A method for identifying in vivo analytical markers of antischizophrenia drugs in nails
A technology of schizophrenia and markers, which is applied in the field of identification, can solve the problems of sample limitations, etc., and achieve the effects of strong selectivity, mild reaction conditions and high precision
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Embodiment 1
[0025] This embodiment provides a method for quickly identifying in vivo analysis markers of anti-schizophrenia drugs in nails. First, put the nails to be detected and the internal standard clozapine-d4 together in an eppendorf centrifuge tube for microwave heating; Add methanol to the obtained mixture, vortex, and perform centrifugation; take the supernatant of the mixture after centrifugation, and perform liquid chromatography-tandem mass spectrometry analysis on the supernatant. Specific steps are as follows:
[0026] 1) Sample pretreatment
[0027] For poison and drug users, the nails must be decontaminated with organic solvents before treatment.
[0028] The pretreatment of nails is carried out according to the following steps: the nail samples are washed three times with 5 mL of deionized water for 3 minutes each time; and then washed twice with 5 mL of acetone solution for 2 minutes each time. The pretreated nails are dried naturally and cut into small pieces for iden...
Embodiment 2
[0043] Use cosmetic nail clippers to cut along the edge of the nail of the patient taking the medicine, store it in a clean and dry kraft paper bag, and store it in the dark at room temperature. Nail samples were washed three times with 5 mL deionized water, 3 minutes each time; then washed twice with 5 mL acetone solution, 2 minutes each time. Let dry at room temperature. Cut pretreated nails into fine pieces. Take the nail fragment sample, accurately weigh 20 mg, place it in an eppendorf tube, add 100 μL of the internal standard clozapine-d4, place it in a microwave oven, and heat it at 700W twice for 3 minutes each time, with an interval of 5 minutes in between to cool the system. Take it out and let it stand still to return to room temperature, add 400 μL of methanol, vortex, and centrifuge at 12000 r / min for 30 s. Take 200 μL of the supernatant and inject directly. The sample was subjected to the above-mentioned liquid chromatography-tandem mass spectrometry analysis, ...
Embodiment 3
[0045] Use cosmetic nail clippers to cut along the edge of the nail of the patient taking the medicine, store it in a clean and dry kraft paper bag, and store it in the dark at room temperature. Nail samples were washed three times with 5 mL deionized water, 3 minutes each time; then washed twice with 5 mL acetone solution, 2 minutes each time. Let dry at room temperature. Cut pretreated nails into fine pieces. Take the nail fragment sample, accurately weigh 20 mg, place it in an eppendorf tube, add 100 μL of the internal standard clozapine-d4, place it in a microwave oven, and heat it at 700W twice for 3 minutes each time, with an interval of 5 minutes in between to cool the system. Take it out and let it stand still to return to room temperature, add 400 μL of methanol, vortex, and centrifuge at 12000 r / min for 30 s. Take 200 μL of the supernatant and inject directly. The sample was subjected to the above liquid chromatography-tandem mass spectrometry analysis, and the ex...
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