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Compound lactic acid bacteria microecological preparation as well as preparation method and application thereof

A technology of compound lactic acid bacteria and micro-ecological preparations, applied in the field of feed, can solve problems such as overgrowth of bacteria, degradation of strains, and less growth of probiotics

Active Publication Date: 2017-01-04
BEIJING HESWOF BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ordinary Enterococcus faecalis and Enterococcus faecium have not been widely used in feed additives due to their poor acid resistance and high temperature resistance.
[0003] At present, probiotic preparations are widely used in breeding production, but there are still many problems in the preparation technology: (1) The product standards are not uniform, and the production enterprises set their own standards, lacking scientific basis, and the content of probiotics is too high or too low, which affects The effect of use; (2) The source of the production strains is unknown, and the strains used by most enterprises are transferred to each other without separation and breeding, the strains are seriously degraded, and the physiological activity is reduced; (3) The compatibility of the strains lacks scientific basis, and a single strain , double strains, or even multiple strains are randomly matched, which cannot explain the rationality of the compatibility between the strains, and the excellent properties of the strains cannot be achieved. They are complementary through reasonable compatibility, and the synergy is poor; Different characteristics of culture, many production enterprises adopt multi-strain mixed culture instead of fermenter, or even use stacking fermentation, resulting in less growth of probiotics and overgrown bacteria

Method used

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  • Compound lactic acid bacteria microecological preparation as well as preparation method and application thereof
  • Compound lactic acid bacteria microecological preparation as well as preparation method and application thereof
  • Compound lactic acid bacteria microecological preparation as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 Activation culture of Lactobacillus plantarum HEW-A490, Enterococcus faecalis HEW-A131, Enterococcus faecium HEW-A588 and preparation of compound lactic acid bacteria microecological preparation

[0052] 1) Take Lactobacillus plantarum HEW-A490 preserved in a glycerol tube, separate and purify it on the MRS plate, pick a strain with better growth and culture it on the MRS slope for 20 hours, and pick a ring with an inoculation loop in the aseptic operating table Bacteria were inoculated in 100mL MRS liquid medium, cultured with shaking for 20 hours, and then inoculated in the primary seed medium according to 2% inoculation amount, the culture temperature was 30-40°C, the rotation speed was 120-220r / m, and the culture time was 10- 30h, the first grade seed culture solution of Lactobacillus plantarum (viable bacteria number ≥ 2.0 × 10 9 CFU / mL). The fermentation conditions are preferably 37° C., 160 r / m, and cultured for 15 hours.

[0053] The primary seed med...

Embodiment 2

[0064] The preparation of embodiment 2 compound lactic acid bacteria probiotics

[0065] According to the same method as in Example 1, the three strains of Lactobacillus plantarum HEW-A490, Enterococcus faecalis HEW-A131, and Enterococcus faecium HEW-A588 were respectively subjected to seed culture, secondary fermentation, and tertiary fermentation culture, and were centrifuged to obtain For the active sludge of these three strains, the active sludge of Lactobacillus plantarum HEW-A490, Enterococcus faecalis HEW-A131, and Enterococcus faecium HEW-A588 and the protective agent are placed in the mixing tank according to the weight ratio of 0.4:0.8:1.0:1.5 Stir evenly in the medium, adjust the humidity at 30-45% by adding water to obtain microcapsule wet powder, put it into the granulator for granulation, then add the coating liquid to form a coating layer, and then dry to make the moisture Content ≤ 6%, passed through 20 mesh sieve to obtain compound lactic acid bacteria microec...

Embodiment 3

[0066] The preparation of embodiment 3 compound lactic acid bacteria probiotics

[0067] The difference from Example 1 is only that the tertiary fermentation broth obtained in step 4 of Example 1 is centrifuged at 15000r / m centrifuge for 30-40min to obtain the active sludge of complex lactic acid bacteria. Stir the active bacteria slime and protective agent evenly in the mixing tank according to the weight ratio of 2:0.8, and adjust the humidity at 30-45% by adding water to obtain the microcapsule wet powder, put it into the granulator for granulation, and then add The coating solution is used to form a coating layer, and then dried to make the moisture content ≤ 6%, and the compound lactic acid bacteria microecological preparation is obtained through a 20-mesh sieve, and the number of viable bacteria reaches 2.0×10 11 CFU / g. The effective activity of Lactobacillus plantarum HEW-A490 is 1.0×10 11 CFU / g, the effective activity of Enterococcus faecalis HEW-A131 is 5.0×10 10 C...

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Abstract

The invention relates to a compound lactic acid bacteria microecological preparation as well as a preparation method and application thereof. The compound lactic acid bacteria microecological preparation has relatively strong stress resistance, can resist simulated gastric acid, cholate and high-temperature environments, can remain relatively high viable bacteria survival rate, has relatively good stability and can meet the requirements of feed industry, animal husbandry and breeding industry. According to the compound lactic acid bacteria microecological preparation, florae in animal intestines can be regulated, the disease resistance of animals can be improved, the diarrhea rate and the death rate can be decreased, the feed intake of the animals can be further increased, and the feed conversion ratio can be reduced, so that the production performance of animals is improved, and the compound lactic acid bacteria microecological preparation has a good application prospect.

Description

technical field [0001] The invention belongs to the field of feed, and in particular relates to a compound lactic acid bacteria microecological preparation and a preparation method and application thereof. Background technique [0002] The abuse of antibiotics has brought serious threats to animal husbandry and human health. Probiotics can replace antibiotics in aquaculture production. Lactobacillus plantarum (Lactobacillus plantarum) is a normal flora in the intestinal tract of humans and animals. It has important biological functions in inhibiting the growth and reproduction of pathogenic bacteria, promoting the body's digestion and absorption of nutrients, and improving the body's immunity. Wide application prospects. However, most probiotics have poor tolerance to the high temperature, transportation, gastrointestinal fluid and bile of the pelleting process in feed processing, and the types and quantities of live Lactobacillus plantarum entering the intestinal tract ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04A23K10/18C12R1/25C12R1/46
CPCC12N1/04C12N1/20
Inventor 李雪平
Owner BEIJING HESWOF BIOTECH CO LTD
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