A microfluidic chip for Raman detection of methamphetamine in hair and its application method

A microfluidic chip, methamphetamine technology, applied in Raman scattering, chemical instruments and methods, laboratory containers, etc., can solve the problems of false positives, short detection time, etc., and achieve reliable detection results and less consumables. , Easy to carry and use

Inactive Publication Date: 2018-03-27
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The traditional methamphetamine detection method is urine analysis, but it has the disadvantages of short detection time limit and prone to false positives. In recent years, the development of hair drug testing has become an effective means of testing addicts. It can dig out the degree of drug use of drug addicts. , so the detection of methamphetamine in the hair has been highly valued by toxicology analysts

Method used

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  • A microfluidic chip for Raman detection of methamphetamine in hair and its application method
  • A microfluidic chip for Raman detection of methamphetamine in hair and its application method
  • A microfluidic chip for Raman detection of methamphetamine in hair and its application method

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Embodiment 1

[0039] see figure 1 , figure 2 , the device structure of the present invention is as follows:

[0040]The main body is in the shape of a cuboid (length X width: 31.5X 15.75mm), which is divided into upper and lower layers, including a base sheet A and a cover sheet B, and the two layers can realize sealed alignment bonding.

[0041] Substrate A includes: labeling solution pool 1 for immunogold sol, washing solution pool 2 for buffer solution, sample reaction and Raman detection area 4, waste solution pool 5 for stop solution , for the detection solution pool 6 of the immune magnetic antibody, for the detection solution pool 7 of the test solution for the hair extraction, wherein:

[0042] The waste liquid pool 5 is located at the rightmost end of the chip, and is connected to the detection area 4 on the left side through the fourth channel 4-1. A magnet is placed on the back of the detection area 4, and the washing liquid pool 2 is located at the leftmost end of the chip. ...

Embodiment 2

[0049] The nanoparticle preparation process of labeled immunogold is;

[0050] Take 49.5ml ultrapure water + 0.5ml HAuCl 3 Stir and disperse evenly, heat and condense to reflux, add 0.5ml Na at one time 3 Cit (trisodium citrate), the color first turns black, purple black, and finally purple red, and then condenses and refluxes for 15 minutes to obtain Au sol with a particle size of about 35nm; take 1ml Au sol + 2.5μl 1mM MBA and incubate for 1.5h, and then centrifugally disperse Add 2 μl 5 μg / ml methamphetamine antigen and incubate for 3.5 hours, disperse by centrifugation, and resuspend in 1 ml of phosphate buffer; add 10 μl of 10% BSA for incubation for 1 hour, and disperse by centrifugation in 1 ml of phosphate buffer.

[0051] The preparation process of the immunomagnetic nanoparticle solution is as follows:

[0052] Take 0.68g FeCl 3 +0.2g ​​Na 3 Cit+20ml EG (ethylene glycol) ultrasonically dissolve, add 1.2g CH 3 COONa was stirred and mixed, and reacted with hydroth...

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Abstract

The invention discloses a microfluidic chip for Raman detection of amphetamin chloride in hair. The microfluidic chip comprises a substrate A and a cover sheet B, and is characterized in that the substrate A comprises a labeling liquid pool (1) for holding an immunogold sol, a washing liquid pool (2) for holding a buffer liquid, a detection zone (4) for a sample reaction and Raman, a waste liquid pool (5) for holding a terminating liquid, a first detection liquid pool (6) for holding an immunomagnetic antibody and a second detection liquid pool (7) for holding a hair extraction liquid to be detected, the waste liquid pool (5) is connected to the detection zone (4) through a fourth channel (4-1), a magnet is placed at the back surface of the detection zone (4), the washing liquid pool (2) is connected to the detection zone (4) through a third channel (2-1), the third channel (2-1) is arranged between the detection zone (4) and the washing liquid pool (2), and the third channel (2-1) is provided with a valve. According to the present invention, the low concentration detection can be achieved, and the obtained result is consistent with the low content of the amphetamin chloride in the actual human hair.

Description

technical field [0001] The field that the present invention relates to is a microfluidic chip and its application field. In particular, it relates to a pretreatment of methamphetamine in hair, a microfluidic device for rapid Raman detection of methamphetamine and a use method thereof. Background technique [0002] Drug abuse addiction and prevalence have become an increasingly serious problem in the world today, such as methamphetamine (MAMP, commonly known as "ice"). Long-term overdose of methamphetamine can lead to mental confusion, hallucinations, and general depression. In severe cases, it can lead to neurobiological changes, damage cognitive functions, and may cause mental disorders. Existing technologies for detecting methamphetamine residues mainly include liquid chromatography, gas-mass spectrometry chromatography, and thin-layer chromatography. Although these methods are accurate in detection, they require expensive large-scale instruments, are time-consuming, and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00G01N21/65G01N33/543
CPCB01L3/5027B01L2200/10G01N21/658G01N33/54326
Inventor 许清良周勇亮陈宏田中群
Owner XIAMEN UNIV
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