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Surface-activated fluorescent latex microspheres and their preparation and application

A technology of fluorescent latex microspheres and surface activation, applied in fluorescence/phosphorescence, material analysis through optical means, instruments, etc., can solve problems such as agglomeration, and achieve the effects of easy operation, stable detection results, and accurate detection results

Active Publication Date: 2018-01-16
BEIJING ELCOTEQ BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve the above-mentioned technical problems, the present invention provides a surface-activated fluorescent latex microsphere, its preparation method and a dry fluorescent immunochromatographic test strip prepared by using the surface-activated fluorescent latex microsphere to solve the problem of fluorescent latex microspheres. When the balls are sprayed and dried on the glass cellulose membrane, the phenomenon of agglomeration occurs in order to prepare the marker pad, so as to obtain a dry fluorescent immunochromatography test strip, no diluent is required to configure the detection kit

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  • Surface-activated fluorescent latex microspheres and their preparation and application
  • Surface-activated fluorescent latex microspheres and their preparation and application
  • Surface-activated fluorescent latex microspheres and their preparation and application

Examples

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preparation example Construction

[0035] The invention provides a method for preparing surface-activated fluorescent latex microspheres, comprising the steps of:

[0036] 1) Add the carboxyl-containing surfactant to the buffer solution with a pH of 8~10, then add dimethylformamide, N,N'-dicyclohexylcarbodiimide (DCC, fat-soluble carbodiimide) and N-hydroxysuccinimide (NHS), stir to mix;

[0037] 2) Take the dispersion of fluorescent latex microspheres on the amino surface, adjust the pH to 8~10 with a buffer solution, add it to the product obtained in step 1), stir and react, after the reaction is completed, centrifuge to remove the supernatant to obtain the surface Activated fluorescent latex microspheres.

[0038] The invention adopts one or several carboxyl-containing surfactants to modify the outer surface of latex microspheres through covalent coupling to make them hydrophobic. The treated latex microspheres are re-labeled with the antibody to be tested using a suitable technique. Fluorescent latex mic...

Embodiment 1

[0042] Fluorescent Latex Microspheres Treated with Polyethylene Glycol Monolaurate

[0043] 1) Dissolve 10mg of polyethylene glycol monolaurate in 0.5M pH9.6 carbonate buffer (H value to 9.6), then add in 0.1ml of DMF, then add N, N'-dicyclohexylcarbodiimide (DCC) 1mg and 0.6mg N-hydroxysuccinimide (NHS) were stirred at room temperature for 3 hours.

[0044]2) Adjust the pH value of 1ml of fluorescent latex microsphere solution containing 1% solids on the amino surface to 9.6 with 0.5M carbonic acid buffer solution of pH 9.6, then add it to step 1), and continue to stir and react for 3 hours. Acquired activated latex microspheres.

[0045] 3) Use a high-speed centrifuge to centrifuge the fluorescent latex microsphere reaction solution at high speed, remove the supernatant of the reaction solution, and redissolve the fluorescent latex microspheres with 1ml microsphere buffer to form a marker working solution.

[0046] Microsphere phosphate buffer preparation: 0.01M pH7.4 phos...

Embodiment 2

[0048] Treatment of fluorescent latex microspheres with sodium stearate

[0049] The operation method of this example is similar to that of Example 1, except that in step 1), sodium stearate is used to replace polyethylene glycol monolaurate.

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Abstract

The invention discloses surface-activated fluorescent latex microspheres as well as a preparation method and application thereof. The preparation method of the surface-activated fluorescent latex microspheres comprises the following steps: 1) taking a methyloic surfactant, adding the methyloic surfactant into a buffer solution with a pH of 8-10, and then adding dimethyl formamide, N,N'-dicyclohexylcarbodiimide and N-hydroxy succinimide; and 2) taking dispersion liquid of latex microspheres with amino groups on the surfaces, regulating the pH of the buffer solution to 8-10, then adding the buffer solution into a mixture obtained in the step 1), stirring for reaction, and performing centrifugation to remove a supernatant after reaction, thereby obtaining the surface-activated fluorescent latex microspheres. During drying, the surface-activated fluorescent latex microspheres can keep relative distances among particles and are low in conglobation possibility; during a chromatography process, after a sample is added, re-dissolution is immediately realized, and chromatography is smoothly realized, so that a dry fluorescent immunity chromatography test paper strip (agent card) can be conveniently prepared, can be conveniently used by a user, and is stable, accurate and reliable in detection result.

Description

technical field [0001] The invention belongs to the technical field of fluorescence immunochromatography, and in particular relates to a surface-activated fluorescent latex microsphere and its preparation and application. Background technique [0002] Fluorescent immunochromatographic test strips are widely used in the field of quick detection because of their fast and convenient characteristics. The analyte antibody is labeled with fluorescent latex microspheres, and the latex microspheres have surface electron repulsion and van der Waals forces in the liquid phase to keep the latex particles in the liquid phase evenly distributed. However, after spraying on the glass cellulose film and drying, as the solution is evaporated to dryness, the surface tension pushes the latex particles infinitely closer together, resulting in the disappearance of the intermolecular repulsion, and the increase of the van der Waals force, which finally makes multiple latex molecules agglomerate i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J7/12C08L25/06G01N33/558G01N33/543G01N33/533G01N21/64
CPCC08J7/12C08J2325/06G01N21/6486G01N33/533G01N33/54313G01N33/558
Inventor 林斯
Owner BEIJING ELCOTEQ BIO TECH