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Application of protein of high-pathogenicity staphylococcus hyicus to subunit vaccine

A technology of Staphylococcus suis and subunit vaccines, applied in the field of biomedicine, can solve the problems of no relevant reports on the protein level of Staphylococcus suis, and achieve the effect of preventing Staphylococcus suis infection and enhancing resistance

Inactive Publication Date: 2017-03-08
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on Staphylococcus suis is mainly based on the gene level and the cell level, and there are few studies on the protein level, and there is no relevant report on the research on the protein level of Staphylococcus suis.

Method used

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  • Application of protein of high-pathogenicity staphylococcus hyicus to subunit vaccine
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  • Application of protein of high-pathogenicity staphylococcus hyicus to subunit vaccine

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Embodiment Construction

[0023] The application of a highly pathogenic Staphylococcus suis protein as a subunit vaccine for preventing porcine exudative epidermatitis caused by Staphylococcus suis infection. The amino acid sequence of the protein of Staphylococcus suis is shown in SEQ ID NO:1.

[0024] The method for preparing the highly pathogenic Staphylococcus suis protein as a subunit vaccine is as follows: adding Freund's adjuvant, excipient and freeze-drying stabilizer to the secreted protein of Staphylococcus suis, and stirring evenly.

[0025] The preparation method of the protein of described highly pathogenic Staphylococcus suis is:

[0026] 1) Bacterial culture: Streak and inoculate frozen Staphylococcus suis (S. hycius, named ZC-4) strains on LB plates, and shake culture at 37°C for about 12 hours. 2) Genomic DNA extraction: Genomic DNA was extracted from the above-mentioned cultured bacteria liquid according to the bacterial genome extraction reagent method;

[0027] 2) Genomic DNA extr...

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Abstract

The invention discloses application of protein of high-pathogenicity staphylococcus hyicus to a subunit vaccine. The secreted protein has a relatively good immune protection capability and can resist infection on mice by the staphylococcus hyicus. The amino acid sequence of the protein of the staphylococcus hyicus is shown as SEQ ID NO: 1. The protein acetate kinase of the staphylococcus hyicus (S.hyicus, named as ZC-4) has relatively good immunogenicity; prokaryotic expression recombinant protein and pig immune blood serum of the ZC-4 have a mutual effect, and specificity with a relatively high level can be induced. A toxin attacking protection efficacy experiment shows that the resistance on the staphylococcus hyicus of the mice and piglets can be improved through the protein, and the infection of the staphylococcus hyicus can be effectively prevented through taking the protein as the subunit vaccine.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of the protein of highly pathogenic Staphylococcus suis as a subunit vaccine. Background technique [0002] Porcine exudative epidermatitis is an acute fatal superficial pyodermatitis caused by Staphylococcus suis infection in suckling piglets or early weaned piglets. It is characterized by systemic oily exudative dermatitis and is highly contagious. Exudative dermatitis occurs in suckling piglets and weaned piglets in most countries in the world, with an incidence of 10% to 90% and a mortality rate of 5% to 90%. In recent years, the incidence of porcine exudative dermatitis in pig farms at home and abroad has gradually increased. Although there have been many studies on Staphylococcus suis at home and abroad, its virulence and pathogenic mechanism are still not very clear. [0003] In 2011, our laboratory isolated a strain of highly pathogenic Staphylococcus...

Claims

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Application Information

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IPC IPC(8): A61K39/085A61P31/04
CPCA61K39/085A61K2039/552
Inventor 宋长绪王磊张乐宜刘燕玲梁鹏帅郭亚星
Owner SOUTH CHINA AGRI UNIV
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