Method for inhibiting browning of perennial Mongolian oak explants

A technology of explants and Quercus mongolica, which is applied in botany equipment and methods, horticultural methods, plant regeneration, etc., can solve the problems that seedlings cannot maintain good properties, difficult to root, and the survival rate of perennial Quercus mongolica cuttings is low.

Pending Publication Date: 2017-02-01
辽宁省蚕业科学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Quercus mongolica has many excellent natural hybrid varieties in nature. Traditional seed seedlings cannot maintain the original excellent traits. Although cutting seedlings can mai...

Method used

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  • Method for inhibiting browning of perennial Mongolian oak explants
  • Method for inhibiting browning of perennial Mongolian oak explants
  • Method for inhibiting browning of perennial Mongolian oak explants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] 1. Effects of different concentrations of antioxidant solutions on browning of perennial Quercus mongolica explants

[0046] The sterilized explants were cut and soaked in different concentrations of antioxidant solutions for 10 minutes, then inoculated on WPM (blank) medium, and the anti-browning rate was investigated after 7 days. The results are shown in Table 1.

[0047]

[0048] Table 1 Effects of different concentrations of antioxidant solutions on browning of perennial Quercus mongolica explants

[0049]

[0050] It can be seen from Table 1 that different concentrations of antioxidant solutions have different anti-browning effects on perennial Quercus mongolica explants, and the mixed solution of 0.1% citric acid and 0.2% vitamin C has the best effect on inhibiting the browning of explants. The anti-browning rate reaches 40.68%, and the anti-browning effect of 0.2% citric acid and 0.2% vitamin C mixture can also reach 38.24%. The anti-browning rate of expl...

Embodiment 2

[0052] 2. Effect of soaking time of explants in antioxidant solution on browning of perennial Quercus mongolica explants

[0053] The sterilized explants were soaked in 0.1% citric acid and 0.2% vitamin C mixture for 5min, 10min, 15min, 20min, 30min respectively, and then inoculated on WPM (blank) medium, and the browning was investigated after 10 days. The results are shown in Table 2

[0054] Table 2 Effects of different soaking times on browning of perennial Quercus mongolica explants

[0055]

[0056]

[0057] It can be seen from Table 2 that when the soaking time is 15 minutes, the anti-browning rate of the explants is the highest, and the anti-browning rate of the explants decreases after 20 minutes. It shows that soaking in antioxidant solution for too long will cause damage to explants.

Embodiment 3

[0059] 3. Effect of basic medium on browning of perennial Quercus mongolica explants

[0060] Soak the sterilized explants with antioxidant solution (0.1% citric acid and 0.2% vitamin C mixture) for 15 minutes and then inoculate them on different basic medium, the additional components of which are 6-benzylaminopurine 1.0mg / L, 6-furfurylaminopurine 2.0mg / L, α-naphthaleneacetic acid 0.5mg / L. Investigate the brown mortality rate after 10 days, and investigate the induction rate after 14 days.

[0061]

[0062] Table 3 Effects of different basal media on browning of perennial Quercus mongolica explants

[0063] experimental code basic medium Anti-browning rate (%) Induction rate (%) growth state 16 WPM 43.79 35.24 robust, bright green leaves 17 1 / 2WPM 46.87 39.47 robust, bright green leaves 18 MS 30.12 18.17 robust, bright green leaves 19 HB 32.47 10.23 Robust, yellow-green leaves

[0064] It can be seen from Table...

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Abstract

The invention relates to a propagation technology of forest seeds and seedlings, in particular to a method for inhibiting browning of perennial Mongolian oak explants. The stem segments with axillary buds of aquatic Mongolian oak branch germinated beds are taken as the explants, disinfection treatment is conducted on the explants, then cutting and soaking are performed in an anti-oxidation solution, then the explants are inoculated in a culture medium added with mashed banana and an anti-browning agent, culture is performed in a dark environment at a preliminary inoculation stage, the explants are transferred to a conventional culture environment after 4-8 hours and then are transferred to a novel culture medium after 14 days for conventional culture. By the adoption of the method for inhibiting browning of the perennial Mongolian oak explants, the browning rate of the perennial Mongolian oak explants can be remarkably reduced, the induction rate of the perennial Mongolian oak explants can be improved, the method is simple in operation and good in browning inhibiting effect, and a technical support is provided for tissue culture and vegetative propagation of perennial Mongolian oaks.

Description

technical field [0001] The invention relates to forest tree seedling propagation technology, in particular to a method for inhibiting the browning of perennial Quercus mongolica explants Background technique [0002] Mongolian oak (Quercus mongolica Fischer ex Ledebour) belongs to Fagaceae and belongs to the genus Quercus. Deciduous tree, light-loving, cold-resistant, resistant to -50°C, and cool climate; drought-resistant, barren-resistant, neutral to acidic soil, and well-developed root system. It is one of the main sericulture species in Liaoning, Jilin, Heilongjiang, Inner Mongolia and other provinces and regions one. [0003] Mongolian oak is hard, heavy in proportion, beautiful in texture, resistant to corrosion and moisture, and can be used for construction, shipbuilding, sleepers, and plywood. Compressed wood can be used to make mechanical parts. The bark can be used medicinally and can restrain diarrhea. The shell and bark contain tannins (16.73% in the shell an...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 于艳郎庆龙夏兴宏孙娟费腾高伟王连珍李立峰曲芳左奎华
Owner 辽宁省蚕业科学研究所
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