Chicken inclusion body hepatitis inactivated vaccine and preparation method thereof

An inactivated vaccine and inclusion body technology, which is applied to the field of chicken inclusion body hepatitis inactivated vaccine and its preparation, can solve the problem that chicken inclusion body hepatitis cannot be prevented by vaccines, etc., and reduce the infection rate of bacterial diseases such as Escherichia coli and increase the The immune function of the body, the effect of strong disease resistance

Inactive Publication Date: 2016-12-21
CHONGQING SANJIE ZHONGXIN BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the problem that the existing chicken inclusion body hepatitis cannot be prevented by vaccines, the present invention proposes a chic...

Method used

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  • Chicken inclusion body hepatitis inactivated vaccine and preparation method thereof
  • Chicken inclusion body hepatitis inactivated vaccine and preparation method thereof
  • Chicken inclusion body hepatitis inactivated vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Antigen preparation

[0029] 1.1 Inoculation Dilute the seed poison 15,000 times with sterile saline, inoculate 7-day-old SPF chicken embryos through the yolk sac, 0.1ml per embryo, seal the holes with wax, and incubate at 37°C.

[0030] 1.2 Incubation and observation Discard the chicken embryos that died 48 hours ago, and light the eggs every 6 hours thereafter, collect the chicken embryos that died within 48-120 hours, and refrigerate them at 2-8°C for 12-24 hours.

[0031] 1.3 Harvest Chicken embryos were disinfected with tincture of iodine to disinfect the air cells on the surface of the eggshells, remove the eggshells from the air cells by aseptic operation, and harvest allantoic fluid and embryo bodies. Among them, the allantoic fluid is placed in a sterilized 500ml glass bottle with a capacity of 450ml / bottle, labeled as chicken inclusion body hepatitis virus SDJ15-8 strain E8, and stored at -20°C or below; the embryo body is mashed with tissue Machine crushe...

Embodiment 2

[0058] 1. Experiment on 5-day-old chicks

[0059] 1.1 Method

[0060] 140 5-day-old AA broiler chickens were divided into 7 groups, 20 in each group. Groups 1-6 were injected intramuscularly with chicken inclusion body hepatitis transfer factor adjuvant inactivated vaccine 1-6, 0.3ml / bird. Group 7 was The control group received no treatment. Fifteen days after immunization, intramuscularly inoculate chicken inclusion body hepatitis virus SDJ15-8 strain 100LD50 per chick in groups 1-7, observe for 10 days after virus inoculation, and count the morbidity and mortality of chicks in each group.

[0061] 1.2 Results

[0062] When the virus content of the antigen before inactivation of the vaccine is ≥10-6.0ELD50 / 0.1ml, and the polypeptide content is 2mg / ml, the chicks can be 100% protected. The specific results are shown in Table 1.

[0063] Table 1 The incidence rate and protection rate of chicks in each group

[0064] group

1

2

3

4

5

6

7

I...

Embodiment 3

[0077] Experiment of the earliest time to produce protective antibody after immunizing chickens of different ages with chicken inclusion body hepatitis virus inactivated vaccine

[0078] 15-day-old chick test

[0079] 1.1 Method

[0080] 120 5-day-old AA broiler chicks were divided into 12 groups with 10 birds in each group. Groups 1-6 were immunized with chicken inclusion body hepatitis virus serum type 4 transfer factor adjuvant inactivated vaccine 0.3ml / piece by intramuscular injection, 7- Group 12 served as the control group without any treatment. At 8, 9, 10, 11, 12, and 13 days after immunization, treat all chicks in groups 1 and 7, groups 2 and 8, groups 3 and 9, groups 4 and 10, groups 5 and 11, groups 6 and 12, respectively Intramuscular Inoculation of Chicken Inclusion Body Hepatitis Virus SDJ15-8 Strain 100LD 50 / Only. The above-mentioned groups were observed for 10 days after being inoculated with the virus, and the morbidity and mortality of chicks in each gro...

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Abstract

The invention discloses a chicken inclusion body hepatitis inactivated vaccine and a preparation method thereof. The virus is avian adenovirus group I serotype-4 virus named as SDJ15-8 with the preservation number of CCTCC NO: V201636 and is preserved in the China Center for Type Culture Collection in Wuhan University. The preparation method comprises the following steps: 1) inoculation: diluting the chicken inclusion body hepatitis virus with sterilized normal saline and then inoculating the diluted chicken inclusion body hepatitis virus into SPF chicken embryos through the yolk sac, standing and hatching at 37 DEG C, collecting chicken embryos after death within 48 to 120 hours, harvesting allantoic fluids and embryoid bodies, and crushing the embryoid bodies and then diluting with normal saline to obtain embryoid body fluids; 2) inactivation: uniformly mixing allantoic fluids embryoid body fluids which are qualified after tests according to a volume ratio of 2:1 to obtain mixed chicken embryo liquids, inactivating the mixed chicken embryo liquids with an inactivator and then detecting after inactivation; and 3) vaccine preparation: mixing the inactivated mixed chicken embryo liquids which are qualified after tests in step 2) with a transfer factor, and finally adding sterilized normal saline. The vaccine prepared from the virus and the transfer factor can effectively prevent and control diseases caused by the virus.

Description

technical field [0001] The invention belongs to the field of vaccine preparation, and in particular relates to an inactivated chicken inclusion body hepatitis vaccine and a preparation method thereof. Background technique [0002] Chicken inclusion body hepatitis is characterized by a sudden increase in the death of sick chickens, severe anemia, jaundice, hepatomegaly, hemorrhage and necrosis, and pericardial effusion. The disease occurs mostly in 4-10 week-old chickens, 5-week-old chickens are most susceptible, laying hens are rarely affected, and broiler chickens are more common. The causative agent of the disease belongs to group I of avian adenovirus, which has 12 serotypes. The disease was first reported in the United States in 1951. With the rapid development of my country's chicken breeding industry, the disease has shown a local epidemic trend in broiler chicken breeding areas in my country in recent years. , through the search of Wanfang and VIP databases, the preva...

Claims

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Application Information

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IPC IPC(8): A61K39/235A61K39/39A61P31/20C12N7/00
CPCA61K39/12A61K39/39A61K2039/552A61K2039/55522C12N7/00C12N2710/10221C12N2710/10234A61K2300/00
Inventor 魏思远
Owner CHONGQING SANJIE ZHONGXIN BIOLOGICAL ENG CO LTD
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