Xenogeneic acellular dermis and preparation method thereof

An acellular and dermis technology, applied in the field of acellular xenogeneic dermis and its preparation, can solve problems such as collagen damage and unfavorable wound recovery, and achieve the effects of reducing negative effects, facilitating recovery as soon as possible, and avoiding acute immune response of the human body.

Inactive Publication Date: 2017-01-11
江西瑞诺健医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although repeated freezing and thawing techniques can effectively destroy cells, each freezi...

Method used

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  • Xenogeneic acellular dermis and preparation method thereof
  • Xenogeneic acellular dermis and preparation method thereof
  • Xenogeneic acellular dermis and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0045] see figure 1 and figure 2 , a method for preparing decellularized heterogeneous dermis, comprising the following steps in sequence,

[0046] Step A: Prepare pig dermis slices. The specific operation is to clean the back skin of the mature large white pig (Yorkshire pig breed), and after removing the hair with the depilator, use the skin drum to cut a piece of skin with a thickness of 0.25 mm and discard it (function: remove the epidermis, hair and hair follicles). Then use the skin drum to take a 0.50-0.75 mm thick dermis for later use. Those skilled in the art should know that the raw materials are not limited by the breed and age of pigs, and the back skin of mature large white pigs is only a preferred method. The preferred reason is that it is convenient to obtain materials and there are many available raw materials.

[0047] Step B: Disinfect the pig dermis slices. The specific operation is to put the pig dermis slices into a sterile bottle, add 0.5% peracetic ...

Embodiment 2

[0055] see figure 1 , figure 2 and image 3 , on the basis of embodiment 1, this embodiment also has the following steps in sequence after step G,

[0056] Step H: Add the ribonuclease solution, and carry out thorough mixing and reaction, the ribonuclease in the ribonuclease solution is synthesized by artificial recombination or directly extracted from animal tissue, and the ribonuclease solution is 4-hydroxyethyl Piperazineethanesulfonic acid buffer, each liter of 4-hydroxyethylpiperazineethanesulfonic acid buffer contains 20 mmol 4-hydroxyethylpiperazineethanesulfonic acid, 2.5 mmol magnesium chloride, 0.5 mmol calcium chloride and 0.5 Gram ribonuclease, its pH value is 8, and the ratio of ribonuclease solution volume and pig dermis sheet volume is 20:1.

[0057] Step I: Aspirate the ribonuclease solution, then add phosphate buffered saline to fully wash the pig dermis slices. The specific operation is to suck out the ribonuclease solution, wash with phosphate-buffered ...

Embodiment 3

[0060] see figure 1 , figure 2 , image 3 and Figure 4 , present embodiment is on the basis of embodiment 2, also has following steps successively after step 1,

[0061] Step J: adding alpha-galactosidase solution for thorough mixing and reaction, said alpha-galactosidase solution is prepared by adding alpha-galactosidase to citrate buffer solution. The specific operation is to shake on a shaker for 10 hours.

[0062] Step K: Aspirate the alpha-galactosidase solution, then add phosphate-buffered saline to thoroughly wash the porcine dermal slices. The specific operation is aspirating the alpha-galactosidase solution, washing with phosphate-buffered saline three times, and shaking on a shaker for 30 minutes each time.

[0063] In this embodiment, steps J~K are alpha-galactose removal methods. The alpha-galactose referred to in this example is Galactose-alpha-1,3-galactose, and the alpha-galactosidase referred to is galactose-alpha-1,3-galactosidase. Galactose-alpha-1,3...

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Abstract

The invention discloses xenogeneic acellular dermis and a preparation method thereof. The preparation method comprises steps of acquiring and preserving a pig dermal slice and conducting acellular treatment, wherein the pig dermal slice is preserved with the addition of a cryoprotective agent, and for the acellular treatment, a mild detergent is adopted to break cells. According to the xenogeneic acellular dermis and the preparation method thereof provided by the invention, freeze-thaw cycles are avoided, and by adding the cryoprotective agent in a freezing process, damage to the bio-activity of collagen is relieved and the rapid recovery of a wound surface is promoted; residual ribonucleic acid is effectively removed by virtue of a ribonuclease solution, so that an adverse reaction caused by the residual ribonucleic acid is avoided; [alpha]-galactose is effectively removed by virtue of an [alpha]-galactosidase solution, so that an acute immune response in human body caused by the [alpha]-galactose is relieved and even avoided; by conducting electron beam end sterilization in an anti-radiation protective agent or by conducting [gamma] radiation under a dry ice condition, damage of the radiation to the pig dermal slice is reduced while conducting the sterilization; and the xenogeneic acellular dermis, which is packaged in an aluminum foil inside and outside dual-layer bag, can be conveniently opened and used by a doctor.

Description

technical field [0001] The invention relates to the field of medical materials for clinical application in medical burns and plastic surgery, in particular to a decellularized heterogeneous dermis and a preparation method thereof. Background technique [0002] As we all know, in the clinical practice of medical burns and plastic surgery, patients with severe trauma and extensive burns have huge wounds with skin loss. Skin transplantation is required to eliminate wounds during treatment. The skin source used can be homogeneous dermis or heterogeneous dermis. Due to insufficient sources of homogeneous dermis, at present, heterogeneous dermis is mainly used for skin transplantation. [0003] There is a key technical point in the use of heterogeneous dermis for skin transplantation: to minimize the damage to the three-dimensional structure of the dermal matrix during decellularization, and at the same time ensure that the collagen has sufficient biological activity to provide th...

Claims

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Application Information

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IPC IPC(8): A61L27/60A61L27/36
Inventor 雷良伟
Owner 江西瑞诺健医学科技有限公司
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