Unlock instant, AI-driven research and patent intelligence for your innovation.

Method, reagent and kit for quantitatively measuring troponin I in human serum

A technique for quantitative determination of troponin, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of small detection linear range, high price, and long time consumption, and achieve the effect of fully automatic analysis and easy operation

Inactive Publication Date: 2016-12-07
浙江维益生物科技有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have the disadvantages of small detection linear range, cumbersome operation, long time-consuming, expensive, excessive waste of resources, and are not suitable for routine inspection, especially large-scale epidemiological inspection or simultaneous detection of many items in clinical large-scale specimens

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method, reagent and kit for quantitatively measuring troponin I in human serum
  • Method, reagent and kit for quantitatively measuring troponin I in human serum
  • Method, reagent and kit for quantitatively measuring troponin I in human serum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Configure the following reagent I and reagent II of the present invention according to the following ingredients and ratios:

[0022] Reagent I:

[0023] Glycine buffer 140mmol / L

[0024] Polyethylene glycol 6000 2.4%

[0025] Reagent II:

[0026]

[0027] Mix 225 μl reagent I and 25 μl serum sample in a sample tube, incubate at 37°C for 5 minutes, use the Abbott C16000 automatic biochemical analyzer, measure the absorbance A1 at the dominant wavelength of 500 nm, then add 70 μl reagent II to the sample, mix Mix well, incubate at 37°C for 5 minutes, and measure the absorbance A2 at the same wavelength. Calculate the ΔA sample according to the following formula (1). Use the same method and conditions to measure the absorbance value of the standard solution, and calculate the △A standard. Then calculate troponin I content in the serum sample of sample according to formula (2): △ A=A2-A1 (1)

[0028] Troponin I content in serum (ng / mL) = △A sample / △A standard × st...

Embodiment 2

[0031] Configure the following reagent I and reagent II of the present invention according to the following ingredients and ratios:

[0032] Reagent I:

[0033] Glycine buffer 110mmol / L

[0034] Polyethylene glycol 6000 1.8%

[0035] Reagent II:

[0036]

[0037] Mix 225 μl of reagent I and 25 μl of serum sample in a sample tube, incubate at 37°C for 5 minutes, use a Hitachi 7180 automatic biochemical analyzer, measure the absorbance A1 at a dominant wavelength of 500 nm, and then add 70 μl of reagent II to the sample, Mix well, incubate at 37°C for 5 minutes, and measure the absorbance A2 at the same wavelength. Calculate the ΔA sample according to the following formula (1). Use the same method and conditions to measure the absorbance value of the standard solution, and calculate the △A standard. Then calculate troponin I content in the serum sample of sample according to formula (2): △ A=A2-A1 (1)

[0038] Troponin I content in serum (ng / mL) = △A sample / △A standard ...

Embodiment 3

[0040] Configure the following reagent I and reagent II of the present invention according to the following ingredients and ratios:

[0041] Reagent I:

[0042] Glycine buffer 180mmol / L

[0043] Polyethylene glycol 6000 2.6%

[0044] Reagent II:

[0045]

[0046]

[0047] Mix 225 μl reagent I and 25 μl serum sample in the sample tube, incubate at 37°C for 5 minutes, use the Olympus AU400 automatic biochemical analyzer, measure the absorbance A1 at the main wavelength of 500 nm, and then add 70 μl to the sample Reagent II, mix well, incubate at 37°C for 5 minutes, measure the absorbance A2 at the same wavelength. Calculate the ΔA sample according to the following formula (1). Use the same method and conditions to measure the absorbance value of the standard solution, and calculate the △A standard. Then calculate the troponin I content in the serum sample of the sample according to formula (2):

[0048] △A=A2-A1 (1)

[0049] Troponin I content in serum (ng / mL) = △A ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a reagent for quantitatively measuring troponin I content in human serum. The reagent comprises a reagent I and a reagent II which are respectively placed, wherein the reagent I comprises glycine buffer solution and polyethylene glycol 6000; the reagent II comprises glycine buffer solution, goat-anti-human troponin I antibody, latex, tween-20, protein protector 2 and parvalbumin. A method and kit for quantitatively measuring the troponin I in the human serum only needs several dozen microliters of serum, does not need separation treatment such as centrifuging or electrophoresis, and is simple to operate, capable of satisfying the requirements of full-automatic analysis and applicable to timely and accurate measuring of large-scale samples.

Description

technical field [0001] The application relates to a quantitative determination method, reagent and kit for troponin I in human serum. Background technique [0002] Troponin I, composed of three subunits of troponin T (cTnT), troponin I (cTnI) and troponin C (cTnC), together with tropomyosin regulates the calcium ion on striated actin ATP Enzyme activity to regulate actin and myosin interactions. After myocardial injury, the cardiac troponin I complex is released into the blood, and after 4-6 hours, it begins to increase in the blood, and the elevated troponin I can remain in the blood for a long time of 6-10 days. Troponin I has high myocardial specificity and sensitivity, so troponin I has become the most ideal marker of myocardial infarction. [0003] Currently known methods for the determination of troponin include electrochemiluminescence immunoassay; enzyme-linked fluorescence assay. These methods have the disadvantages of small detection linear range, cumbersome ope...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6893G01N33/6887G01N2333/4712G01N2800/325
Inventor 张洋永
Owner 浙江维益生物科技有限公司