Method for degrading sulfonamide compound by using pycnoporus sanguineus

A technology of Microporus hemoglobinus and sulfonamides, which is applied in the field of environmental pollution control, can solve the problems of poor repeatability, cumbersome procedures, high cost, etc., and achieves the effects of accurate repeatability, shortened fermentation time, and improved degradation rate.

Inactive Publication Date: 2017-01-04
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using natural medium for fermentation, the production process is poorly reproducible
In addition, the existing technology uses laccase to degrade sulfonamide compounds, and mostly uses laccase extracted from bacterial fermentation broth or crudely extracted. This series of procedures is cumbersome and expensive

Method used

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  • Method for degrading sulfonamide compound by using pycnoporus sanguineus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The method for degrading sulfonamides by Pycnoporus sanguineus includes the following steps:

[0026] (1) Seed culture: Pycnoporus sanguineus with the preservation number CGMCC 5.815 was cultured in potato dextrose agar solid medium at 28°C for 7 days; then transferred to potato dextrose liquid medium and cultured at 28°C For 48 hours, get the first-grade seeds of Pycnoporus sanguineus;

[0027] (2) Fermentation culture: Take 40 mL of the first-grade seed of P. sanguineus obtained in step (1), centrifuge at 8000 rpm for 10 min, discard the supernatant, and resuspend the bacteria in 460 mL fermentation medium at 28° C., 130 rpm, After 48 hours of fermentation, the laccase activity is 0.85U / mL, see figure 1 ;

[0028] (3) Degradation of sulfa compounds: Mix sulfamethoxazole production wastewater with the fermentation broth obtained in step (2) to make the concentration of sulfamethoxazole 50mg / L, and add 2,2-diazo-bis(3) -Ethyl-benzothiazole-6-sulfonic acid) diammonium salt, m...

Embodiment 2

[0030] The method for degrading sulfonamide compounds with Pycnoporus sanguineus includes the following steps:

[0031] (1) Seed culture: Pycnoporus sanguineus with the deposit number CGMCC 5.815 was cultured in potato dextrose agar solid medium at 25°C for 8 days; then transferred to potato dextrose liquid medium at 25°C for 50 1 hour, get first-grade seeds of Pycnoporus sanguineus;

[0032] (2) Fermentation culture: Take 45 mL of the primary seeds of P. sanguineus obtained in step (1), centrifuge at 6000 rpm for 12 min, discard the supernatant, and resuspend the bacteria in 450 mL of fermentation medium. At 25° C., 100 rpm, Fermentation for 50h; laccase activity is 0.78U / mL;

[0033] (3) Degradation of sulfadiazine compounds: Mix the sulfadiazine production wastewater with the fermentation broth obtained in step (2) to make the concentration of sulfadiazine 20mg / L, and add 2,2-hydrazine-bis(3-ethyl- Benzothiazole-6-sulfonic acid) diammonium salt, the concentration is 0.4mM, and t...

Embodiment 3

[0036] The method for degrading sulfonamides by Pycnoporus sanguineus includes the following steps:

[0037] (1) Seed culture: Pycnoporus sanguineus with the preservation number CGMCC 5.815 was cultured in potato dextrose agar solid medium at 30°C for 6 days; then transferred to potato dextrose liquid medium and cultivated at 30°C 45 1 hour, get first-grade seeds of Pycnoporus sanguineus;

[0038] (2) Fermentation culture: Take 40 mL of the first-grade seed of P. sanguineus obtained in step (1), centrifuge at 10,000 rpm for 8 min, discard the supernatant, and resuspend the bacteria in 500 mL of fermentation medium. At 30° C., 150 rpm, Fermentation for 40h; laccase activity is 0.92U / mL;

[0039] (3) Degradation of sulfa compounds: Mix sulfaisoxazole production wastewater with the fermentation broth obtained in step (2) to make the concentration of sulfisoxazole 35mg / L, and add 2,2-diazo-bis(3) -Ethyl-benzothiazole-6-sulfonic acid) diammonium salt, the concentration is 0.6mM, and the...

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Abstract

The invention discloses a method for degrading a sulfonamide compound by using pycnoporus sanguineus. The method comprises the following steps: (1) culturing pycnoporus sanguineus of which the preservation serial number is CGMCC 5.815 so as to obtain a pycnoporus sanguineus first-grade seed; (2) centrifuging the pycnoporus sanguineus first-grade seed, abandoning the supernate, and resuspending and fermenting a bacterium body by using a fermentation culture medium; (3) mixing sulfonamide compound production discharge wastewater and fermentation broth, adding 2,2-coupled nitrogen-di(3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt, and degrading. By adopting the method disclosed by the invention, not only are culture conditions for rapidly generating laccase by using the pycnoporus sanguineus in the absence of an inducer provided, but also a method for degrading the sulfonamide compound by using the whole bacterium body and an ectoenzyme environment in the fermentation broth is established, and a series of tedious steps such as separation and purification can be avoided. The fermentation time is short, and the degradation rate is high. The degradation process can be accurately repeated, and is very stable.

Description

Technical field [0001] The invention belongs to the field of environmental pollution control, and relates to a method for degrading sulfonamide compounds by Pycnoporus sanguineus. Background technique [0002] Laccases is a protein that binds multiple copper ions and belongs to copper blue oxidase. Laccase can survive in the air, and the only product after the reaction is water, so it is essentially an environmentally friendly enzyme. Because environmental awareness has been gradually valued in recent years, laccase has also become the research object of many scholars in recent years. Laccase is commonly found in fungi and plants. Among them, the laccase produced by white rot fungi plays an important role in the degradation of lignin, and is particularly concerned. Moreover, most of the laccases produced by white rot fungi are extracellular enzymes, which are easy to extract and utilize. The most studied white rot fungi are Trametes and Pleurotus. In recent years, Pycnoporus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F101/38
CPCC02F3/342C02F2101/38C02F2101/40
Inventor 程景胜李馨元英进
Owner TIANJIN UNIV
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