ASCs (Adipose Stem Cells) combined POC-PLA (Polycitrate-Poly Lactic Acid) electrostatic spinning patch complex as well as preparation method and application thereof

A POC-PLA, electrospinning technology, applied in prosthesis, medical science, etc.

Active Publication Date: 2016-11-23
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] There are no literature reports on the research and application of ASCs patch combined with POC-PLA patch complex to repair chest wall defects

Method used

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  • ASCs (Adipose Stem Cells) combined POC-PLA (Polycitrate-Poly Lactic Acid) electrostatic spinning patch complex as well as preparation method and application thereof
  • ASCs (Adipose Stem Cells) combined POC-PLA (Polycitrate-Poly Lactic Acid) electrostatic spinning patch complex as well as preparation method and application thereof
  • ASCs (Adipose Stem Cells) combined POC-PLA (Polycitrate-Poly Lactic Acid) electrostatic spinning patch complex as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1: Isolation and identification of rASCs

[0061] 1. Treatment of adipose tissue: The aseptically obtained adipose tissue was rinsed twice with PBS to remove residual adipose tissue, blood clots, red blood cells, etc. on the surface. Then put the adipose tissue in a 10cm petri dish, add 20ml of PBS solution, and treat it at room temperature for 5-10min, then use ophthalmic scissors and medical tweezers to carefully remove the remaining dermis, connective tissue, blood vessels, etc. Wash the adipose tissue again, then move to a new Petri dish and cut it to a volume of 1mm with plastic scissors 3 Irregular tissue blocks.

[0062] 2. Type I collagenase configuration: Weigh 100mg type I collagenase with an electronic balance, dissolve in 100ml DMEM medium, and sterilize with a 0.22um filter.

[0063] 3. Enzymatic hydrolysis of adipose tissue: add an equal volume of 0.1% type I collagenase to the adipose tissue cut into paste, and digest in a constant temperature w...

Embodiment 2

[0073] Example 2: Establishment of rASCs patch combined with POC-PLA electrospun patch complex

[0074] (1) Preparation of POC-PLA electrospun patch

[0075] The preparation of POC-PLA electrospun patch was completed by Professor Ji Yali and Zhu Lei from the School of Materials, Donghua University. The preparation method is as follows:

[0076] 1. Preparation of prepolymer:

[0077] Add citric acid and 1,8-octanediol into a three-necked round-bottomed flask at a molar ratio of 1:1, heat to 160°C in an oil bath under nitrogen protection, and stir electrically for 10 minutes until the powder is completely melted. Then polymerize for about 40 minutes at 140°C under normal pressure to obtain a prepolymer with a certain molecular weight. Quickly remove the prepolymer from the three-neck flask while it is hot, transfer it to a large beaker, add ethanol (dissolve the prepolymer) and distilled water (precipitate the prepolymer) in sequence, and wash repeatedly several times to compl...

Embodiment 3

[0094] Example 3: rASCs patch combined with POC-PLA electrospun patch complex to repair rabbit full-thickness chest wall defect

[0095] Healthy female New Zealand rabbits were selected, the age of the rabbits was 4-5 months, and the body weight was 2.5-3kg. Build animal models.

[0096] 1. Anesthesia: After grasping and fixing, weigh the rabbit with an electronic scale, and inject 0.3ml / Kg of the prepared 1% pentobarbital sodium solution into the ear vein to anesthetize the rabbit.

[0097] 2. Fixation: Fix the anesthetized rabbit supine on the rabbit dissecting board, fix the limbs on the fixator, fully stretch the rabbit, and expose the whole chest.

[0098]3. Skin preparation: use animal shaving pushers to remove the hair on the chest, up to the lower jaw, down to the costal arch, on both sides to the side chest wall near the joints of the upper limbs, and the right upper limb hair can be removed in the right chest wall surgery area to fully expose the operation Area.

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Abstract

The invention relates to the technical field of tissue engineering and in particular relates to an ASCs (Adipose Stem Cells) combined POC-PLA (Polycitrate-Poly Lactic Acid) electrostatic spinning patch complex, a preparation method thereof and application thereof to preparation of chest wall defect reconstruction materials. The patch complex is composed of a POC-PLA electrostatic spinning patch and an ASCs membrane. An elastomer POC material is used as a main body and PLA with the equal ratio is added; the patch with a 3D (Three Dimensional) nano-pore structure is blended through a electrostatic spinning technology and the patch is compounded with the ASCs membrane to construct the patch complex; the ASCs combined POC-PLA electrostatic spinning patch complex has very good mechanical elasticity and can also be used for promoting the reconstruction of an organism, alleviating adhesion caused by inflammatory reactions and promoting a reconstruction process of a chest wall.

Description

technical field [0001] The invention relates to the technical field of polymer materials and tissue engineering, specifically, a novel adipose stem cells (ASCs) combined with POC-PLA (polycitrate-polylactic acid) electrospun biological patch, A new method for repairing defect tissue, and the research application of the patch and method in repairing full-thickness defects of the chest wall. Background technique [0002] Reconstruction of large-area full-thickness chest wall defects is still a difficult problem for plastic surgery and cardiothoracic surgery. Clinically, such operations often require cardiothoracic surgeons to reconstruct the closed chest cavity and reconstruct bone scaffolds. At the same time, soft tissue defects require flexible use by plastic surgeons flaps for graft repair. At present, the factors affecting the reconstruction effect of chest wall defect still lie in the appropriate selection of materials for chest wall reconstruction. Although titanium pl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/44A61L27/38
CPCA61L27/3834A61L27/44C08L67/00C08L67/04
Inventor 张元政邢新唐炜雅方硕杨超栗颖利
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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