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A preparation method of cross-linked antibacterial acellular matrix material

An acellular matrix and amino acid technology, applied in the field of preparation of antibacterial acellular matrix materials, to achieve the effects of good controllability, reduced antigenicity, and high stability

Active Publication Date: 2019-09-13
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current methods often only satisfy one of the

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Weighing 100 parts by weight of acellular porcine dermal matrix and adding it to the reactor;

[0032] (2) Add 130 parts by weight of boric acid-borax buffer solution with a pH value of 8.5, and rotate for 30 minutes;

[0033] (3) Take by weighing 8 parts by weight of oxidized N-hydroxypropyltrimethylammonium chloride chitosan with a degree of oxidation of 8%, and dissolve it in 20 parts by weight of distilled water;

[0034] (4) Add the aqueous solution of oxidized chitosan quaternary ammonium salt into the reactor, raise the temperature to 37°C, react for 16 hours, and pour off the reaction waste liquid;

[0035] (5) Add 300 parts by weight of pure water, wash for 20 minutes, and pour off the waste liquid;

[0036] (6) Add 100 parts by weight of boric acid-borax buffer solution with a pH value of 8.5 at 37°C, add 1.5 parts by weight of lysine, rotate for 2 hours, and pour off the waste liquid;

[0037] (7) Add 1000 parts by weight of pure water, wash for 30 minu...

Embodiment 2

[0041] (1) Weighing 100 parts by weight of bovine pericardium decellularized matrix and adding it to the reactor;

[0042] (2) Add 200 parts by weight of acetic acid-hydrochloric acid buffer solution with a pH value of 4.8, and rotate for 60 minutes;

[0043] (3) Weigh 4 parts by weight of oxidized N, N, N-trimethyl chitosan quaternary ammonium salt with an oxidation degree of 80%, and dissolve it in 50 parts by weight of acetic acid-hydrochloric acid buffer solution with a pH value of 4.8;

[0044] (4) Add the solution of oxidized chitosan quaternary ammonium salt into the reactor, raise the temperature to 42°C, rotate for 8 hours, and pour off the reaction waste liquid;

[0045] (5) Add 500 parts by weight of pure water, rotate for 20 minutes, and pour off the waste liquid;

[0046] (6) Add 150 parts by weight of acetic acid-hydrochloric acid buffer solution with a pH value of 4.8, add 0.5 parts by weight of arginine, rotate for 4 hours at 42°C, and pour off the waste liqui...

Embodiment 3

[0051] (1) Weighing 100 parts by weight of amniotic membrane decellularized matrix and adding it to the reactor;

[0052] (2) Add 100 parts by weight of NaHCO with a pH value of 9.4 3 -NaOH buffer solution, rotate for 20 minutes;

[0053] (3) Weigh 16 parts by weight of oxidized O-hydroxypropyltrimethylammonium chloride N-trimethyl chitosan with a degree of oxidation of 5%, and dissolve it in 50 parts by weight of pure water;

[0054] (4) Add the solution of oxidized chitosan quaternary ammonium salt into the reactor, raise the temperature to 32°C, rotate for 24 hours, and pour off the reaction waste liquid;

[0055] (5) Add 800 parts by weight of pure water, rotate for 40 minutes, and pour off the waste liquid;

[0056] (6) Add 150 parts by weight of NaHCO with a pH value of 9.4 3 -NaOH buffer solution, adding 2.0 parts by weight of glycine, reacting at 32°C for 1 hour, pouring off the waste liquid;

[0057] (7) Add 300 parts by weight of pure water, rotate for 15 minutes...

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Abstract

The invention discloses a preparation method of a cross-linking antibacterial acellular matrix material. Oxidized chitosan quaternary ammonium salt is cross-linked with an acellular matrix material, multiple aldehyde groups on oxidized chitosan quaternary ammonium salt are cross-linked with active groups on the acellular matrix, the structural stability of the material is improved, meanwhile, chitosan quaternary ammonium salt is grafted in the acellular matrix material, and therefore excellent antibacterial and bacteriostatic performance is provided for the acellular matrix material. According to the method, cross-linking and providing of the antibacterial performance of the material are combined, the preparation method is a preparation method of the novel functional acellular matrix material, and the method can be widely applied to the field of biological materials.

Description

technical field [0001] The invention relates to a preparation method of an antibacterial decellularized matrix material, which is applied in the field of biological materials. Background technique [0002] The decellularized matrix material is obtained by treating the matrix material with physical, chemical and biological methods, performing decellularization treatment, and removing related antigens. It removes the cellular components in natural materials, retains matrix components, effectively reduces the immunogenicity of natural materials, and at the same time maintains the basic structure and properties of matrix materials (such as macroscopic weaving structure, microscopic pore structure, physical and mechanical strength, etc.) . Common acellular matrix materials include acellular dermal matrix, acellular corneal stroma, amniotic membrane acellular matrix, porcine aortic acellular matrix, bovine (or porcine) pericardial acellular matrix, porcine small intestinal submuc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/20A61L27/54A61L27/58
Inventor 但年华但卫华陈一宁
Owner SICHUAN UNIV
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