp. zjut528 and application thereof in resolving metalaxyl
  • Albibacter sp. zjut528 and application thereof in resolving metalaxyl
  • Albibacter sp. zjut528 and application thereof in resolving metalaxyl

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Screening of Albibacter sp.zjut528 strain

[0027] 1. Enrichment culture:

[0028] Take 5g soil sample and put it in a 250mL conical flask, add 100mL metalaxyl enrichment medium (the initial concentration of metalaxyl is 20mg / L), shake culture (30℃, 150rpm) for 1 week, take 5mL upper turbid liquid in 100mL fresh Continue to culture in the enriched medium for 1 week, repeat the above operation process twice, and the culture inoculated each time is taken from the medium obtained from the previous culture. Take 5mL of the culture solution obtained in the third week in a new metalaxyl enrichment medium, at this time the metalaxyl concentration is increased to 50mg / L, and continue to cultivate for 2 weeks according to the above operation, and then follow the above operation to transfer the metalaxyl. The concentration was increased to 100 mg / L and cultured continuously for 2 weeks. The fermentation broth was analyzed by high pressure liquid chromatography (HPLC) t...

Embodiment 2

[0037] Example 2 Morphological, physiological and biochemical identification of strain zjut528

[0038] The strain zjut528 obtained in Example 1 was cultured in a 30°C constant temperature incubator on a metalaxyl primary screening solid medium plate for 3 days, and the morphological characteristics of the colonies were observed, and Gram staining was performed on them, and the morphological characteristics were observed under a microscope.

[0039] Morphological characteristics of colonies on solid medium plates: The colonies of strain zjut528 on metalaxyl primary screening solid medium plates were all round, with raised surfaces, neat edges, moist, smooth, white, opaque, and colonies over time No obvious color change was seen, such as figure 1 shown.

[0040] Morphological characteristics of the strain after Gram staining: The strain zjut528 was Gram-stained and observed under a microscope, and it was a Gram-negative bacteria with a bulbous shape. like figure 2 shown.

...

Embodiment 3

[0046] Example 3 16S rDNA molecular biology identification of strain zjut528

[0047] The bacterial strain zjut528 obtained in Example 1 was sent to a certain biotechnology company in Shanghai for identification, and the sequence length of 16S rDNA was 1236bp (the nucleotide sequence was shown in SEQ ID NO. The 16S rDNA sequence of the related strains in , and the nucleotide homology alignment and phylogenetic analysis were carried out, and it was found that the similarity of an Albibacter methylovorans strain DSM22840T reached 99%. See the phylogenetic tree of the strain zjut528. Figure 4 shown. Combined with the molecular identification of 16S rDNA and the physiological and biochemical characteristics of the strain zjut528, the strain zjut528 was named as Albibacter sp. zjut528.

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Abstract

The invention discloses albibacter sp. zjut528 and an application thereof in resolving metalaxyl. The strain is preserved in China Center for Type Culture Collection in Wuhan University, China on October 29, 2015, with preservation number of CCTCC NO.M 2015650. The strain can catalyze the asymmetric hydrolysis of the racemic metalaxyl, when an reaction is carried out for 18h, a substrate conversion rate can reach 47.5% and ee (enantiomeric excess of products) reach 99.9%, and a main product is (R)-metalaxyl acid.

Description

(1) Technical field [0001] The invention relates to a strain of Albibacter sp.zjut528 and its application in the field of biocatalysis. The strain has a good effect of chirally selectively resolving metalaxyl. (2) Background technology [0002] With the strengthening of environmental protection concepts and the implementation of sustainable development strategies, high efficiency, low toxicity, high activity and low residues have become an inevitable trend in the development of pesticides. When some chiral compounds interact, different enantiomers often exhibit different activities. Some compounds are highly active for one enantiomer, while the other enantiomer is inefficient or even the opposite. Metalaxyl is a white powdery systemic amide fungicide with protective and therapeutic effects, as well as a chiral pesticide. Studies have shown that the activity of the R-isomer of metalaxyl is 20 to 30 times higher than that of the S-isomer, and the drug effect lasts longer, th...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P41/00C12P13/02C12R1/01
CPCC12N1/20C12P13/02C12P41/00C12N1/205C12R2001/01
Inventor 张朝晖张利坤
Owner ZHEJIANG UNIV OF TECH
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