Batch production method of medicinal dioscorea zingiberensis

A technology of Dioscorea Shield leaf and Dioscorea Shield leaf, which is applied in the field of efficient mass production of Dioscorea Shield leaf, can solve the problems of low survival rate of test-tube seedling transplanting, small number of effective seedlings and high cost per plant, and achieves reduction of no The effect of bacteria operation steps, accelerated reproduction, and short seedling period
CN106472318AInactive Publication Date: 2017-03-08INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI +1
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI
Publication Date
2017-03-08
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The invention discloses an effective and low-energy-consumption rapid propagation method of dioscorea zingiberensis. The method comprises steps as follows: (1) stems of an excellent medicinal dioscorea zingiberensis plant are selected as explants, the explants are disinfected and then cultured in an agar solid culture medium containing MS, 6-BA (0.01-0.05 mg / L), paclobutrazol (3.0-10.0 mg / L) and chlormequat chloride (3.0-10.0 mg / L) and having the content of components excluding inositol unchanged and the content of inositol doubled, and a large quantity of small bud clusters are induced; (2) the cluster buds obtained in the step (1) are cut off and divided into clusters, each cluster comprises five buds, an agar solid culture medium containing MS, NAA (0.02-0.6 mg / L), IAA (0.02-0.6 mg / L), paclobutrazol (0.5-5.0 mg / L) and chlormequat chloride (0.5-5.0 mg / L) and having the content of components excluding inositol unchanged and the content of inositol doubled is inoculated with the buds for proliferation and rooting. The method is good in cultivation effect, high in proliferation coefficient, good in stability and low in production cost and is a rapid propagation method for obtaining a large quantity of test-tube plantlets within the shortest time.
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Description

technical field

[0001] The invention belongs to the technical field of tissue culture of medicinal Dioscorea scutellaria, and in particular relates to an efficient batch production method of medicinal Dioscorea scutellaria. Background technique

[0002] Dioscorea plants (Dioscorea) have important medicinal value, and their rhizogenin is an important starting material for the synthesis of contraceptives and steroid hormones, and plays an important role in the pharmaceutical industry. In the "List of Rare and Endangered Plants in China", it is listed as a national second-class endangered species and is protected. The large-scale tissue culture, rapid propagation and standardized planting of salvia hispanica L by plant cell engineering technology and methods play an important role in promoting the development of the pharmaceutical industry. In 2014, a batch production method of one-step seedling growth of medicinal Dioscorea scutellaria was provided (authorization number ZL201...

Claims

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