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Plum blossom callus induction culture medium

A technology for inducing culture medium and callus, applied in the field of plant tissue culture, can solve the problems of browning, explant culture browning, mycelium infection, etc., achieve light green color, reduce browning, and promote rapid formation. Effect

Inactive Publication Date: 2017-03-08
GUANGXI NANYIZAI SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the invention of the present invention is to provide a method that can solve the problems of browning and mycelial infection in the prior art to solve the problems of browning and mycelial infection in the explant culture process, thereby improving Methods for quality and induction rate of plum blossom leaf callus

Method used

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  • Plum blossom callus induction culture medium
  • Plum blossom callus induction culture medium

Examples

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Comparison scheme
Effect test

Embodiment 1

[0022] The induction medium of plum blossom callus provided by the invention and preparation method thereof are as follows:

[0023] A kind of induction medium of plum blossom callus, by weight, comprises the following substances: 100 parts of MS culture medium, 20 parts of mother liquor I and 10 parts of purified water; In the described mother liquor I, include the substance of following mass-volume concentration: Calcium hydrogen sulfate 1mg / L, isopentenylaminopurine 2mg / L, 4-iodophenoxyacetic acid 2mg / L, water-soluble azone 1mg / L and propiconazole 1mg / L.

[0024] The preparation method of mother liquor I is as follows:

[0025] Weigh calcium bisulfite 1mg, isopentenylaminopurine 2mg, 4-iodophenoxyacetic acid 2mg, water-soluble azone 1mg and propiconazole 1mg; then, calcium bisulfite, isopentenylaminopurine, 4- Add iodophenoxyacetic acid, water-soluble azone, water-soluble azone and propiconazole respectively into purified water, and fully stir until calcium bisulfite, isop...

Embodiment 2

[0031] The induction medium of plum blossom callus provided by the invention and preparation method thereof are as follows:

[0032] A kind of induction medium of plum blossom callus is characterized in that, by weight ratio, comprises the following substances: 115 parts of MS medium, 25 parts of mother liquor I and 15 parts of purified water; The following mass-volume concentration is included in the mother liquor I Substances: calcium bisulfite 3mg / L, prenylaminopurine 4mg / L, 4-iodophenoxyacetic acid 4mg / L, water-soluble azone 2.5mg / L and propiconazole 2.5mg / L.

[0033] The preparation method of mother liquor I is as follows:

[0034] Weigh calcium bisulfite 3mg, amrenylaminopurine 4mg, 4-iodophenoxyacetic acid 4mg, water-soluble azone 2.5mg and propiconazole 2.5mg; then, calcium bisulfite, amrenylaminopurine, Add 4-iodophenoxyacetic acid, water-soluble azone, water-soluble azone and propiconazole respectively into purified water, and fully stir until calcium bisulfite, iso...

Embodiment 3

[0040] The induction medium of plum blossom callus provided by the invention and preparation method thereof are as follows:

[0041]A kind of induction culture medium of plum blossom callus, by weight, comprises the following substances: 130 parts of MS medium, 30 parts of mother liquor I and 20 parts of purified water; In the described mother liquor I, include the following mass-volume concentration of substances: Calcium hydrogen sulfate 5mg / L, isopentenylaminopurine 6mg / L, 4-iodophenoxyacetic acid 6mg / L, water-soluble azone 4mg / L and propiconazole 4mg / L.

[0042] The preparation method of mother liquor I is as follows:

[0043] Weigh calcium bisulfite 5mg, isopentenylaminopurine 6mg, 4-iodophenoxyacetic acid 6mg, water-soluble azone 4mg and propiconazole 4mg; then, calcium bisulfite, isopentenylaminopurine, 4- Add iodophenoxyacetic acid, water-soluble azone, water-soluble azone and propiconazole respectively into purified water, and fully stir until calcium bisulfite, isop...

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Abstract

The invention belongs to the field of plant tissue culture, and particularly relates to a plum blossom callus induction culture medium. The culture medium comprises the following substances in parts by weight: 100-130 parts of MS (Murashige-Skoog) culture medium, 20-30 parts of mother solution I and 10-20 parts of pure water. The mother solution I comprises the following substances in mass-volume concentration: 1-5 mg / L calcium bisulfite, 2-6 mg / L isopentene aminopurine, 2-6 mg / L 4-iodophenoxyacetic acid, 1-4 mg / L water-soluble azone and 1-4 mg / L propiconazole. The culture medium is capable of quickly inducing the formation of plum blossom calluses, preventing the browning phenomenon in the plum blossom tissue culture process and enhancing the callus inductivity and callus quality, has the advantage of short callus induction time, and thus, has great popularization meanings and wide application value.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and in particular relates to an induction medium of plum blossom callus. Background technique [0002] Plum blossom is a traditional famous flower in my country. It has a history of more than 7,000 years of application and a history of cultivation of more than 3,000 years in my country. garden landscape. It only takes about two or three months for plum blossoms to mature from pollination, and the fruit development time is short. When the fruit reaches maturity, the embryo is not yet mature. The seeds have been stored in sand, and the germination rate is also very low under normal conditions. Callus culture is not only a new method for rapid plant propagation, but also an ideal way for plant improvement, germplasm preservation and production of useful compounds. Meihua is a woody plant, and the induction of its callus is relatively difficult. During the tissue culture process, it is often Pro...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 覃永红
Owner GUANGXI NANYIZAI SCI & TECH CO LTD
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