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A kind of nitrifying bacteria microcapsule bacterial agent for purifying water and preparation method thereof

A technology of nitrifying bacteria and microcapsules, applied in the field of ecological environment protection, can solve the problems of low activity of nitrifying bacteria, difficult to disperse, poor purification effect, etc., and achieve the effects of improving bacterial activity, prolonging storage time and simple operation process.

Active Publication Date: 2019-10-29
中科众沃生态科技(武汉)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the problems of low activity of nitrifying bacteria after being immobilized by existing nitrifying bacteria immobilization technology, difficulty in dispersing after being put into water, and poor purification effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The present embodiment provides a kind of preparation method of the nitrifying bacteria microcapsule inoculum agent that is used for purifying water, comprises the steps:

[0024] 1) Add 0.5 g of nitrifying bacteria to 1000 g of fermented liquid for fermentation.

[0025] 2) Step 1) After the fermentation is completed, the solid matter in the fermentation broth is filtered out, and the fermentation broth is concentrated at a low temperature by vacuum distillation at a temperature of 40° C., and the concentrated concentration is 10%.

[0026] 3) In the concentrated fermentation broth, add Span 80 with a weight ratio of 1‰ to the fermentation broth, lecithin with a weight ratio of 0.6‰ to the fermentation broth, and polyvinylpyrrolidone with a weight ratio of 0.5‰ to the fermentation broth K30, starch with a weight ratio of 3‰ to the fermentation broth, stirred and dissolved evenly, emulsified with a high-speed shear emulsifier, and finally spray-dried to make nitrifying ...

Embodiment 2

[0028] The present embodiment provides a kind of preparation method of the nitrifying bacteria microcapsule inoculum agent that is used for purifying water, comprises the steps:

[0029] 1) Add 0.6 g of nitrifying bacteria to 1000 g of fermented liquid for fermentation.

[0030] 2) Step 1) After the fermentation is completed, the solid matter in the fermentation broth is filtered off, and the fermentation broth is concentrated at a low temperature by vacuum distillation at a temperature of 55° C., and the concentrated concentration is 20%.

[0031] 3) In the concentrated fermentation broth, add Span 80 with a weight ratio of 1.5‰ to the fermentation broth, lecithin with a weight ratio of 1‰ to the fermentation broth, and polyvinylpyrrolidone with a weight ratio of 0.7‰ to the fermentation broth K30, starch with a weight ratio of 5‰ to the fermentation liquid, stirred and dissolved evenly, emulsified with a high-speed shearing emulsifier, and finally spray-dried to make nitrify...

Embodiment 3

[0033] The present embodiment provides a kind of preparation method of the nitrifying bacteria microcapsule inoculum agent that is used for purifying water, comprises the steps:

[0034] 1) Add 0.55 g of nitrifying bacteria to 1000 g of fermented liquid for fermentation.

[0035] 2) Step 1) After the fermentation is completed, the solid matter in the fermentation broth is filtered out, and the fermentation broth is concentrated at a low temperature by vacuum distillation at a temperature of 45° C., and the concentrated concentration is 15%.

[0036] 3) In the concentrated fermentation broth, add Span 80 with a weight ratio of 1.2‰ to the fermentation broth, lecithin with a weight ratio of 0.8‰ to the fermentation broth, and polyvinylpyrrolidone with a weight ratio of 0.6‰ to the fermentation broth K30, starch with a weight ratio of 3.5‰ to the fermentation broth, stirred and dissolved evenly, emulsified with a high-speed shear emulsifier, and finally spray-dried to make nitrif...

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Abstract

The invention provides nitrifying bacteria microcapsule inocula for water purification. The nitrifying bacteria microcapsule inocula for the water purification comprise microcapsules and nitrifying bacteria fermentation liquid, wherein the microcapsules comprise the following components in percentage by weight of the fermentation liquid: 0.1 to 0.15 % of span 80, 0.06 to 0.1 % of lecithin, 0.05 to 0.07 % of polyvinylpyrrolidone K30 and 0.3 to 0.5 % of starch. According to the nitrifying bacteria microcapsule inocula, polymer materials are used to form a layer of microcapsule protective film on the surface of each nitrifying bacterium, so that the nitrifying bacteria can be separated from the external environment, and then the storage time of the nitrifying bacteria is prolonged, and the activity of the nitrifying bacteria is improved; furthermore, the nitrifying bacteria microcapsule inocula are easily dispersed in water and can rapidly disintegrate and uniformly release the nitrifying bacteria while meeting water, and the purification efficiency is higher.

Description

technical field [0001] The invention belongs to the technical field of ecology and environmental protection, and specifically relates to a nitrifying bacteria microcapsule agent for water purification and a preparation method thereof, which are used for purifying water bodies. Background technique [0002] Nitrifying bacteria is a kind of aerobic bacteria, including nitrous acid bacteria and nitric acid bacteria, living in aerobic water or sand layer, and plays an important role in the nitrogen cycle water purification process. Nitrifying bacteria are autotrophic bacteria, prokaryotes, including two completely different metabolic groups: nitrosomonas and nitrobacter, which include bacilli, cocci and spirochetes with different shapes; nitrous Bacteria include bacteria in the genera Nitrosomonas, Nitrosococcus, Nitrospirilla and Nitrosolobus; Nitrobacter include bacteria in the genera Nitrobacter, Nitrococcus and Nitrosomyces. Both types of bacteria are obligate aerobic bacte...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34B01J13/04
CPCB01J13/043C02F3/34C02F3/348
Inventor 王超陈亮胡晓宇
Owner 中科众沃生态科技(武汉)有限公司