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Extraction and purification process for total phenolic acid in chaenomeles speciosa

A technology of wrinkled papaya and total phenolic acids, which is applied to medical preparations containing active ingredients, plant raw materials, plant/algae/fungus/moss components, etc., can solve the problems of low extraction efficiency and waste of total phenolic acids, Achieve the effects of high production efficiency, good extraction rate and simple process steps

Active Publication Date: 2016-12-07
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

The extraction efficiency of total phenolic acid substances contained in wrinkled papaya is low, and failure to make good use of this species resource will cause a certain degree of waste

Method used

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  • Extraction and purification process for total phenolic acid in chaenomeles speciosa
  • Extraction and purification process for total phenolic acid in chaenomeles speciosa

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The method of extraction and purification provided in this embodiment is as figure 1 As shown, in this embodiment, the selection of various process parameters and the selection of resin types have been repeatedly tested in order to obtain the best technical conditions.

[0021] 1) The main characteristics of the macroporous resin used in the experiment

[0022] According to the polarity and other physical parameters of the macroporous resin, the following six types of resins, D101, AB-8, NKA-9, S-8, HPD750, D1400, were screened for pretreatment.

[0023] 2) Static adsorption and desorption test of resin

[0024] Weigh the above-mentioned types of resins respectively, put them in conical flasks, add a certain concentration of phenolic acid extract deionized water solution, seal with a parafilm, place in a constant temperature oscillator, 25 ° C, 120r / min, shake for 24h, After reaching the adsorption equilibrium, filter the filtrate to determine the concentration of phe...

Embodiment 2

[0058] Take about 5g of D101 macroporous resin, pack it into a column, wash it with alcohol and wash it with water until there is no alcohol smell, apply the aqueous solution of wrinkled papaya extract with a concentration of approximately 1.0mg / ml, the flow rate of the sample is 2BV / h, and the sample volume is 20BV, and the effluent is collected S-1, deionized water elution, elution flow rate 2BV / h, dosage 4BV, collected effluent S-2, 60% ethanol solution elution, elution flow rate 4BV / h, dosage 4BV, collected effluent C-3 , respectively measure the phenolic acid concentration of the three effluents to obtain the quality of the effluent C-3. Repeat this experiment three times to obtain the adsorption rate and elution rate, as well as the content of total phenolic acids in papaya wrinkled skin after purification. The conclusions are shown in the table below.

[0059]

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Abstract

The invention provides an extraction and purification process for total phenolic acid in chaenomeles speciosa. The extraction and purification process includes the following steps that the chaenomeles speciosa raw medicinal material is smashed, powder obtained after smashing is subjected to heating reflux for 3-5 h with an ethanol solution, and after filtering, filter liquor is collected; the filter liquor is concentrated to a constant weight, extract is obtained, and the content of total phenolic acid in the extract is measured through the foline-phenol method; the extract is dissolved with deionized water and then prepared into a phenolic acid solution for use; the model of macroporous resin in use is selected, the selected macroporous resin is packed into a column, and after the resin is pretreated, the phenolic acid solution is subjected to dynamic sample loading adsorption; substances on the surface of the resin and part of water-soluble carbohydrates are washed off with deionized water; finally, the resin is eluted with ethyl alcohol, phenolic acid adsorbed in the resin is desorbed, and collected eluate is total phenolic acid in chaenomeles speciosa. According to the extraction and purification method, the process of performing ethanol extraction and then resin purification is adopted, the extraction rate is high, and the extraction and purification method is suitable for industrial production.

Description

technical field [0001] The invention relates to the field of production technology, in particular to a process for extracting, separating and purifying total phenolic acids in wrinkled papaya, which is used in industrial production to increase the content of total phenolic acids. Background technique [0002] Wrinkled skin papaya [Chaenomeles speciosa (Sweet) Nakai] is the fruit of the genus Papaya in the Rosaceae family, also known as the quince, the papaya, and the iron-footed pear. Originated in my country, it is mainly distributed in East China, Central China and Southwest China. Wrinkled papaya contains various active ingredients such as organic acids, flavonoids, oleanolic acid, saponins, superoxide dismutase, etc. [0003] Phenolic acids are a class of phenolic compounds found in fruits, vegetables, and grains. In recent years, a large number of studies have reported that phenolic acids have various biological activities, such as anti-oxidation, anti-tumor, antibact...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/732
Inventor 覃瑞杨光忠肖敏刘虹
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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