Establishing method of scrophularia ningpoensis tissue culture system

Abstract

The invention discloses an establishing method of a scrophularia ningpoensis tissue culture system. Scruphularia ningpoensis has effects of clearing heat and purging pathogenic fire, nourshing yin to promote salivation and treating heat pain and yin impairement, damp toxin and spotting, body fluid impairment constipation, eye-throat soreness, diphtheria and pain, swelling and sore-toxin. Therefore, planting scrophularia ningpoensis has good development prospect, and scrophularia ningpoensis is a good economic plant. Plant tissue culture technology is utilized to improve breeding speed and scale of scrophularia ningpoensis, so that industrialized production of scrophularia ningpoensis seedlings is realized. Stems with buds are used as explants, scrophularia ningpoensis tissue culture regenerated plants are successfully obtained through steps of inducing, breeding, rooting and acclimatization and transplanting, and a systematic scrophularia ningpoensis tissue culture plant regeneration system is established. The establishing method has the advantages of simple technique, easiness in operation and quick material regeneration.

Description

technical field [0001] The invention relates to the field of Scrophulariae planting, especially a method for establishing a Scrophulariae tissue culture system. Background technique [0002] Scrophulariaceae is a family plant, the dry root of Scrophularia ningpoensis Hemsl is mainly produced in Zhejiang, Sichuan, Shaanxi, Guizhou, Hubei, Hunan and other provinces and regions. Excavate when the stems and leaves wither in winter, remove the stems, leaves, root buds, and sediment, and cut off the reed heads and fibrous roots when they are sun-dried or dried until half-dried, and then dried or dried after stacking for 4-6 days. Properties: the root is cylindrical, slightly thick in the middle or thick at the top and thin at the bottom, some are slightly curved, 6-20cm long, 1-3cm in diameter, gray-yellow or gray-brown on the surface, with irregular longitudinal grooves, transverse lenticels and Sparse transverse cracks and fibrous root marks. The fruit is solid, not easy to br...

AI Technical Summary

Problems solved by technology

When using seeds for propagation, the seeds have the problems of difficult germination and low germination rate, which greatly reduces the survival rate and seedling rate, and the cost is high and the income is low; when using cuttings and layers for propagation, the cuttings are not easy to take root Or it is relatively slow and not easy to survive, which also causes the problem of low seedling rate of Scrophulariaceae