Hypecoum leptocarpum effective part as well as preparation method and application thereof

A technology of carotid fennel and effective parts, applied in the field of plant extracts, can solve problems such as research and application that have not yet been seen, and achieve the effects of convenient extraction, good anti-tumor effect, and improved extraction efficiency

Inactive Publication Date: 2016-12-21
QINGHAI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research and application on the inhibition of HDAC1 enzyme activity of fennel

Method used

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  • Hypecoum leptocarpum effective part as well as preparation method and application thereof
  • Hypecoum leptocarpum effective part as well as preparation method and application thereof
  • Hypecoum leptocarpum effective part as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiments 1 to 3 are specific implementations of the effective fraction of fennel fennel and its preparation method and application of the present invention, wherein embodiment 1 is the best embodiment.

[0024] Example 1

[0025] The preparation method comprises the following steps: pulverize the fennel fennel, use an ethanol solution with a mass percentage concentration of 75% to 85% for ultrasonic extraction for 1 hour, and filter to obtain an ultrasonic extraction solution and filter residue, and add ethanol with a mass percentage concentration of 75% to 85% to the filter residue The solution was refluxed and extracted 3 times, each time for 0.5 hours, to obtain a refluxed extract, combined with the ultrasonic extract and the refluxed extract, concentrated and dried to obtain an extract; take the extract and disperse it with distilled water to obtain an extract dispersion, and sequentially use petroleum ether, acetic acid Ethyl ester and n-butanol are used as solv...

Embodiment 2

[0027] The preparation method comprises the following steps: pulverize the fennel fennel, use an ethanol solution with a mass percentage concentration of 85% to 95% for ultrasonic extraction for 3 hours, filter to obtain an ultrasonic extraction solution and filter residue, and add ethanol with a mass percentage concentration of 85% to 95% to the filter residue The solution was refluxed twice, each time for 1 hour, to obtain a reflux extract, combined with the ultrasonic extraction and reflux extract, concentrated and dried to obtain an extract; take the extract and disperse it with distilled water to obtain an extract dispersion, which was sequentially washed with petroleum ether, acetic acid Ethyl ester and n-butanol are used as solvents to extract the extract dispersion, and the obtained n-butanol extract is evaporated to obtain the n-butanol part of fennel fennel, and the extracted extract dispersion is concentrated and dried to obtain fennel fennel Aqueous phase extraction...

Embodiment 3

[0029] The preparation method comprises the following steps: pulverize the fennel fennel, use an ethanol solution with a mass percentage concentration of 65% to 75% for ultrasonic extraction for 0.5 hours, and filter to obtain an ultrasonic extraction solution and filter residue, and add ethanol with a mass percentage concentration of 65% to 75% to the filter residue The solution was refluxed twice, each time for 2 hours, to obtain a reflux extract, combined with the ultrasonic extraction and reflux extract, concentrated and dried to obtain an extract; take the extract and disperse it with distilled water to obtain an extract dispersion, which was sequentially washed with petroleum ether, acetic acid Ethyl ester and n-butanol are used as solvents to extract the extract dispersion, and the obtained n-butanol extract is evaporated to obtain the n-butanol part of fennel fennel, and the extracted extract dispersion is concentrated and dried to obtain fennel fennel Aqueous phase ext...

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Abstract

The invention discloses a hypecoum leptocarpum effective part as well as a preparation method and application thereof, belonging to the technical field of plant extract. The hypecoum leptocarpum effective part is selected from one or mixture of over two of a hypecoum leptocarpum n-butyl alcohol part, a hypecoum leptocarpum water-phase extraction part and a hypecoum leptocarpum chloroform part in any mass ratio. The preparation method comprises the following steps: grinding the hypecoum leptocarpum, adding a 65-95% ethanol solution by mass and performing reflux extraction; concentrating and drying to obtain extract; adding distilled water into the extract for dispersion; sequentially extracting with petroleum ether, ethyl acetate and n-butyl alcohol; volatilizing to remove solvents to obtain the hypecoum leptocarpum n-butyl alcohol part; concentrating and drying the extract dispersion liquid after extraction to obtain the hypecoum leptocarpum water-phase extraction part; and adding a 2% hydrochloric acid aqueous solution by mass into the extract for extraction, adjusting the pH value to 9-10, extracting repeatedly with chloroform, and volatilizing to remove solvents to obtain the hypecoum leptocarpum chloroform part. The application of the hypecoum leptocarpum effective part disclosed by the invention in preparing medicines for inhibiting HDAC1 enzymes can be used for preparing anti-tumor medicines.

Description

technical field [0001] The effective part of fennel fennel and its preparation method and application belong to the technical field of plant extracts. Background technique [0002] Hypecoum leptocarpum Hook. f.et Thoms. is an annual herbaceous plant of the family Papaveraceae Hypecoum leptocarpum. It grows on hillsides, grasslands, valleys, river beaches, gravel slopes, sandy textures, etc. at an altitude of 2700-5000 meters, and is mainly distributed in Tibet. , Qinghai, Sichuan, Gansu, Shaanxi, Hebei and other places. Tibetan medicine is called "Barbada", bitter in taste and cool in nature, clearing blood heat, warm heat and toxic heat [See: Northwest Plateau Institute of Biology, Chinese Academy of Sciences. Tibetan Medicine [M]. Xining: Qinghai People's Publishing House, 1991. ], has antipyretic, analgesic, anti-inflammatory and detoxifying effects, can cure colds, headaches, pain in limbs and joints, cholecystitis, and food poisoning; fennel contains a variety of alkal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/66A61P35/00
CPCA61K36/66A61K2236/333A61K2236/39A61K2236/51A61K2236/53
Inventor 梁永欣林鹏程吴疆慈冰李元清曾擎屹高世宝
Owner QINGHAI UNIV FOR NATITIES
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