Co-culture method for researching interaction between parasitic weeds and host crops

Abstract

The invention discloses a co-culture method for researching interaction between parasitic weeds and host crops. The co-culture method comprises the following steps: firstly, sterilizing surfaces of seeds of the parasitic weeds and the host crops, then performing moist-heat pre-culture on the seeds of the parasitic weeds, and adding germination stimulants for promoting germination; secondly, performing germination and growth on the seeds of the host crops; and finally, uniformly spreading root systems of the host crops on filter paper, covering one side of on which the parasitic weeds are attached to the surface on the root systems of the host crops, and performing co-culture on the parasitic weeds and the host crops. The parasitic weeds directly contact with the root systems of the host crops, so that a process of identifying the root systems of the host crops by the parasitic weeds is greatly shortened, and the parasitic efficiency is improved at the same time; as the experiment is carried out under a soilless condition, the whole culture process is visualized, and every stage of the parasitic weeds parasitizing on the root systems of the host crops is accurately controlled and observed, thereby facilitating research of interaction processes in different stages at exact time points; and the spread of the parasitic weeds during research is surely prevented.

Description

technical field [0001] The invention belongs to the field of research on the interaction between parasitic weeds and host crops, and in particular relates to a co-cultivation method for studying the interaction between parasitic weeds and host crops. Background technique [0002] In nature, seed plants are overwhelmingly autotrophic. However, a small number of seed plants become parasitic due to lack of sufficient chlorophyll or degeneration of roots or leaves, which are called parasitic plants. Parasitic plants obtain all or most of the water and nutrients they need from green plants to survive. There are many types of parasitic plants, mainly divided into Liedangaceae, Dodderaceae, Morusparasiticae, Scrophulariaceae and so on. Most of the parasitic plants are parasitic on wild plants and trees, some of which are valuable medicinal plants; while a small number of parasitic seed plants are parasitic on crops and cause great harm to agricultural production. They are called ...

AI Technical Summary

Problems solved by technology

However, since the root parasitic weeds are parasitic in the underground part of the host crop, it is difficult to observe the whole parasitic process in real time, which is not conducive to the study of the interaction between the root parasitic weeds and the host crop, so it is necessary to invent a method that can be used under soilless conditions. A co-cultivation method that visualizes the entire interaction process

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