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Hair shampoo product containing fermentation supernatant of lactobacillus

A hair shampoo product and fermentation supernatant technology, which is applied in cosmetics, hair care, cosmetic preparations, etc., can solve the problems of poor safety of chemical raw materials, lack of elasticity, dry and burnt hair color, etc., and achieve important application value, excellent anti-dandruff, Moisturizing and cleansing effect

Inactive Publication Date: 2017-02-15
北京蓝色美城科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The anti-dandruff and anti-itch function of hair shampoo products is due to the addition of substances with bactericidal effect in hair shampoo products, but in most cases, the added substances with bactericidal effect are chemical raw materials, and long-term use will cause hair splitting, Dry color and lack of elasticity, and the safety of chemical raw materials is poor

Method used

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  • Hair shampoo product containing fermentation supernatant of lactobacillus
  • Hair shampoo product containing fermentation supernatant of lactobacillus
  • Hair shampoo product containing fermentation supernatant of lactobacillus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1, the isolation and identification of lactic acid bacteria

[0046] 1. Isolation of bacteria

[0047] 1. Collection of samples

[0048] The vaginal secretions of women (all volunteers who gave informed consent to the test content) were collected as samples, a total of 10 samples.

[0049] 2. Isolation of bacteria

[0050] (1) Take a sample and dilute it to 10 times the volume with sterile water to obtain a sample dilution. Take 1mL sample diluent, stir and inoculate in solid medium at 30-40°C, and incubate at 37°C for 24-72h.

[0051] (2) After completing step (1), select a single colony, spot-inoculate it on a double-layer solid plate containing 1.6% (mass percentage) bromocresol violet, and culture it at 37°C for 24-72h.

[0052] (3) After completing step (2), select a single colony producing yellow pigment, streak and inoculate it on a double-layer solid plate containing 1.6% (mass percentage) bromocresol violet, and culture at 37°C.

[0053] (4) Aft...

Embodiment 2

[0060] Embodiment 2, the preparation of shampoo product

[0061] 1. Preparation of fermentation supernatant

[0062] 1. Take bacterial strain N1 and inoculate it into MRS medium (the initial bacterial concentration of lactic acid bacteria is 6×10 5 fu / mL), shake at 30°C (shaking speed: 220rpm, radius of rotation: 50mm) and culture for 24h, collect the whole culture system and name it system 1.

[0063] 2. After completing step 1, take system 1, inoculate it into MRS medium at a volume ratio of 1:9, and culture at 30°C for 24 hours with shaking (shaking speed: 220rpm, rotation radius: 50mm), collect the entire culture system, and name it system 2 .

[0064] 3. After completing step 2, take system 2, centrifuge at 4000rpm for 10min, and collect the supernatant. The supernatant was sterilized by filtration (filter pore size: 0.45 mm), and the fermentation supernatant of strain N1 was obtained.

[0065] According to the above method, replace strain N1 with strain N2, strain N3...

Embodiment 3

[0071] The shampoo effect of the shampoo product prepared by embodiment 3, embodiment 2

[0072] One, the antibacterial test of the shampoo product prepared in embodiment 2

[0073] According to the method of the antibacterial ring test of "Disinfection Technical Specification" 2002 edition 2.1.8.2, measure the bacteriostatic effect of the shampoo product to be tested to be tested bacterium. The shampoo products to be tested are shampoo product A1, hair shampoo product A2, hair shampoo product A3, hair shampoo product B1, hair shampoo product B2, hair shampoo product B3, hair shampoo product C1, hair shampoo product C2, and hair shampoo product C3 , hair shampoo D1, hair shampoo D2, hair shampoo D3, hair shampoo E1, hair shampoo E2, hair shampoo E3, hair shampoo F1, hair shampoo F2, hair shampoo F3, hair shampoo G1 , hair shampoo product G2, hair shampoo product G3 or hair shampoo product K. The tested bacteria were Staphylococcus aureus or Candida albicans. The antibacteri...

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Abstract

The invention discloses a hair shampoo product containing fermentation supernatant of lactobacillus. The hair shampoo product containing the fermentation supernatant of the lactobacillus provided by the invention consists of 10-30 parts by mass of the fermentation supernatant of the lactobacillus, 10.00-20.00 parts by mass of sodium laureth sulfate, 3.00-8.00 parts by mass of cocamidopropyl betaine, 0.10-0.20 part by mass of allantoin, 0.20-0.30 part by mass of quaternary ammonium salt-10, 0.20-0.50 part by mass of essence, 0.50-1.00 part by mass of a grease agent, 0.50-1.50 parts by mass of a thickening agent and 38.00-76.00 parts by mass of water. The lactobacillus is lactobacillus plantarum Rs0228, which is preserved in China General Microbiological Culture Collection Center with preservation number of CGMCC No.12881. Experiments prove that the hair shampoo product provided by the invention is excellent in functions of eliminating dandruff, relieving itching, moistening and cleaning, and the hair shampoo product has an important application value.

Description

technical field [0001] The invention belongs to the technical field of daily cosmetics, and in particular relates to a shampoo product containing fermented supernatant of lactic acid bacteria. Background technique [0002] Shampoo products are essential daily necessities in life. With the continuous improvement of living standards, people have higher and higher requirements for hair shampoo products, not only requiring shampoo products to have a good cleaning effect, but also to have the functions of removing dandruff and relieving itching. The anti-dandruff and anti-itch function of hair shampoo products is due to the addition of substances with bactericidal effect in hair shampoo products, but in most cases, the added substances with bactericidal effect are chemical raw materials, and long-term use will cause hair splitting, The hair color is dry and lacks elasticity, and the safety of chemical raw materials is poor. [0003] Lactic acid bacteria (LAB) is a general term ...

Claims

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Application Information

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IPC IPC(8): A61K8/99A61K8/86A61K8/49A61K8/44A61Q5/02A61Q5/00A61Q5/12A61P17/04
CPCA61K8/99A61K8/442A61K8/4946A61K8/86A61Q5/006A61Q5/02A61Q5/12
Inventor 孙筱霞那立亚
Owner 北京蓝色美城科技有限公司
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