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Achromobacter xylosoxidans for preventing and treating plant root-knot nematode and application of achromobacter xylosoxidans

A colorless bacillus and root-knot nematode technology, applied in the direction of chemicals, applications, and pest control for biological control, can solve the problems that the research on plant nematode diseases has not been reported, and achieve low cost and good promotion. The effect of production and pollution reduction

Active Publication Date: 2017-02-15
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] So far, there are many reports on the use of bacteria to control nematode diseases, but the research on the use of Achromobacter xylosoxidans to control plant nematode diseases has not been reported

Method used

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  • Achromobacter xylosoxidans for preventing and treating plant root-knot nematode and application of achromobacter xylosoxidans
  • Achromobacter xylosoxidans for preventing and treating plant root-knot nematode and application of achromobacter xylosoxidans
  • Achromobacter xylosoxidans for preventing and treating plant root-knot nematode and application of achromobacter xylosoxidans

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Isolation and Identification of AH-5 Strain

[0034] 1.1 Isolation of AH-5 strain

[0035] The applicant isolated and screened a strain with strong control effect on root-knot nematode from soil samples in Anhui Province, China, and named it AH-5. The isolation and purification of the AH-5 bacterial strain of the present invention adopt methods such as dilution coating plate method, plate streaking method, biological assay method and the like.

[0036] 1.2 Identification of strains

[0037] 1.2.1 Morphological identification of strains

[0038] By observing the color, shape, edge, transparency, glossiness, color of the culture medium, etc. of the colony, and observing the individual morphological characteristics by Gram staining.

[0039] Streak culture of AH-5 strain on beef extract peptone medium plate, from figure 1 It can be seen in: AH-5 colonies are light yellow, translucent, raised, dull, with slightly neat edges, and no pigment. Microscopic examination foun...

Embodiment 2

[0053] Effect of Fermentation Supernatant of AH-5 Strain on Hatching of Meloidogyne Eggs

[0054] Add 700 μL of AH-5 supernatant to each well of the 24-well plate, and at the same time add 100 root-knot nematode eggs to each well and mix them evenly with the bacterial supernatant, repeat three times, set sterile water as a control; The 24-well plates of Knot nematode eggs and AH-5 supernatant were placed in a 25°C incubator, observed and counted under a stereomicroscope after 7 days, and the hatching rate and relative inhibition rate of root-knot nematode eggs were calculated.

[0055] Effect of table 2AH-5 fermentation supernatant on hatching of root-knot nematode eggs (7d, 25°C)

[0056]

[0057] Note: Different letters in the table mean significant difference (SPSS 17.0t test, P≤0.05)

[0058] The results of incubation of root-knot nematode eggs with AH-5 strain fermentation broth showed that: with ddH 2 O is that the hatching rate of CK of control group reaches 86.5% ...

Embodiment 3

[0060] Effects of Fermentation Supernatant of AH-5 Strain on Second Instar Larvae of Meloidogyne Nematode

[0061] Add 700 μL of AH-5 supernatant to each well of the 24-well plate, add 100 root-knot nematode second-instar larvae to each well and mix with the supernatant evenly, repeat three times, and set sterile water as a reference at the same time. The 24-well plates of root-knot nematode eggs and AH-5 supernatant were placed in a 25°C incubator, observed under a stereomicroscope after 12 hours and 24 hours respectively, and the results were recorded.

[0062] The influence of table 3AH-5 fermentation supernatant on root-knot nematode second instar larvae (25 ℃)

[0063]

[0064] Note: Different letters in the table mean significant difference (SPSS 17.0t test, P≤0.05)

[0065] The experimental results of the effect of AH-5 strain fermentation supernatant on root-knot nematode second-instar larvae showed that the second-instar larvae did not die at 12h and 24h after ddH...

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Abstract

The invention discloses achromobacter xylosoxidans for preventing and treating plant root-knot nematode and application of the achromobacter xylosoxidans. The achromobacter xylosoxidans is Achromobacter xylosoxidans AH-5 CCTCC NO:M 2016458. In an indoor nematicidal activity determination test, the fatality rate of AH-5 strain fermentation supernate for second-stage nematode juveniles in 24 hours can reach 92.4%; through an indoor pot test result, it is shown that the number of root-knots of pea roots of an AH-5 fermentation bacteria liquid processing set is remarkably reduced, and the control effect can reach 72.29% 40 days later. In a field experiment of pea root-knot nematode disease, the number of root-knots of pea roots of an AH-5 processing set is remarkably reduced, and the control effect of the root-knot nematode disease in 120 days is 43.59%; the fermentation bacteria liquid of the achromobacter xylosoxidans can have effects of promoting pea plant growth and root lengthening and is a strain of nematode disease bio-control bacteria with great development potential.

Description

technical field [0001] The invention belongs to the technical field of biological control of plant diseases, and in particular relates to an achromobacter for preventing and controlling plant nematode diseases and an application thereof. Background technique [0002] Root-knot nematode is considered to be one of the most important pathogens in the world because of its worldwide geographical distribution and wide host range, causing serious harm to many important economic crops. The most common species is estimated to infect over 5,500 plant species. The hosts of root-knot nematodes are found in food, oil plants, vegetables, fruit trees and other crops, such as tomato, lettuce, cucumber, peanut, tobacco, citrus and other plants can be infected by root-knot nematode. After the disease of the plant root system, multiple roots join together to form tumors of different sizes. In the late stage of the disease, the roots become rough and easy to rot. Root deformities lead to dama...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P5/00A01P21/00C12R1/025
CPCA01N63/00A01N63/10C12N1/205C12R2001/025
Inventor 肖炎农袁和奇黄豆豆王高峰肖雪琼
Owner HUAZHONG AGRI UNIV
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