Unlock instant, AI-driven research and patent intelligence for your innovation.

Space microbacterium LCT-H2

A technology of LCT-H2 and microbacteria, which is applied in the direction of bacteria, microorganisms, microorganisms, etc., and can solve the problem that genetic information is difficult to obtain

Inactive Publication Date: 2017-01-11
GENERAL HOSPITAL OF PLA
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Early people's understanding of many environmental microorganisms only came from the study of 16S rRNA, and some important genetic information is difficult to obtain

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Space microbacterium LCT-H2
  • Space microbacterium LCT-H2
  • Space microbacterium LCT-H2

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Implementation example one: strain LCT-H2 culture.

[0017] The strain LCT-H2 was isolated from the condensed water in the return cabin of the "Shenzhou 9" spacecraft. The strains were cultured statically or shaken in a 37°C incubator or shaker. The liquid medium was brain heart infusion medium (BHI, Oxiod) with a formula of 37 g / L BHI; the solid medium was MH plates.

Embodiment 2

[0018] Implementation example two: strain LCT-H2 strain identification and phylogenetic tree construction.

[0019] LCT-H2 was inoculated into BHI liquid medium, cultivated overnight at 37 °C, 180 rpm, centrifuged at 6000 rpm for 5 min to collect the bacteria, and discarded the supernatant. The genomic DNA of the collected bacteria was extracted using the Genomic DNA Extraction Kit of Qiagen (Code No. 51304, see the product manual for detailed steps). Using the genomic DNA of Microbacterium sp. LCT-H2 as a template, the 16s rRNA fragment was amplified and sequenced using bacterial 16s rRNA universal sequencing primers 27F (5'- AGAGTTTGATCCTGGCTCAG-3') and 1492R (5'-TACGGCTACCTTGTTACGACTT-3'), Get its base sequence. Multiple sequence alignments were performed with CLUSTALW (http: / / www.ebi.ac.uk / Tools / msa / clustalw2 / ) and NJ phylogenetic trees were constructed with MEGA 5.0 (3).

Embodiment 3

[0020] Implementation example three: Gram staining of bacterial strain LCT-H2.

[0021] LCT-H2 cells were stained with Gram staining kit (Code No. G1060, Solarbio). Cultivate the bacteria overnight in BHI liquid medium, drop the bacteria solution on the center of the glass slide, and warm it slightly on the alcohol lamp to make it dry quickly. Crystal violet and sand yellow staining solution can be used to stain and decolorize the dried bacteria at one time. For detailed steps, please refer to the product manual. After the staining was completed and dried, the staining was observed under an optical microscope.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a down microbacterium strain LCT-H2 (Microbacterium sp.LCT-H2) separated in a Shenzhou IX airship re-entry capsule. The strain is a short gram-positive bacterium, and multiple carbon sources such as glucose, cellobiose and the like can be utilized. The strain is subjected to 16S rRNA sequencing and is identified as a microbacterium member. Whole genome sequencing analysis is performed, the genome size is 3.36Mbp, the strain comprises 3235 coding sequences and 53 RNA genes, and COG database alignment totally comments 21 groups of COG functional classifications. A certain help is provided for researching microbial species distribution in an airtight aircraft and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology; more specifically, the invention relates to microbacterium LCT-H2, its biological characteristics, and whole genome sequencing. Background technique [0002] With the development of aerospace technology, human exploration activities in outer space are becoming more and more frequent. Microorganisms are found almost everywhere on the ground, most of which are found on the human skin, oral cavity, nasopharynx and gastrointestinal tract. Although the manned spacecraft and its internal items have undergone strict vacuum disinfection, microorganisms such as bacteria and fungi are still easy to breed in the airtight environment of manned spacecraft, and these microorganisms can form biofilms on the surfaces of metals and polymer composite materials in airtight cabins , their growth, reproduction and metabolism will corrode materials, which seriously threatens the long-term on-orbit operation safety of the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/205C12R2001/01
Inventor 刘长庭张学林周宏方向群姜学革徐绸刘岩潘磊黄兵李佳余昳
Owner GENERAL HOSPITAL OF PLA
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com