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Stress active tissue engineering scaffold material and preparation method thereof

A tissue engineering scaffold and active technology, which is applied in the field of stress-activated tissue engineering scaffold materials and its preparation, to achieve the effects of accelerating the recovery process, ensuring biocompatibility, and improving biocompatibility

Inactive Publication Date: 2017-02-15
WUHAN TEXTILE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the above-mentioned problems, the purpose of the present invention is to solve the defects of existing tissue engineering scaffold materials, and provide a kind of tissue engineering scaffold material with stress activation and its preparation method

Method used

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  • Stress active tissue engineering scaffold material and preparation method thereof

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preparation example Construction

[0023] A method for preparing a stress-activated tissue engineering scaffold material, comprising preparation of polyvinylidene fluoride nanofiber membrane, preparation of silk fibroin solution or silk fibroin powder dispersion and polyvinyl alcohol solution, characterized in that the The preparation method proceeds as follows:

[0024] a. Take a polyvinylidene fluoride nanofiber membrane with a diameter of 50nm-1000nm, cut it, crush it, and ultrasonically disperse it in water to obtain a diameter of 50nm-1000nm and a length of 5000nm-100000nm with a mass concentration of 5wt%-20wt% polyvinylidene fluoride Fiber dispersion.

[0025] b. Mix the configured polyvinylidene fluoride nanofiber dispersion, silk fibroin solution or silk fibroin powder dispersion with polyvinyl alcohol solution to obtain a uniform and stable mixed suspension, wherein polyvinylidene fluoride and polyethylene The mass ratio of alcohol is 1 / 99~5 / 95, the mass ratio of silk fibroin and polyvinyl alcohol is...

Embodiment 1

[0033] a. Take the polyvinylidene fluoride nanofiber membrane with a diameter of 50nm, cut and pulverize it and ultrasonically disperse it in water to obtain a dispersion liquid with a diameter of 50nm and a length of 5000nm-100000nm with a mass concentration of 5wt% polyvinylidene fluoride nanofiber dispersion;

[0034] B. the polyvinylidene fluoride nanofiber dispersion liquid that mass concentration is 5wt%, the silk fibroin aqueous solution that mass concentration is 5wt% and the polyvinyl alcohol aqueous solution that mass concentration is 5wt% are mixed to obtain uniform and stable mixing suspension, wherein The mass ratio of polyvinylidene fluoride to polyvinyl alcohol is 1 / 99, the mass ratio of silk fibroin to polyvinyl alcohol is 5 / 95, and the total solute mass concentration of polyvinylidene fluoride, silk fibroin and polyvinyl alcohol is 5wt %.

[0035] c. Put the uniform and stable mixed suspension prepared in step b into a casting mold, freeze and solidify, demoul...

Embodiment 2

[0039] a. Take a polyvinylidene fluoride nanofiber membrane with a diameter of 500nm, cut and pulverize it and ultrasonically disperse it in water to obtain a polyvinylidene fluoride nanofiber dispersion with a mass concentration of 50000nm in diameter and a mass concentration of 10wt%.

[0040] B. the polyvinylidene fluoride nanofiber dispersion liquid that mass concentration is 10wt%, the silk fibroin aqueous solution that mass concentration is 10wt% and the polyvinyl alcohol aqueous solution that mass concentration is 10wt% are mixed to obtain uniform and stable mixing suspension, wherein The mass ratio of polyvinylidene fluoride to polyvinyl alcohol is 3 / 97, the mass ratio of silk fibroin to polyvinyl alcohol is 20 / 80, and the total solute mass concentration of polyvinylidene fluoride, silk fibroin and polyvinyl alcohol is 10wt %.

[0041] c. Put the uniform and stable mixed suspension prepared in step b into a casting mold, freeze and solidify, demould, and freeze-dry to ...

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Abstract

The invention relates to a stress active tissue engineering scaffold material prepared from polyvinylidene fluoride nanofiber, silk fibroin, polyvinyl alcohol, and a preparation method thereof, and belongs to the field of a biomedical material. In the stress active tissue engineering scaffold material, the mass ratio of the silk fibroin to the polyvinyl alcohol is 1 / 9 to 1 / 1; the mass ratio of the polyvinylidene fluoride nanofiber to the polyvinyl alcohol is 1 / 99 to 5 / 95. The preparation method is characterized in that polyvinylidene fluoride nanofiber suspension, silk fibroin solution or silk fibroin powder dispersion liquid and polyvinyl alcohol solution are mixed to obtain uniform and stable mixed suspension; casting mold forming, cryocoagulation, demoulding and freeze drying are performed to obtain cavernous bodies; then, gaseous esterification treatment is performed to prepare the stress active tissue engineering scaffold material. The stress active tissue engineering scaffold material has uniform and stable micro-structures and good biocompatibility; the substrate conditions of the biological tissue growth are provided; the piezoelectric stress response characteristics of the material suffering from external force are realized.

Description

technical field [0001] The invention relates to a stress-activated tissue engineering scaffold material and a preparation method thereof, belonging to the field of biomedical materials. Background technique [0002] Tissue engineering is an emerging interdisciplinary field developed in the past 30 years. The most basic idea is to isolate and culture cells in vitro and inoculate them on a scaffold with a certain spatial structure. The extracellular matrix forms tissues and organs with a certain structure and function. The scaffolds with a certain spatial structure are collectively called tissue engineering scaffolds, such as skin, bone, cartilage, tendons, ligaments, etc. As a tissue engineering scaffold, four conditions must be met: biocompatible materials suitable for cell adhesion and growth, pore structure for cell growth, degradation characteristics compatible with new tissue, and structural and mechanical properties that satisfy tissue engineering scaffolds. Scaffold m...

Claims

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Application Information

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IPC IPC(8): A61L27/26A61L27/50A61L27/56
CPCA61L27/26A61L27/50A61L27/56C08L27/16C08L29/04C08L89/00
Inventor 柏自奎李佳荣王洪吴星火周应山顾绍金陶咏真杨红军许杰刘洪涛徐卫林
Owner WUHAN TEXTILE UNIV