Ginseng growth-promoting bacteria ji39-2 and its application
A technology of bacteria and ginseng, applied in the field of microorganisms, can solve the problems of sustainable soil production capacity, decline in fertilizer utilization rate, etc.
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Embodiment 1
[0018] Screening and Identification of Example 1 Bacterial Strain TY15-2
[0019] The ginseng endophytic bacteria JI39-2 was screened from healthy ginseng plants in Tonghua, Jilin Province by plate dilution method.
[0020] Streak the JI39-2 strain on the NA medium and culture it at 28°C for 24 hours. Observe that the strain JI39-2 on the NA medium shows a light yellow opaque circular small colony with neat edges, such as figure 1 shown.
[0021] Take the JI39-2 bacterial solution cultured in LB liquid medium for 24 hours, centrifuge and wash it repeatedly with sterile water, suspend in an appropriate amount of sterile water for negative staining to prepare samples, and observe under the transmission electron microscope that the bacteria are rod-shaped, with perinatal flagella, such as figure 2 shown.
[0022] The physiological and biochemical characteristics of the JI39-2 strain were determined by referring to some culture media (Table 1) and methods recommended in the "...
Embodiment 2
[0026] Example 2 Determination of growth-promoting properties of bacterial strain JI39-2
[0027] 1. Determination of Phosphorus Solubilizing Ability
[0028] Inoculate the strain JI39-2 on the NA medium, inoculate the plate after 12 hours of cultivation, inoculate in the PVK solid medium and cultivate for 3 days to observe the size of the phosphorus-dissolving circle. The results show that the strain JI39-2 has a clearly visible phosphorus-dissolving circle, as Figure 4 shown; then the quantitative determination of phosphorus solubilization was carried out by the molybdenum antimony scandium colorimetric method. The method was to inoculate the strain JI39-2 in 50mL LB liquid medium and cultivate it at 160 r / min for 12h. Take 1 mL and inoculate it in a Erlenmeyer flask containing 50 mL of PVK liquid medium. After 3 days, take the culture solution and centrifuge at 8000 r / min for 5 minutes, then take the supernatant. The soluble phosphorus content can reach 164.2 µg as measure...
Embodiment 3
[0036] Embodiment 3: Effect of bacterial strain JI39-2 on promoting the growth of ginseng
[0037] 1. Seed germination promotion test
[0038] After the strain JI39-2 was inoculated in LB culture medium, the cells were collected by centrifugation at 12000 rpm for 5 min. Wash twice with sterile water, resuspend in sterile water, and dilute to a concentration of 10 9 cfu / mL of bacterial suspension. Soak the post-ripened ginseng seeds in the bacterial suspension for 3 hours, take them out and dry them, and place them in a petri dish filled with sterile moist sand. There are 15 seeds in each dish, which is 1 repetition, a total of 6 repetitions. Place them at 18-20°C for 12-15 days to accelerate germination, and use sterile water-treated seeds as a control. The results show (Table 2, Figure 5 , Figure 6 ), the average shoot length of ginseng seeds treated with the JI39-2 strain was 20.51 mm, which was greater than that of the water control treatment (12.46 mm), and the sho...
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