Ceriporia lacerata strain and culture method and application thereof

A technology for tearing C. cereus and culture medium, which is applied in the field of C. tearing and its cultivation, can solve the problems of high preparation cost of bio-based materials and strict requirements for cultivation conditions, and achieves the reduction of preparation cost and resistance to miscellaneous bacteria. powerful effect

Active Publication Date: 2017-01-11
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although there are currently some reports on the use of microorganisms to prepare bio-based materials, the liquid culture process and/or solid culture

Method used

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  • Ceriporia lacerata strain and culture method and application thereof
  • Ceriporia lacerata strain and culture method and application thereof
  • Ceriporia lacerata strain and culture method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation Embodiment 1

[0046] The strains of the cereus sp. (preservation number: CGMCC No. 10485, hereinafter referred to as YY bacteria) were transferred into the slant of the Kerner flask, the culture medium was PDA medium, and cultured at 25° C. for 7 days to obtain the strains of the slant.

[0047] The slant strains were inserted into the primary seed liquid culture medium. The mass percentage formula of the culture medium was: 2% soluble starch, 0.6% dry corn steep liquor powder, 0.1% potassium dihydrogen phosphate, natural pH, and sterilized at 121°C for 20 minutes. Cultivation conditions: liquid volume is 150mL / 500mL baffle Erlenmeyer flask, inoculum size is about 3cm 2 The bacterial lawn is cultivated at 25° C. on a shaker at 150 rpm for 3-4 days to obtain a first-grade seed liquid.

[0048] The first-level seed liquid is connected to the second-level seed liquid medium, and the mass percentage formula of the medium is: cornstarch 6%, corn steep liquor dry powder 0.8%, potassium dihydrogen...

Embodiment 1

[0050] This example is used to illustrate the culture characteristics of the strains of the present invention.

[0051] (1) Culture (25° C., 150 rpm, 7 days) YY bacteria with sterilized and non-sterilized secondary seed liquid medium (composition as shown in Preparation Example 1) respectively, and YY bacteria can grow, as figure 2 as shown ( figure 2 A represents the form after culturing with sterilized liquid medium, figure 2 B represents the morphology after culturing with non-sterile liquid medium). There is no obvious difference between the shape of the mycelium mass cultured in non-sterilized liquid medium and the sterilized medium, there are milky white bacterial circles above the liquid surface, no signs of bacterial contamination, and the smell of the fermentation broth and sterilization conditions The cultured YY bacteria were the same. It shows that YY bacteria has the characteristics of fast growth and strong resistance to miscellaneous bacteria.

[0052] (2...

Embodiment 2

[0056] This example is used to illustrate the application of the bacterial strain of the present invention in the preparation of degradable materials.

[0057]The seed liquid obtained in Preparation Example 1 and the non-sterilized medium (the mass percent of the medium composition is: soybean stem (with a particle diameter within the scope of 0.1-15 mm, and a particle diameter of soybean stem and particle diameter below 2 mm) The weight ratio of soybean stalks above 2mm is 1:1)99%, gypsum 1%) mixed, the inoculum size is 4g / kg culture medium, open culture is carried out at 25°C, and the relative humidity of the culture environment is 85%: Pre-cultivate for 3 days to make the mycelium fully grow in the culture medium. After the pre-cultivation, the cultured materials are scattered and placed in the mold for 5 days of in-mold culture. After the mycelium is covered with the culture medium, the mold is demoulded. Cultured outside the mold for 2 days. Dry at 55°C for 10 hours to o...

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Abstract

The invention relates to the field of industrial microorganisms and discloses a ceriporia lacerata strain. The ceriporia lacerata strain has a collection number of CGMCC No.10485. The invention also discloses a method for culturing the ceriporia lacerata strain. The method comprises a step of inoculating the ceriporia lacerata strain into a culture medium for culturing. In addition, the invention also discloses application of the ceriporia lacerata strain in preparation of degradable materials. The mycelium of the ceriporia lacerata strain disclosed by the invention is capable of winding and fixing scraps of plant medium materials. By virtue of the ceriporia lacerata strain disclosed by the invention, plant materials can be prepared into the degradable materials under the condition that sterilization or bacteriostatic treatment is not performed, an aseptic environment is not needed in the preparation process, and the capacity of resisting infectious microbes is high; and moreover, because the ceriporia lacerata strain disclosed by the invention has high capacity of resisting infectious microbes, the various culture processes (liquid culture or solid culture) can be performed under non-sterile conditions; and therefore, the preparation cost of the degradable materials can be greatly reduced.

Description

technical field [0001] The invention belongs to the field of industrial microorganisms, and relates to Ceriporia lacerata and its cultivation method and application. Background technique [0002] Foam plastic is a common thermal insulation, thermal insulation and cushioning material, and has a wide range of applications in many fields such as construction, packaging, filling, and energy absorption. However, foam plastics face two major problems: one is that the raw material comes from petroleum, such as every 1m produced 3 Polystyrene foam requires 65L of petroleum, and petroleum is limited. my country's foreign dependence on petroleum has exceeded 60%, and it is imperative to seek substitutes for petroleum-based foam; second, it is difficult to be decomposed in the natural environment , Seriously endanger the ecological environment and living environment. Plastic waste drifting in rivers and lakes and mixed in pastures has threatened fish and livestock. For example, a lar...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P19/14C12R1/645
Inventor 姜文侠杨萍邵国兵刘琦
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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