Solid-phase synthesis method for preparing exenatide
A technology for exenatide and solid-phase synthesis, which is applied in the preparation methods of peptides, chemical instruments and methods, peptides, etc., can solve the problems of complex synthesis steps, difficult separation in the later stage, and difficult coupling, etc., and achieves reduction of residual peptides. The formation of impurities, the elimination of β-sheet secondary space structure, and the effect of improving yield
Inactive Publication Date: 2017-02-22
滨海吉尔多肽有限公司 +1
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The invention discloses a method for preparing exenatide, aiming at mainly solving the technical problems in the prior art that synthesis steps are complicated, the coupling difficulty is great, post-period separation is difficult, and the like. According to the technical scheme, the method comprises the following steps: 1) mixing Fmoc-Rink Amide Linder and PEG (Polyethylene Glycol) Matrix resin, and reacting to obtain Fmoc-Rink Amide PEG Matrix resin; 2) gradually coupling amino acids with protective groups and proline pseudodipeptide segments by adopting a Fmoc solid-phase synthesis method, so as to obtain side-chain full-protection polypeptide resin; and 3) cutting the side-chain full-protection polypeptide resin, cracking polypeptides from the resin, removing side-chain protection groups so as to obtain an exenatide crude product, and carrying out high performance liquid chromatography separation and purification and freeze-drying on the crude peptide to obtain the exenatide. The process method for preparing the exenatide, provided by the invention, has the characteristics of simplicity and convenience for operation, high crude peptide purity and high comprehensive yield.
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