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Method for co-producing glucose and dietary fibers from multiple high-solid enzymes of sweet potato residues through synergetic enzymatic hydrolysis

A technology for dietary fiber and sweet potato dregs, applied in food science, fermentation and other directions, can solve the problems of low-end, short industrial chain and low-value deep processing technology of sweet potato dregs, achieve high value-added resource utilization and simplify units Operation, the effect of saving equipment cost

Inactive Publication Date: 2016-12-14
INST OF PROCESS ENG CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This invention will help solve the problems of low-end deep processing technology of sweet potato dregs, single and low-value products, and short industrial chain, and provide technical reference for my country's sweet potato dregs and even sweet potato deep processing industry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Remove impurities such as soil and fine stones from the sweet potato dregs, and crush them to 40 mesh; add water to the crushed sweet potato dregs to adjust the solid-liquid ratio to 1:2.5 (w / v), add 20 U / g substrate acid cellulase and 5 U / g substrate Pectinase, after stirring evenly, oscillating enzymolysis at 150 rpm at 50°C for 16 hours at natural pH value to obtain a pre-enzymolysis solution; adjust the pH of the pre-enzymolysis solution to about 5.5, and add 20U / g substrate α-amylase , liquefied at 90°C for 2 hours, after cooling the liquefied solution, add 60 U / g substrate glucoamylase, and saccharify at 60°C for 4 hours to obtain a saccharified solution;

[0028] The enzymatic hydrolysis and saccharification liquid of sweet potato dregs after saccharification was centrifuged at 4000rpm, and 7.5% (w / w) powdered activated carbon and diatomaceous earth mixed at a ratio of 1:1 (w / w) were added to the obtained liquid crude sugar liquid, and the mixture was heated at 50...

Embodiment 2

[0031]Remove impurities such as soil and fine stones from sweet potato dregs, and crush them to 10 meshes; add water to the crushed sweet potato dregs to adjust the solid-liquid ratio to 1:3 (w / v), add 30 U / g substrate acid cellulase and 10 U / g substrate Pectinase, stirred evenly, placed in a drum reactor at natural pH value, and enzymatically hydrolyzed at 50°C for 24 hours to obtain a pre-enzymolyzed solution; adjust the pH of the pre-enzymolyzed solution to about 5.0, and add 40U / g at the end The α-amylase of the substrate was liquefied at 85°C for 3 hours. After the liquefied liquid was cooled, 40 U / g of substrate glucoamylase was added, and saccharified at 60°C for 16 hours to obtain a saccharified liquid;

[0032] After saccharification, the enzymatic hydrolysis and saccharification liquid of sweet potato dregs is suction-filtered, and the filtrate is crude sugar liquid, which is mixed with 10% (w / w) powdered activated carbon and diatomaceous earth at a ratio of 1:1 (w / w)...

Embodiment 3

[0035] Remove impurities such as soil and fine stones from the sweet potato dregs, and crush them to 20 meshes; add water to the crushed sweet potato dregs to adjust the solid-liquid ratio to 1:2.5 (w / v), add 40U / g substrate acid cellulase and 10U / g substrate Pectinase, stirred evenly, placed in a drum reactor at the natural pH value, and enzymatically hydrolyzed at 50°C for 12 hours to obtain a pre-enzymolyzed solution; adjust the pH of the pre-enzymolyzed solution to about 6.0, and add 20U / g at the end The α-amylase of the substrate was liquefied at 90°C for 2 hours. After the liquefied solution was cooled, 100 U / g of substrate glucoamylase was added and saccharified at 60°C for 24 hours to obtain a saccharified solution;

[0036] After saccharification, the enzymatic hydrolysis and saccharification liquid of sweet potato dregs was filtered by plate and frame filter, and 12.5% ​​(w / w) of powdery activated carbon and diatomite mixture mixed with 1:1 (w / w) were added to the fil...

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Abstract

The invention provides a method for co-producing glucose and dietary fibers from multiple high-solid enzymes of sweet potato residues through synergetic enzymatic hydrolysis, and aims to provide a coupling integrated technology for co-producing high-concentration glucose and high-protein-content dietary fibers from the multiple high-solid enzymes of the sweet potato residues through synergetic enzymatic hydrolysis. The method comprises the steps of impurity removal and smashing of the sweet potato residues, pre-enzymatic hydrolysis of high solids of the sweet potato residues, liquefaction and sugaring of pre-enzymatic hydrolysate, solid-liquid separation of the enzymatic hydrolysate, preparation of a glucose solution from the enzymatic hydrolysate and preparation of the dietary fibers from enzymatic hydrolysis residues. According to the technical scheme provided by the invention, high-solid enzymatic hydrolysis with the sweet potato residue substrate content of 20 to 28.6 percent can be realized; the substrate conversion rate from the sweet potato residues into the glucose can be over 65 percent, and the high-purity sweet potato residue dietary fibers can be obtained. The method can assist in solving the problems that the deep processing technology for the sweet potato residues is in a low grade, produces single and low-value products, is short in industrial chain and the like, and a technical reference is supplied to the deep processing industries for the sweet potato residues, and even sweet potatoes, in China.

Description

【Technical field】 [0001] The invention belongs to the field of resource utilization of agricultural product processing waste, and in particular relates to a method for synergistic enzymolysis and joint production of glucose and dietary fiber of sweet potato dregs with high solid phase and multiple enzymes. 【Background technique】 [0002] Sweet potato is nutritionally balanced and complete, and its health care function is excellent. It is a nutritious food and health food recognized by the world. important raw material. At present, the total output of sweet potatoes in my country remains at about 100 million t / year, accounting for 80% of the world's total. And sweet potato residue is the residue after sweet potato flour factory produces sweet potato flour and sweet potato starch factory extracts sweet potato starch, accounts for 45%~60% of fresh weight. Sweet potato dregs generally come from sweet potato food processing plants, which are the waste dregs after sweet potato e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/02A23L33/21
CPCC12P19/14C12P19/02C12P2201/00
Inventor 韩业君邱卫华
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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