A kind of cervical cell preservation solution
A cervical cell and preservation solution technology, applied in the preservation, application, and animal husbandry of human or animal bodies, can solve the problems of easy misjudgment of patient health, cumbersome processing, and insufficient suction of samples, and achieve easy preparation and improved The effect of detection efficiency and cheap raw materials
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
experiment example 1
[0022] The effect of the mass fraction of dodecyltrimethylammonium chloride in the cervical cell preservation solution on the red blood cell crushing effect and the HPV detection result was tested:
[0023] The experimental process is as follows: dodecyltrimethylammonium chloride is mixed with PBS buffer to form the primary preservation solution; the PBS buffer is a phosphate buffer with 0.05% Tween-20 and pH=7.4.
[0024] Put the initial preservation solutions containing different mass percentages of dodecyltrimethylammonium chloride in different reaction containers and clearly mark them, and place samples from the same cervical cell sample into their respective reaction containers. Under the same conditions After the reaction, record the reaction results as shown in the table below;
[0025] Table 1:
[0026]
[0027] The results of the reaction are shown in Table 1: When the mass percentage of dodecyltrimethylammonium chloride in the primary storage solution is 5%, the hemolysis ef...
experiment example 2
[0029] Experiments were conducted on the treatment of mucus by adding different masses of proteinase K to the same volume of primary preservation solution containing 10% by mass dodecyltrimethylammonium chloride to form finished preservation solutions with different mass-volume ratios. Effect and impact on HPV test results.
[0030] The experimental process is as follows: in the same volume of the primary storage solution containing 10% by mass dodecyltrimethylammonium chloride, add different masses of proteinase K to form the mass-volume ratio of proteinase K to the primary storage solution Different storage solutions of multiple finished products are clearly marked in different reaction containers, samples are taken from the same sample and put into their respective reaction containers, and after the reaction is carried out under the same conditions, the reaction results are recorded as shown in Table 2;
[0031] Table 2:
[0032] The mass volume ratio of proteinase K
Treatme...
experiment example 4
[0038] The cervical cell preservation solution of the present invention (the mass percentage of dodecyltrimethylammonium chloride in the initial preservation solution is 10%, and the mass-volume ratio of proteinase K to the initial preservation solution is 5 mg / ml) was tested. In the case of elution, compare with the cervical cell preservation solution of the prior art manufacturer (the cell preservation solution produced by Chaozhou Kaipu Biochemical Co., Ltd., the main component: PBS). In the case of elution, the fluorescent quantitative PCR technology verification experiment produced Whether the curve and CT value are different;
[0039] Before the experiment, HPV.16 is required + PCR amplification, get the standard curve, such as figure 1 Shown.
[0040] In the experiment, five blood samples of cervical cells were used, respectively, using the non-eluting operation of the present invention and the elution of the manufacturer’s cell preservation solution. The fluorescent quantit...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More