A kind of purification method of 7-dehydrocholesterol leftovers
A technology of dehydrocholesterol and purification method, which is applied in the field of purification of 7-dehydrocholesterol leftovers, can solve the problems of large solvent and energy consumption, environmental pollution, use, etc., and achieves high industrial feasibility, simple reaction process, and realization The effect of economic benefits
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Embodiment 1
[0023] Add 500ml of cyclohexane to 100.5g of 7-dehydrocholesterol scraps containing 10.2g of 7-dehydrocholesterol, heat up to 50°C to dissolve, and make a raw material solution with a concentration of 10g / ml; mix the raw material solution with an equal volume of sulfolane Mix evenly, oscillate in a constant temperature oscillator at 200r / min for 3h, and then stand at constant temperature for 2h. The extract of the upper layer was concentrated at a temperature of 40°C and a pressure of 0.095Mpa to 1 / 5 of the total amount of the original extract; the concentrated product was frozen and crystallized at a temperature of -20°C for 10 hours and then filtered to obtain purified 7-de Hydrocholesterol.
Embodiment 2
[0025] Add 450ml of cyclohexane to 95g of 7-dehydrocholesterol scrap containing 8.7g of 7-dehydrocholesterol, heat up to 40°C for dissolution, and make a raw material solution with a concentration of 15g / ml; mix the raw material solution with an equal volume of N- Mix the methylpyrrolidone evenly, shake it in a constant temperature oscillator at 250r / min for 3h, and then let it stand at constant temperature for 2h. The extract of the upper layer was concentrated at a temperature of 30°C and a pressure of 0.09Mpa to 1 / 5 of the total amount of the original extract; the concentrated product was frozen and crystallized at a temperature of -20°C for 10 hours, and then filtered to obtain purified 7-de Hydrocholesterol.
Embodiment 3
[0027] Add 310ml of cyclohexane to 50g of 7-dehydrocholesterol scrap containing 3.6g of 7-dehydrocholesterol, heat up to 45°C to dissolve, and make a raw material solution with a concentration of 20g / ml; mix the raw material solution with an equal volume of N, N-dimethylformamide was mixed evenly, shaken in a constant temperature oscillator at 250r / min for 3h, and then stood at constant temperature for 2h. The extract of the upper layer was concentrated at a temperature of 30°C and a pressure of 0.09Mpa to 1 / 6 of the total amount of the original extract; the concentrated product was frozen and crystallized at a temperature of -20°C for 10 hours, and then filtered to obtain purified 7-de Hydrocholesterol.
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