Method for extracting microcystis toxin MC-LR in algae cells

Abstract

The invention provides a method for extracting microcystis toxin MC-LR in algae cells. According to the method, firstly, the algae cells are obtained through freeze centrifugation; then, ultra-pure water is used for cleaning; next, extraction liquid is added; after the oscillation, the materials are transferred into a graduated test tube, and a centrifugation tube is cleaned three times by the extraction liquid; then, 25min of boiling bath is performed for MC-LR extraction; if the extraction liquid is not completely volatilized when the boiling water bath time reaches 25min, the time can be properly prolonged, but the total extraction time cannot exceed 30min; after the extraction is completed, the materials are cooled to room temperature; 50 percent of methanol is added for oscillation dissolution; after the filtering by a 0.22 mu m organic phase filter head, the determination is performed by a high performance liquid chromatography device. The method has the advantages that fewer simple devices can be used for achieving the high efficiency of extracting the microcystis toxin MC-LR in the cells by fewer experiment steps; the method belongs to a simple and easy-to-implement method for extracting the intracellular microcystis toxin MC-LR.

Description

technical field [0001] The invention relates to a method for extracting microcystin MC-LR in algal cells. Background technique [0002] Eutrophication of water body has become a common problem faced by current water body management, and the overgrowth of algae is the main reason for eutrophication. In freshwater lakes, the most direct manifestation of water eutrophication is algal bloom, and Microcystis aeruginosa is a common dominant algal species in the process of algal bloom outbreak. Compared with the algal blooms caused by other algae, the outbreak of Microcystis aeruginosa has its particularity. More and more research results show that the bloom of Microcystis aeruginosa is usually accompanied by the bloom of algal toxins. Microcystis aeruginosa can synthesize three algal toxins, MC-RR, MC-YR, and MC-LR, among which MC-LR has the largest synthesis amount and the strongest toxic effect. [0003] The results of research on the synthesis and release of algae toxins sho...

AI Technical Summary

Problems solved by technology

Although the freeze-thaw method does not require special equipment and has low requirements for equipment, it takes a long time to freeze-thaw the broken cells, the operation is cumbersome, repeated freezing and thawing is required, and the cell breaking efficiency is actually not very high, reaching 90% is ideal state; although the ultrasonic crushing operation time is relatively short, there is the same problem of crushing efficiency as the freeze-thaw method. In addition, the ultrasonic crushing method will cause many other substances in the cell, such as chlorophyll and various macromolecular cellular substances to enter into the cell. In the solution, it will interfere with the determination of algae toxins; compared with the cell crushing method, although the boiling water bath extraction method overcomes the disadvantages of low extraction efficiency, cumbersome operation, and many interfering substances, this method requires special equipment, and nitrogen gas is still required for subsequent processing. escape

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