Use of sesquiterpene derivatives in aquilaria wood and pharmaceutical compositions thereof

A composition and drug technology, applied in the field of medicine, can solve the problems of metabolic dysfunction, high nephrotoxicity and the like

Active Publication Date: 2017-01-11
BEIJING UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Long-term use of glucocorticoids can lead to metabolic dysfunction, while adaptive immunosuppressants such as cyclosporine and tacrolimus have high nephrotoxicity

Method used

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  • Use of sesquiterpene derivatives in aquilaria wood and pharmaceutical compositions thereof
  • Use of sesquiterpene derivatives in aquilaria wood and pharmaceutical compositions thereof
  • Use of sesquiterpene derivatives in aquilaria wood and pharmaceutical compositions thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1; Compounds 1-4 inhibit the secretion of nitric oxide (NO) in mouse macrophages and microglia in vitro

[0019] (1) Experimental materials

[0020] Cell line: RAW264.7 cells were purchased from the Cell Center of the Institute of Basic Medicine, Chinese Academy of Medical Sciences; BV-2 cells were gifted by researcher Chen Naihong, Chinese Academy of Medical Sciences.

[0021] Reagents: Compounds 1-4 were extracted and isolated from Agarwood by the Modern Research Center of Traditional Chinese Medicine, Beijing University of Traditional Chinese Medicine; bacterial lipopolysaccharide was purchased from Beijing Baierdi Biotechnology Co., Ltd. (Item No.: L2880); Nitric Oxide Determination Kit (Griess Reagent) ) were purchased from Prily Gene Technology Co., Ltd. (Item No.: E1030).

[0022] Instrument: Enspire Multimode Plate Reader was purchased from Platinum Elmer, Germany.

[0023] (2) Experimental method

[0024] RAW264.7 cells (DMEM high-glucose medium) and...

Embodiment 2

[0029] Example 2: Determination of compound 1-4 inhibiting the secretion of inflammatory factors in mouse macrophages in vitro

[0030] (1) Experimental materials

[0031] Cell line: RAW264.7 cells were purchased from the Cell Center of the Institute of Basic Medicine, Chinese Academy of Medical Sciences.

[0032] Reagents: Compounds 1-4 were extracted and isolated from Agarwood by the Modern Research Center of Traditional Chinese Medicine, Beijing University of Traditional Chinese Medicine; bacterial lipopolysaccharide was purchased from Beijing Baierdi Biotechnology Co., Ltd. (Item No.: L2880): Determination of TNF-α and IL-6 ELISA kits were purchased from Wuhan Boster Biotechnology Co., Ltd. (Cat. Nos.: EKO527 and EKO411).

[0033] Instrument: Enspire Multimode Plate Reader was purchased from Platinum Elmer, Germany.

[0034] (2) Experimental method

[0035] RAW264.7 cells in logarithmic growth phase were seeded into 96-well plates at a concentration of 50,000 cells / well...

Embodiment 3

[0040] Example 3: In vitro inhibition of neutrophil activation assay by compounds 1-4

[0041] (1) Experimental materials

[0042] Animals: Balb / c mice were purchased from Beijing Weitong Lihua Laboratory Animal Co., Ltd.

[0043] Reagents: Compounds 1-4 were extracted and isolated from Agarwood by the Modern Research Center of Traditional Chinese Medicine, Beijing University of Traditional Chinese Medicine; PE-labeled rat anti-mouse CD11b antibody was purchased from BD Biosciences in the United States (Cat. No. 557397).

[0044] Instrument: FACSCanto TM II flow cytometer was purchased from BD Biosciences, USA.

[0045] (2) Experimental method

[0046]One Balb / c mouse was killed by cervical dislocation, soaked in 75% alcohol for 5 minutes, then aseptically removed the femur on both sides of the mouse in an ultra-clean workbench, cut the femoral knee joint end, and sucked IMDM medium (containing 2 % fetal bovine serum) to flush the bone marrow cells from the femur into a c...

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PUM

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Abstract

The invention discloses application of sesquiterpene derivatives extracted and separated from aquilaria wood and pharmaceutical compositions thereof in prevention or treatment of inflammatory diseases, autoimmune diseases and organ transplant rejection, especially in prevention or treatment of pneumonia, bronchitis, hepatitis, enteritis, systemic informatory response, rheumatoid arthritis, systemic lupus erythematosus, renal transplantation rejection, liver transplantation rejection and hematopoietic stem cell transplantation rejection.

Description

technical field [0001] The invention relates to the application of four sesquiterpene derivatives in preventing or treating inflammation-related diseases, autoimmune diseases and organ transplant rejection, and belongs to the technical field of medicine. Background technique [0002] The human immune system, including innate immunity and adaptive immunity, plays an important role in defending against damage caused by various internal and external factors. But overactive immune function can also lead to severe tissue damage. Diseases related to excessive activation of immune function mainly include inflammation-related diseases, autoimmune diseases and organ transplant rejection. Excessive activation of innate immunity mainly leads to inflammation-related diseases or aggravates the body damage of inflammation-related diseases. Excessive activation of adaptive immunity leads to autoimmune diseases and organ transplant rejection. [0003] At present, the drugs for the preven...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/11A61P29/00A61P37/02A61P37/06A61P11/00A61P1/16A61P13/12A61P1/04A61P25/00A61P17/00A61P19/02A61P7/06
Inventor 屠鹏飞李军朱枝祥霍会霞赵云芳宋月林
Owner BEIJING UNIV OF CHINESE MEDICINE
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