Preservation solution for cervical cells
A cervical cell and preservation solution technology, applied in the preservation, application, and animal husbandry of human or animal bodies, can solve the problems of easy misjudgment of patient health, cumbersome processing, and insufficient suction of samples, and achieve easy preparation and improved The effect of detection efficiency and cheap raw materials
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experiment example 1
[0022] The effect of the mass fraction of dodecyltrimethylammonium chloride in the cervical cell preservation solution on the crushing effect of red blood cells and the detection results of HPV was tested:
[0023] The experimental process is as follows: dodecyltrimethylammonium chloride is mixed into PBS buffer solution to form the primary storage solution; PBS buffer solution is phosphate buffer solution with pH=7.4 containing 0.05% Tween-20.
[0024] Put the initial preservation solutions containing different mass percentages of dodecyltrimethylammonium chloride into different reaction containers and clearly mark them, and take samples from the same cervical cell sample and put them into their respective reaction containers. After the reaction, record the reaction results in the table below;
[0025] Table 1:
[0026]
[0027] The reaction results are shown in table 1: when the mass percent of dodecyltrimethylammonium chloride in the initial storage solution is 5%, the ...
experiment example 2
[0029] In the same volume of primary preservation solution containing 10% dodecyltrimethylammonium chloride by mass percentage, different masses of proteinase K were added respectively to form finished preservation solutions with different mass-volume ratio concentrations to the treatment of mucus Effect and impact on HPV test results.
[0030] The experimental process is as follows: In the same volume of primary preservation solution containing 10% dodecyltrimethylammonium chloride by mass percentage, different masses of proteinase K are added respectively to form the mass-volume ratio concentration of proteinase K and the primary preservation solution. Different storage solutions for multiple finished products should be clearly marked in different reaction containers, and samples should be taken from the same sample and placed in their respective reaction containers. After the reaction is carried out under the same conditions, record the reaction results as shown in Table 2; ...
experiment example 4
[0038] Experimented cervical cell preservation solution of the present invention (mass percentage of dodecyltrimethylammonium chloride in preservation solution for the first time is 10%, the mass volume ratio concentration of proteinase K and preservation solution for the first time is 5mg / ml) in different In the case of elution, the cervical cell preservation solution of the manufacturer of the prior art (the cell preservation solution produced by Chaozhou Kaipu Biochemical Co., Ltd., main component: PBS) produced in the fluorescent quantitative PCR technology verification experiment under the condition of elution Is there any difference between the curve and CT value;
[0039] Before the experiment, HPV.16 is required + PCR amplification to obtain a standard curve, such as figure 1 shown.
[0040] During the experiment, 5 cases of cervical cell bloody samples were used to respectively adopt the non-eluting operation of the present invention and the elution operation of the...
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