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A pretreatment method for the analysis of aroma components in reconstituted tobacco leaves

A technology for reconstituted tobacco leaves and aroma components, applied in the analysis of materials, material separation, instruments, etc., can solve the problems of long time, large solvent, etc., and achieve the effect of good repeatability, easy operation, and short time

Active Publication Date: 2019-04-26
HUBEI CHINA TOBACCO IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method needs to consume a large amount of solvent (generally 40mL dichloromethane) and a long time (generally 2h)

Method used

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  • A pretreatment method for the analysis of aroma components in reconstituted tobacco leaves
  • A pretreatment method for the analysis of aroma components in reconstituted tobacco leaves
  • A pretreatment method for the analysis of aroma components in reconstituted tobacco leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Weigh 5g of the shredded reconstituted tobacco sample into a conical flask, add 80mL of ultrapure water, immerse the sample, after ultrasonication for 10min, let it stand for 12h, shake it well, take the filtrate, and obtain the water-extracted reconstituted tobacco sample;

[0024] 2. Weigh 5g of shredded reconstituted tobacco leaf sample into a conical flask, add 80mL of absolute ethanol, immerse the sample, after ultrasonication for 10min, let it stand for 12h, shake and shake well, then take the filtrate to obtain the alcohol-extracted reconstituted tobacco leaf sample;

[0025] 3. Measure 10mL water-extracted reconstituted tobacco leaf sample into a 50mL centrifuge tube with a pointed bottom stopper, use a syringe to quickly inject a dichloromethane / acetonitrile mixture (1.0mL dichloromethane and 1.5mL acetonitrile mixture), oscillate gently for 1min, Then centrifuge at 4000r / min for 5min, and finally use a micro-sampler to absorb the sediment phase and pass it t...

Embodiment 2

[0038] 1. Weigh 5g of shredded reconstituted tobacco leaf sample into a conical flask, add 60mL of ultrapure water, immerse the sample, after ultrasonication for 10min, let it stand for 12h, shake it well, take the filtrate, and obtain the water-extracted reconstituted tobacco leaf sample;

[0039] 2. Weigh 5g of shredded reconstituted tobacco leaf sample into a conical flask, add 60mL of absolute ethanol, immerse the sample, after ultrasonication for 10min, let it stand for 12h, oscillate and shake well, take the filtrate, and obtain the ethanol-extracted reconstituted tobacco leaf sample;

[0040] 3. Measure 10mL water-extracted reconstituted tobacco leaf sample into a 50mL centrifuge tube with a pointed bottom stopper, use a syringe to quickly inject a dichloromethane / acetonitrile mixture (1.0mL dichloromethane and 1.5mL acetonitrile mixture), oscillate gently for 1min, Then centrifuge at 4000r / min for 5min, and finally use a micro-sampler to absorb the sediment phase and pa...

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Abstract

The invention provides a pretreatment method for analysis of aroma components of reconstituted tobaccos. The pretreatment method includes steps: (1) subjecting the reconstituted tobaccos to water extraction; (2) subjecting the reconstituted tobaccos to alcohol extraction; (3) measuring 10mL of a sample of the reconstituted tobaccos subjected to water extraction into a 50mL sharp-bottomed centrifuge tube with a plug, using an injector for quickly injecting methylene dichloride / acetonitrile mixed liquid, gently and ultrasonically vibrating for 1min, centrifuging for 5min at a speed of 4000r / min, adopting a microsyringe for sucking a sedimentary phase, and filtering through a 0.22micron filter film to perform GC-MS (gas chromatography-mass spectrometer) analysis; (4) treating a sample of the reconstituted tobaccos subjected to alcohol extraction according to the step (3). The method is easy in operation, short in time and low in organic solvent consumption, various components different in polarity can be separated, experimental efficiency can be improved evidently, and high repeatability is realized.

Description

technical field [0001] The invention relates to the technical field of reconstituted tobacco leaves, in particular to a pretreatment method for analyzing the aroma components of reconstituted tobacco leaves. Background technique [0002] Reconstituted tobacco, also known as tobacco flakes or reconstituted tobacco, is a product of recycling tobacco waste resources. It mainly uses tobacco by-products and tobacco waste such as tobacco stems, tobacco powder, chips, and shreds. Kraft paper-like reconstituted tobacco products processed by hand. [0003] Previous analysis studies have shown that the aroma components in reconstituted tobacco leaves cannot be analyzed by direct extraction pretreatment method, and simultaneous distillation extraction method is required. At the same time, the distillation extraction method is a very effective means to extract the volatile and semi-volatile components in tobacco leaves, and has a good recovery rate and reproducibility for the extractio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 刘吟谭烨邵兴伟曹林海
Owner HUBEI CHINA TOBACCO IND