A method for producing ginger mycorrhizal tissue culture seedlings
A technology for rooting culture and tissue culture seedlings, applied in the directions of horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as restricting the development of ginger industry, and achieve the effects of promoting ginger growth, good ginger growth, and ginger growth promotion.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0039] The screening process of a Glomus mosei FCGX-1631 is as follows:
[0040] (1) Put 10-20 g of perennial root sugarcane rhizosphere soil collected from Fusui County, Guangxi, into a food mixer cup as a culture, add 600 mL of deionized water and centrifuge at high speed for 3-5 s;
[0041] (2) Pour out the material obtained by high-speed centrifugation in step (1), and pass through three soil standard sieves (the aperture is 0.8mm in the upper layer, 0.25mm in the middle, and 0.0385mm in the lower layer), and most of the gravel remains in the food mixer cup. Rinse each layer of sieve with running water until the water flowing out is clear;
[0042] (3) The residue in the lower sieve is transferred to a centrifuge tube of 60% sucrose, centrifuged at 1500 rpm for 3 min, and the supernatant in the centrifuge tube is quickly poured into a sieve with an aperture of 0.0385 mm;
[0043] (4) Rinse the supernatant residue in the sieve with water for 1 to 2 minutes and then transfe...
Embodiment 2
[0055] Promoting Effects of Bacterial Strains and Inoculants on Planting of Potted Ginger
[0056] 1. Preparation of bacterial strains
[0057] The preparation method of a Glomus mosesii FCGX-1631 bacterial agent is as follows:
[0058] (a) Using corn as the host, inoculate the root system of corn seedlings with the spores of Glomus mosesii FCGX-1631, and plant them in 121°C under high-pressure steam for 1 hour of sterilization, river sand: zeolite = 3:1 volume ratio river sand zeolite culture In the substrate, cultured under light for 12-16 weeks to obtain Glomus mosesi hyphae; during the light culture period, 100 mL of Hoagland nutrient solution with a mass concentration of 25-50% was poured every 7-10 days; among them, potted plants (780×350× 250mm) production mode, the host density is 35-45 plants / pot;
[0059] (b) Stop watering the corn cultivated in step (a) for 12 to 16 weeks, remove the stalk after the plant dries up, and keep the root system, and the hyphae obtained...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More