Method for preparing coarse nosiheptide product by utilizing nosiheptide fermentation liquor
A technology of Nosiheptide and fermentation liquid, which is applied in the field of preparing Nosiheptide crude product by using Nosiheptide fermentation liquid, which can solve the problems of low total yield of extraction process and high production cost, so as to improve extraction yield, reduce production cost, The effect of simple process
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Embodiment 1
[0022] Nosiheptide Fermentation Broth 100m 3 , after testing, its potency is 1630ug / ml.
[0023] 1) Fermentation broth pretreatment
[0024] The temperature of the Nosiheptide fermentation broth was controlled at 30°C, the stirring speed was controlled at 90r / min, and 10kg of urea and 40kg of triton X-100 were added to the fermentation broth. Stirring was continued for 150 min, then allowed to stand for 40 min. The fermentation broth was filtered to obtain 37 tons of wet solid A.
[0025] 2) Washing
[0026] Add 300 tons of purified water to wet solid A, control the stirring speed at 40r / min, stir for 20 minutes, obtain wet solid B after filtration, add 300 tons of purified water again, stir for 20 minutes, and filter to obtain 36.7 tons of wet solid C.
[0027] 3) Extraction
[0028] Add organic solvent dioxane 147m in wet solid C 3 , the stirring speed is controlled at 20r / min, stirred for 180min, and the mixed solution D152.3m is obtained after filtration 3 . Add 22...
Embodiment 2
[0033] Nosiheptide Fermentation Broth 100m 3 , after testing, its titer is 1607ug / ml.
[0034] 1) Fermentation broth pretreatment
[0035] The temperature of the Nosiheptide fermentation broth was controlled at 31°C, the stirring speed was controlled at 95r / min, and 15kg of urea and 45kg of triton X-100 were added to the fermentation broth. Stirring was continued for 160 min, then allowed to stand for 45 min. The fermentation broth was filtered to obtain 36.7 tons of wet solid A.
[0036] 2) Washing
[0037] Add 312 tons of purified water to wet solid A, control the stirring speed at 45r / min, stir for 25 minutes, obtain wet solid B after filtration, add 312 tons of purified water again, stir for 25 minutes, and filter to obtain 36.5 tons of wet solid C.
[0038] 3) Extraction
[0039] Add organic solvent pyridine 165m to wet solid C 3 , the stirring speed is controlled at 25r / min, stirred for 185min, and the mixed solution D172m is obtained after filtration 3 . Add 237...
Embodiment 3
[0044] Nosiheptide Fermentation Broth 100m 3 , after testing, its potency is 1547ug / ml.
[0045] 1) Fermentation broth pretreatment
[0046] The temperature of the Nosiheptide fermentation broth was controlled at 33°C, the stirring speed was controlled at 100r / min, and 20kg of urea and 50kg of triton X-100 were added to the fermentation broth. Stirring was continued for 170 min, then allowed to stand for 50 min. The fermentation broth was filtered to obtain 35.8 tons of wet solid A.
[0047] 2) Washing
[0048] Add 322 tons of purified water to wet solid A, control the stirring speed at 50r / min, stir for 30 minutes, obtain wet solid B after filtration, add 322 tons of purified water again, stir for 30 minutes, and filter to obtain 35.7 tons of wet solid C.
[0049] 3) Extraction
[0050] Add organic solvent dioxane 179m in wet solid C 3 , the stirring speed is controlled at 30r / min, stirred for 190min, and the mixed solution D186.4m is obtained after filtration 3 . Add...
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