Batch production method of medicinal dioscorea zingiberensis

A technology of Dioscorea Shield leaf and Dioscorea Shield leaf, which is applied in the field of efficient mass production of Dioscorea Shield leaf, can solve the problems of low survival rate of test-tube seedling transplanting, small number of effective seedlings and high cost per plant, and achieves reduction of no The effect of bacteria operation steps, accelerated reproduction, and short seedling period

Inactive Publication Date: 2017-03-08
INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2014, a batch production method of one-step seedling growth of medicinal Dioscorea scutellaria was provided (authorization number ZL201410288573.9), which can simultaneously induce callus, buds, and adventitious roots, and simplify multiplication and rooting in one step. The seedlings need to go through the stage of callus culture, which increases the mutation rate
In 2015, a method for rooting of aseptic short shoots of medicinal Dioscorea ...

Method used

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  • Batch production method of medicinal dioscorea zingiberensis
  • Batch production method of medicinal dioscorea zingiberensis
  • Batch production method of medicinal dioscorea zingiberensis

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Embodiment 1

[0026] The following examples are intended to further illustrate the present invention, rather than limit the present invention. Embodiment 1, tissue culture rapid propagation Dioscorea shield leaf

[0027] 1. Culture medium preparation and sterilization

[0028] The first-generation subculture medium is used for the propagation of basic seedlings: MS improvement (the content of other MS elements remains unchanged, only the content of inositol is doubled) + 6-BA 0.05mg / L + paclobutrazol 6.0mg / L + chlormequat 3.0mg / L;

[0029] Elongation and rooting medium for mass production: MS improvement (MS other element content remains unchanged, only inositol content doubled) + NAA0.1mg / L + IAA0.2mg / L + paclobutrazol 1.0mg / L + chlormequat 2.0mg / L;

[0030] The above medium was sterilized at 121°C for 20 minutes.

[0031] 2. Efficient basic seedling propagation

[0032] Take the stem section of salvia hispanica L, first wash it with washing powder, wash it with sterile water for 5 to...

Embodiment 2

[0038] Embodiment 2, tissue culture rapid propagation Dioscorea shield leaf

[0039] 1. Culture medium preparation and sterilization

[0040] First-generation subculture medium: MS improvement (the content of other MS elements remains unchanged, only the content of inositol is doubled) + 6-BA0.02mg / L + paclobutrazol 4.0mg / L + chlormequat 6.0mg / L;

[0041] Elongation and rooting medium: MS improvement (the content of other MS elements remains unchanged, only the content of inositol is doubled) + NAA0.2mg / L + IAA0.3mg / L + paclobutrazol 1.5mg / L + chlormequat 1.0mg / L.

[0042] The above medium was sterilized at 121°C for 20 minutes.

[0043] Get the stem section of yam yam (salvia hispanica L), the basic operation method is consistent with embodiment 1; the effect is not much different from embodiment 1.

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Abstract

The invention discloses an effective and low-energy-consumption rapid propagation method of dioscorea zingiberensis. The method comprises steps as follows: (1) stems of an excellent medicinal dioscorea zingiberensis plant are selected as explants, the explants are disinfected and then cultured in an agar solid culture medium containing MS, 6-BA (0.01-0.05 mg/L), paclobutrazol (3.0-10.0 mg/L) and chlormequat chloride (3.0-10.0 mg/L) and having the content of components excluding inositol unchanged and the content of inositol doubled, and a large quantity of small bud clusters are induced; (2) the cluster buds obtained in the step (1) are cut off and divided into clusters, each cluster comprises five buds, an agar solid culture medium containing MS, NAA (0.02-0.6 mg/L), IAA (0.02-0.6 mg/L), paclobutrazol (0.5-5.0 mg/L) and chlormequat chloride (0.5-5.0 mg/L) and having the content of components excluding inositol unchanged and the content of inositol doubled is inoculated with the buds for proliferation and rooting. The method is good in cultivation effect, high in proliferation coefficient, good in stability and low in production cost and is a rapid propagation method for obtaining a large quantity of test-tube plantlets within the shortest time.

Description

technical field [0001] The invention belongs to the technical field of tissue culture of medicinal Dioscorea scutellaria, and in particular relates to an efficient batch production method of medicinal Dioscorea scutellaria. Background technique [0002] Dioscorea plants (Dioscorea) have important medicinal value, and their rhizogenin is an important starting material for the synthesis of contraceptives and steroid hormones, and plays an important role in the pharmaceutical industry. In the "List of Rare and Endangered Plants in China", it is listed as a national second-class endangered species and is protected. The large-scale tissue culture, rapid propagation and standardized planting of salvia hispanica L by plant cell engineering technology and methods play an important role in promoting the development of the pharmaceutical industry. In 2014, a batch production method of one-step seedling growth of medicinal Dioscorea scutellaria was provided (authorization number ZL201...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 黄丽芳夏新界杨平辉刘永源欧阳敏罗晓江
Owner INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI
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