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Free fatty acid detection kit and preparation method
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A detection kit and free fatty acid technology, applied in the field of medical detection, can solve the problems of unstable enzyme activity, easy precipitation, affecting detection accuracy, etc.
Active Publication Date: 2019-05-07
HONGKUI BIOLOGICAL CHINA CO LTD
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[0005] In order to solve the problem that the enzyme activity in the free fatty acid determination kit is unstable and easy to precipitate, which affects the detection accuracy, the application discloses a free fatty acid detection kit with stable enzyme activity, low precipitation and high detection accuracy in the kit.
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Embodiment 1
[0038] A free fatty acid detection kit, containing reagent 1 and reagent 2,
[0039] The composition of reagent 1 is as follows:
[0082] The volume ratio of reagent 1 and reagent 2 is 4:1.
[0083] Preparation of reagent 1: Take water, add sodium dihydrogen phosphate, fully dissolve, adjust pH to 7.2 with NaOH, a...
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Abstract
The invention relates to the field of medical detection technology, and especially relates to a detection kit of non-esterified fatty acids. The kit comprises a reagent 1 and a reagent; the reagent 1 comprises sodium dihydrogen phosphate, coenzyme A, adenosine triphosphate, acyl coenzyme A synthetase, MgCl2, and a Trinder substrate, wherein the pH value is adjusted to 7.2; the reagent 2 comprises sodium dihydrogen phosphate, flavin adenine dinucleotide, 4-aminoantipyrine, acyl coenzyme A synthetase, peroxidase, alkylglucoside APG0810, mannitol, EDTA-2Na, and polyoxyethylene dodecyl ether, where the pH value is adjusted to 7.2. Surfactants in the reagents are selected, so that activity of various enzymes is stable and are not easy to precipitate, shelf-life is prolonged, enterprise risk cost is reduced, and enterprise benefits are improved.
Description
technical field [0001] The invention relates to the technical field of medical detection, in particular to a free fatty acid detection kit, and also to a preparation method of the free fatty acid detection kit. Background technique [0002] Free fatty acids, namely non-esterfied fatty acid (NEFA), are intermediate products of fat metabolism in the human body, and are also substrates of cell membrane lipid structures and donors of intracellular signaling molecules such as prostaglandins. When glycogen, the energy source for muscle activity, is exhausted, adipose tissue will decompose neutral fat into free fatty acids for energy use. Although free fatty acids only account for a small part of body fat, they meet a large part of energy demand, so they are important indicators for monitoring body fat metabolism and glucose metabolism. Free fatty acids can not only reflect the body's fat metabolism and blood lipid levels, evaluate blood sugar and assist in the diagnosis of diabet...
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