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Method for extracting glutamic acid in waste liquor bacterium dregs of glutamic acid fermentation

An extraction method, glutamic acid technology, applied in the field of extraction of glutamic acid from glutamic acid fermentation waste mash residue, can solve environmental pollution, waste of resources and other problems, and achieve the effect of simple extraction method

Active Publication Date: 2016-11-23
SHANDONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, these glutamic acid fermentation waste mash residues were discharged through wastewater, which not only caused environmental pollution but also wasted resources.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Take 250g of glutamate fermentation waste mash bacteria residue (the dry weight is 50% of the bacteria residue weight), add 600ml of 95% ethanol each time, heat and reflux for 2 times, filter after refluxing for 1 hour each time, and finally obtain the filter residue.

[0025] 2. Add water to the filter residue to 1000ml, under the condition of 20kHz, power 700w, ultrasonic 2s and intermittent 2s for over 0.5h, filter 400ml of filtrate. Repeat the above process a total of 5 times of ultrasound, 5 times of filtrate combined a total of 2000ml.

[0026] 3. The filtrate was vacuum rotary evaporated at 65°C to remove water to obtain 16 g of dry matter.

[0027] 4. Add 32ml of water to the dry matter, add 32ml of 12mol / L HCL, after hydrolysis at room temperature for 4h, add 160ml of methanol and let stand for 1h, the liquid crystallizes, pour out the supernatant, dry the crystals and weigh 1.5g.

[0028] Verification experiment: Take a small amount of crystals dissolved in a solut...

Embodiment 2

[0030] 1. Take 150g of glutamic acid wet bacteria residue (dry weight is 50% of the weight of the bacteria residue), add 400ml of absolute ethanol each time, reflux for 2 times, add 400ml of absolute ethanol each time, reflux for 1h, filter, and then repeat the reflux of the filter residue Once, filter the residue.

[0031] 2. Add water to the filter residue to 600ml, under the conditions of 20kHz, power 600w, ultrasonic 2s and 2s intermittent for 0.5h, filter 240ml filtrate, add water to the filter residue to 600ml, repeat the ultrasonic 5 times with the same method, and combine the 5 filtrates. 1200ml.

[0032] 3. Rotary evaporation of the filtrate at 65° C. to remove water to obtain 10 g of dry matter.

[0033] 4. Add 20ml of water to the dry matter, and then add 20ml of 12mol / L HCL. After hydrolyzing at room temperature for 4 hours, add 100ml of methanol and let it stand for 1 hour. The liquid crystallizes. Pour out the supernatant. Dry the crystals and weigh 1g. The crystallin...

Embodiment 3

[0035] The conditions are the same as in Example 1. The ethanol concentration used for the reflux of bacteria residue is 90%, the power used for ultrasound is 800w, and the prepared dry matter is 17g. Add 34ml of water to the dry matter, and then add 34ml of 12mol / L HCL, and hydrolyze at room temperature for 4h Then, 170 ml of methanol was added and left to stand for 1 hour, the liquid crystallized, the supernatant was poured out, the crystals were dried and weighed to 1.65 g. Contrast detection with glutamic acid standard thin layer chromatography and infrared spectroscopy. The substance is glutamic acid.

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Abstract

The invention discloses a method for extracting glutamic acid in waste liquor bacterium dregs of glutamic acid fermentation. The method specifically includes: adding ethanol into the waste liquor bacterium dregs of glutamic acid fermentation to perform heating reflux filtration so as to obtain filter residues 1, and adding ethanol into the filter residues 1 to repeat the heating reflux filtration so as to obtain filter residues 2; secondly, adding water into the filter residues 2 obtained in the first step to perform ultrasonic treatment for 4-6 times, and filtering after each ultrasonic treatment to obtain filtrate for standby; adding water into the filter residues obtained after the filtration to perform ultrasonic treatment again, filtering, and combining all filtrate after the ultrasonic treatment; thirdly, evaporating the combined filtrate to remove moisture so as to obtain dry substances; fourthly, adding water and hydrochloric acid into the dry substances obtained in the third step to perform hydrolysis, adding methanol after the hydrolysis, standing for crystalizing, and taking out and drying the crystals to obtain the glutamic acid. The method has the advantages that the method is simple, expensive complex equipment is not needed, and the yield of the glutamic acid is 0.5-0.7% of the dry weight of the bacterium dregs.

Description

Technical field [0001] The invention belongs to the field of food, and particularly relates to a method for extracting glutamic acid from glutamate fermentation waste mash. Background technique [0002] my country is a large MSG producer, and MSG production ranks first in the world. At present, there are dozens of large-scale MSG production enterprises in my country, and most of these MSG enterprises use grain and starch as raw materials for fermentation production. Domestically, Brevibacterium glutamicum is mainly used as a fermentation strain to ferment to produce glutamic acid, and then refine MSG. With the production of MSG, a large number of industrial by-products are produced. Glutamic acid fermentation waste mash is the main by-product of the monosodium glutamate industry, with an annual output of up to one million tons. In the past, these glutamic acid fermentation waste mash bacteria residues were discharged through wastewater, which caused environmental pollution and...

Claims

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Application Information

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IPC IPC(8): C07C227/40C07C227/42C07C229/24
CPCC07C227/40C07C227/42C07C229/24
Inventor 刘代成
Owner SHANDONG NORMAL UNIV