Check patentability & draft patents in minutes with Patsnap Eureka AI!

Phosphatidylinositol specific phospholipase C gene, carrier, engineering bacteria and application

A technology of phosphatidylinositol and genetically engineered bacteria, applied in the application field of medicine, can solve the problems of high risk, difficult research and development, drug resistance, etc., and achieve the effect of high phospholipase activity and good anti-coccidial effect.

Inactive Publication Date: 2016-11-16
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing prevention and treatment of chicken coccidiosis mainly relies on chemical drugs, but there are deficiencies such as drug resistance and drug residues. If vaccination is used for prevention and control, there are also disadvantages of high risk and difficulty in research and development. Using antibiotics or Chinese herbal medicines, There is also the problem of drug resistance, and the effect is average

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Phosphatidylinositol specific phospholipase C gene, carrier, engineering bacteria and application
  • Phosphatidylinositol specific phospholipase C gene, carrier, engineering bacteria and application
  • Phosphatidylinositol specific phospholipase C gene, carrier, engineering bacteria and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Optimization and synthesis of the target gene

[0031] A piece of Bacillus cereus PI-PLC gene (Genbank: M30809.1) obtained from the NCBI database, according to the codon preference of Escherichia coli, under the premise of keeping the amino acid sequence unchanged, optimized the design and synthesis of PI-PLC The target gene (SEQ ID No.1) was synthesized by Hangzhou Qingke Zixi Biotechnology Co., Ltd.

[0032] 2. Acquisition and identification of target gene fragments

[0033] Specific steps: design primers based on the synthesized known sequences. Forward primer P1: 5'-CCCGGTCTCCCATGGGCTCTAACAAAAACTTATCCTGAAA-3', reverse primer P2: 5'-CCCGGTCTCGAATTCTTATTCTTGATTAAGGATT-3', the underline represents the enzyme cleavage site containing BsaI. The synthetic gene PI-PLC was used as template and p1 / p2 as primers for PCR amplification.

[0034] The PCR amplification system is: 1 μL of template, 1 μL of upstream and downstream primers, 25 μL of 2×super HIFI-MIXⅡ, 20 μL of...

Embodiment 2

[0065] In order to study the effect of bacteria PI-PLC on chicks’ anti-coccidial and weight gain effects, the most pathogenic Eimeria tenella was selected to infect normal chicks, and grouped chicks were given different drug treatments. Observe and record the clinical pathological changes, survival rate, body weight change, cecal lesion and oocyst value of chicks, so as to calculate the anticoccidial index of PI-PLC.

[0066] 1. Experimental group

[0067]

[0068]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a phosphatidylinositol specific phospholipase C gene (the sequence of the gene is as shown in SEQ ID No.1), a carrier, engineering bacteria and application of the phosphatidylinositol specific phospholipase C gene to the preparation of medicine for resisting avian coccidiosis. The phosphatidylinositol specific phospholipase C gene has the advantages that the PI-PLC target gene is optimally designed and synthesized, the heterogeneous-source high expression of the PI-PLC gene in escherichia coli is achieved successfully, recombinant protein shows that phospholipase is high in activity, and the expressed PI-PLC applied to avian-coccidiosis-resisting animal experiments is good in avian coccidiosis resistance.

Description

(1) Technical field [0001] The invention relates to a phosphatidylinositol-specific phospholipase C gene, a carrier, an engineering bacterium, and an application thereof in preparing medicines against chicken coccidia. (2) Background technology [0002] There are many types of phosphatidylinositol-specific phospholipase C (PI-PLC), which exist in bacteria, protozoa, yeast, mold, plants, insects and mammals. There are applications in various fields: in the field of medicine, it is mainly used as a vaccine to prevent infection of various pathogenic bacteria; in the field of food, it is mostly used in bread baking, dairy product processing, health food, etc.; properties, animal feed additives, etc. [0003] The chicken coccidiosis incidence rate is high (50-70%), and the lethality rate is high (50-80%), which seriously endangers the aquaculture industry, and the loss caused by coccidiosis is as high as billions of dollars every year. Existing prevention and treatment of chick...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N9/16C12N15/70C12N1/21A61K38/46A61P33/02C12R1/19
CPCC12N9/16A61K38/00C12Y301/04011
Inventor 刘伟皮雄娥王欣汤先泽
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com