Microorganism organic fertilizer and preparation method thereof
A microbial organic fertilizer, biological technology, applied in the preparation of organic fertilizer, biological organic part treatment, organic fertilizer and other directions, can solve the problems of economic loss for growers, unfavorable crop growth, increase planting cost, etc., to enhance resistance, reduce The number of applications, the effect of reducing production costs
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[0034] In the present invention, the preparation method of Paecilomyces fumigatus preferably comprises the following steps:
[0035] 1) activating the Paecilomyces fumigatus species;
[0036] 2) inoculating the activated Paecilomyces fumigatus in the Paecilomyces fumigatus liquid culture medium for liquid fermentation culture, culturing for 40-56 hours to stop fermentation, and obtaining Paecilomyces fumigatus fermentation broth;
[0037] 3) inoculating the Paecilomyces fumigatus fermentation broth obtained in the step 2) into the solid culture medium of Paecilomyces fumigatus, and performing solid fermentation culture to obtain Paecilomyces fumigatus.
[0038] In the present invention, the activation of the Paecilomyces fumigatus species is preferably to inoculate the preserved Paecilomyces fumigatus species in an eggplant-shaped bottle equipped with a nutrient agar medium, and cultivate them at 28-32°C for 25-50 hours. Carry out activation culture.
[0039] After the activ...
Embodiment 1
[0070] Preparation of Paecilomyces fumigatus: Inoculate the preserved Paecilomyces fumigatus strains into eggplant-shaped bottles equipped with nutrient agar medium, and cultivate them at 28°C for 25 hours for activation culture; the Paecilomyces fumigatus after activation culture Mold was inoculated with 20% inoculum in the liquid medium after sterilized at 121°C for 35 minutes, and liquid fermentation was carried out in the fermenter. The specific fermentation conditions were: temperature was 25°C; initial pH value was 6.5; The volume ratio of sterile air to the fermentation liquid is 1:1.5; stop the fermentation after 48 hours of fermentation to obtain the Paecilomyces fumigatus fermentation liquid; inoculate the obtained Paecilomyces fumigatus fermentation liquid on solid In the culture medium, distilled water was added to the solid medium to make the water content of the solid medium 50%, and then solid fermentation culture was carried out. The fermentation culture conditi...
Embodiment 2
[0074]Preparation of Paecilomyces fumigatus: Inoculate the preserved Paecilomyces fumigatus strains into eggplant-shaped bottles equipped with nutrient agar medium, and cultivate them at 25°C for 30 hours for activation culture; the Paecilomyces fumigatus after activation culture Mold was inoculated with 15% inoculum in the liquid medium after sterilized at 121°C for 35 minutes, and liquid fermentation was carried out in the fermenter. The specific fermentation conditions were as follows: temperature was 28°C; pH value was 7.0; The volume ratio of bacterial air to fermentation liquid is 1:1.6; stop the fermentation after 50 hours of fermentation to obtain Paecilomyces fumigatus fermentation liquid; inoculate the obtained Paecilomyces fumigatus fermentation liquid on solid culture with 15% inoculum In the base, add distilled water to the solid medium to make the water content of the solid medium 50%, and then carry out solid fermentation culture. The fermentation culture conditi...
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